Expression of annexin A5 in serum and tumor tissue of patients with colon cancer and its clinical significance

Abstract

AIMTo investigate the expression of annexin A5 in serum and tumor tissue of patients with colon cancer and to analyze its clinical significance.METHODSNinety-three patients with colon cancer treated at our hospital between February 2013 and March 2016 were included in an observation group, and 40 healthy individuals were included in a control group. Enzyme-linked immunosorbent assay was performed to determine the serum level of annexin A5, while immunohistochemistry was performed to determine the expression of annexin A5 in cancer tissues.RESULTSThe serum level of annexin A5 was 0.184 ± 0.043 ng/mL in the observation group, which was significantly higher than that in the control group (P < 0.05). Annexin A5 expression was detected in 79.31% of the patients with lymph node metastasis, which was significantly higher than that in patients without lymph node metastasis (P < 0.05). Moreover, annexin A5 expression was detected in 86.96% of the patients with stage III to IV disease, which was significantly higher than that in patients with stage I to II disease (P < 0.05). The serum level of annexin A5 was 0.215 ± 0.044 ng/mL in patients whose tumors were positive for annexin A5 expression, which was significantly higher than that in patients whose tumors were negative for annexin A5 expression (P < 0.05). The serum level of annexin A5 was correlated with annexin A5 expression in colon cancer tissues (r = 0.312, P < 0.05). When a cutoff value of > 0.148 ng/mL for serum level of annexin A5 was used in the diagnosis of colon cancer, the sensitivity was 83.90%, and the specificity was 57.50%.CONCLUSIONFor patients with colon cancer, annexin A5 expression in cancer tissues is related to lymph node metastasis and tumor grade. Serum level of annexin A5 is related to annexin A5 expression in cancer tissues and is of diagnostic relevance.

Cross-Species Proteomics Identifies CAPG and SBP1 as Crucial Invasiveness Biomarkers in Rat and Human Malignant Mesothelioma

“…The potential of the smallest member of the annexin family as a predictive biomarker for tumor development, metastasis, and invasion has already been reviewed [ 41 ], with it also being involved in cell membrane repair [ 42 ]. Our results are consistent with reports of its overexpression in several other cancer types, including renal cell carcinoma [ 43 ], colon cancer [ 44 ], and hepatocarcinoma [ 45 , 46 ]. Other highly significant changes mainly involve two proteins, COX2 for increase and VAT1 for decrease.…”

S100A4 Is a Biomarker of Tumorigenesis, EMT, Invasion, and Colonization of Host Organs in Experimental Malignant Mesothelioma

“…Other than S100A4, our study also led us to identify several other potential markers of interest for MM, including annexin A5 [60], as its serum level in patients with colon cancer is related to lymph node metastasis and tumor grades [61], while investigations of its role and action in hepatocarcinoma malignancy has revealed that its knockdown suppressed the expression of key molecules in the integrin and MEK-ERK pathways (mitogen-activated protein kinases-extracellular signal-regulated kinases) [62]. Regarding lamin A/C, Kim et al demonstrated that it forms a perinuclear apical actin cap, resisting nuclear deformation in response to physiological mechanical stresses [63].…”

OmicsARules: a R package for integration of multi-omics datasets via association rules mining

“…These two rules suggested a combined mechanism between TAP1 expression and DNA methylation of ANXA5/KDM5B, which leads to the abnormal expression of PSMB9 in ESCA. ANXA5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and it plays a role in cellular signal transduction, inflammation, growth and differentiation [20]. An association between ANXA5 and several cancers has been assumed [20].…”

“…ANXA5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and it plays a role in cellular signal transduction, inflammation, growth and differentiation [20]. An association between ANXA5 and several cancers has been assumed [20]. KDM5B is the specific demethylase of histone H3 lysine 4 (H3K4), and its’ up-regulation can reduce H3K4 methylation level.…”