Expression of annexin II in human atherosclerotic abdominal aortic aneurysms

Proteomic analysis of the abdominal aortic aneurysm wall

“…For several proteins, the finding of discrepant expression in the aneurismal and healthy corresponding atherosclerotic aortic wall was in accordance with previous reports (the differential expression was detected various methods), and included alpha-1-antitrypsin [6], alcohol dehydrogenase [7], annexins [8, 9] and glyceraldehyde-3-phosphate dehydrogenase [9]. In other cases, an association of the specific protein with aneurysm formation is biologically plausible, especially in the case of extracellular matrix and cytoskeleton components.…”

Expression of annexin II in experimental abdominal aortic aneurysms

“…We recently demonstrated various levels of annexin II expression within the aneurysm wall of human aortas [17].…”

“…We reported previously that annexin II expression was increased in the aneurysmal vessel wall of human aortas [17]. The relevance of that finding was not clear, since we did not examine annexin II expression in normal aortas or during various stages of aneurysm formation due to a limited number of specimens.…”

“…At first, immunohistochemical staining for annexin II was performed with the use of the EnVision?System (DakoCytomation Denmark A/S, Glostrup, Denmark) as previously described [17]. Furthermore, in order to identify which type of cells contained annexin II in the arterial walls, a-smooth muscle actin (a-SMA) and annexin II, or CD68 and annexin II were double-immunostained in the same tissue sections.…”

Paired maximum inspiratory and expiratory plain chest radiographs for assessment of airflow limitation in chronic obstructive pulmonary disease

“…1A) and the distance between the apex and base of the right lung field on PAF views (Fig. 1B), the digitalized pCR images were analyzed using a computed color image analysis software system (WinRoof ® version 5.0; Mitani Co., Fukui, Japan) [14]. The areas of the PAF and RLL lung fields were polygonally surrounded to include the mediastinal and cardiac shadows, and the inner thoracic and diaphragm shadows were traced by hand using an electronic pen, because the overlapped parts of lung, mediastinums, and heart independently could not recognize in two-dimentional pCR techniques (Fig.…”