Expression of antiapoptotic protein survivin in malignant melanoma

Adamkov, Marián; Lauko, L; Rajčáni, J; Bálentová, Soňa; Rybárová, Silvia; D, Mistuna; Statelová, D

doi:10.2478/s11756-009-0134-3

Cited by 8 publications

(5 citation statements)

References 39 publications

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“…Similarly, a high cytoplasmic to nuclear ratio of survivin was associated with an improved overall survival and, conversely, an increased nuclear to cytoplasmic survivin ratio was correlated with an unfavorable overall survival. Both of our previous studies [16,17] and recent results also demonstrated that nuclear and combined nuclear and cytoplasmic survivin reaction are associated with worse prognostic parameters, it is not a case of solely cytoplasmic positivity. It follows that different subcellular survivin compartmentalization has distinct functions.…”

Section: Discussionsupporting

confidence: 53%

“…Review of literature shows that the location of survivin expression is still a matter of discussion [13]. As described previously, there are three patterns of survivin immunohistochemical positivity in malignant cells: nuclear, cytoplasmic and combined (nucleus and cytoplasm) [14,15,16,17]. However, the reason for subcellular compartmentalization of survivin in neoplastic tissues is not yet fully understood.…”

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confidence: 99%

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Correlations of survivin expression with clinicomorphological parameters and hormonal receptor status in breast ductal carcinoma

Adamkov

Kajo²,

Výbohová³

et al. 2012

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The antiapoptotic protein survivin is widely expressed in most human cancers, including carcinomas of the breast. It is rarely detected in corresponding normal adult tissues. Therefore, survivin comes into the limelight as a promising diagnostic biomarker and prognostic parameter. Immunohistochemically, we examined the expression of this protein in 126 cases of ductal breast carcinoma to determine the association with clinicomorphological parameters such as age of patients, grade, stage and size of the primary tumor, lymph node metastasis, vascular invasion as well as estrogen and progesterone status. In each section, the subcellular location of survivin antigen, the intensity of staining and the percentage of labeled cells were assessed. Overall, survivin was expressed in 111 cases (88.1%). The statistical analysis revealed a significant correlation between the nuclear location of survivin and tumor grade 3. Furthermore, a significant relation was also found between vascular invasion and nuclear and combined nuclear and cytoplasmic survivin expression, together with a higher intensity of immunoreaction. However, no significant correlations were shown with other clinicomorphological parameters, such as stage and size of the tumor, lymph node metastasis, estrogen and progesterone receptors and age. Our findings revealed that survivin was frequently overexpressed in carcinoma cells, where it was present in different subcellular compartments. The nuclear positivity of survivin or combined nuclear and cytoplasmic expression was shown to be a poor prognostic parameter in ductal breast carcinoma.

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Section: Discussionsupporting

confidence: 53%

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confidence: 99%

Correlations of survivin expression with clinicomorphological parameters and hormonal receptor status in breast ductal carcinoma

Adamkov

Kajo²,

Výbohová³

et al. 2012

neo

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“…It was found to be the fourth most highly expressed transcript in common cancers [6]. Therefore, survivin is currently undergoing intense research as a potential tumor biomarker [6,7,8,9]. In addition, several studies dealt with the prognostic significance of survivin [10,11,12].…”

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confidence: 99%

Survivin: a promising biomarker in breast carcinoma

Adamkov

Halašová²,

Kajo³

et al. 2010

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The antiapoptotic protein survivin can be detected in most types of malignant tumors, but it is rarely expressed in corresponding normal adult tissues. Therefore, survivin appears to represent a promising diagnostic biomarker. We examined survivin expression in 13 cases of normal breast tissue, 38 cases of fibroadenomas and 80 cases of breast carcinomas by immunohistochemical staining using anti-survivin antibody (DAKO, Clone 12C4). In each section, the intensity of staining, percentage of labeled cells, and the subcellular location of survivin antigen were assessed. Survivin was detected in 4/13 cases of normal breast tissue (30.7%), in 28/38 cases of fibroadenomas (73.7%), and in 67/80 cases of carcinomas (83.8%). Normal breast tissue showed cytoplasmic positivity only. In fibroadenomas, 19 cases (50.0%) revealed cytoplasmic reaction, and in 9 cases (23.7%), small foci of cells with combined nuclear and cytoplasmic location were identified. In carcinomas, cytoplasmic staining was found in 12/80 cases (15.0%), nuclear staining in 10/80 cases (12.5%), and combined cytoplasmic and nuclear staining in 45/80 cases (56.3%). Subcellular location of survivin between benign and malignant lesions revealed significant differences (p<0,001). Our findings point at practical use of survivin detection. We confirm the importance of nuclear staining of survivin antigen in breast carcinoma, which seems to be a notable diagnostic marker for estimation of the degree of neoplasia.

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“…Contrary, all the patients with pT4a and pT4b tumors had strong local expression in numerous tumor cells, significantly higher than other histological stages. A significant correlation of survivin expression with histologic grade and stage was reported not only in melanoma 21 but also in tumor samples of the patients with endometrial carcinoma 22 , ovarian carcinoma 23 , hepatocellular carcinoma 24 and breast cancer 25,26 .…”

Section: Discussionmentioning

confidence: 95%