Expression of antioxidant defense genes in mung bean (Vigna radiata L.) roots under water-logging is associated with hypoxia tolerance

Abstract

This study was conducted to examine the extent of oxidative stress and the role of antioxidant enzymes on hypoxia tolerance in highly tolerant wild species Vigna luteola, and mung bean (Vigna radiata) cvs. T 44 (tolerant) and Pusa Baisakhi (susceptible). contents. Hypoxia induced increase in superoxide dismutase, ascorbate peroxidase, and glutathione reductase activities were higher in T 44 and V. luteola compared with Pusa Baisakhi; and the increases in T 44 and V. luteola continued up to 8th day of water-logging, while in case of Pusa Baisakhi, the maximum increase was observed only on the 2nd day of water-logging. Gene expression studies showed enhanced expression of cytosolic-Cu/Zn-superoxide dismutase (SOD) and cytosolic-ascorbate peroxidase (APX) in the roots of waterlogged V. luteola and T 44, while little expression was observed in control or treated

Changes of antioxidant enzymes of mung bean [Vigna radiata (L.) R. Wilczek] in response to host and non-host bacterial pathogens

“…Primers APX-F (5'CTCAGAGGYTT-MATCGCTGAGAAG3') and APX-R (5'CAGCAAACCC-AAGCTCRGARAGC3'), Cu/Zn-SOD-F (5'ATGGTKAAG-GCTGTGGCAGTTC3') and Cu/Zn-SOD-R (5'ACCTTT-CCCAAGATCATCAGGAGGATC3') (Sairam et al 2011) were used for APX and Cu/Zn-SOD, respectively. Also, β-tubulin with corresponding primers Tubulin-F (5'CTT-GACTGCATCTGCTATGTTCAG3') and Tubulin-R (5'CC-AGCTAATGCTCGGCATACTG3') (Sairam et al 2011) was considered as the house-keeping gene. Total reaction volume was 25 µl and included 10 µl (2X) SYBR green master mix, 100 ng of cDNA, 0.5 µl of 10 µM of each forward and reverse primers, and volume adjusted with water.…”

Water submersion of seeds from three bean cultivars

“…Antioxidants are an important defence to stressors including low oxygen environments. In 25-day-old Vigna, hypoxia induced an increase in the SOD activity, the APX and the GSR in tolerant genotypes (Sairam et al, 2011). In rice coleoptiles under aerobic or anaerobic conditions, the ROS-scavenging enzymes GST, APX cytosolic and putative GST were inhibited (Sadiq et al, 2011), and this response was interpreted as a form of signalling in response to the increasing ROS concentration in anoxic environments (Fukao & Bailey-Serres, 2004).…”

“…Bailey-Serres et al, 2012). Hypoxia over a period of eight days increased the activities of the ROS-scavenging enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GSR) activities in tolerant genotypes of 25-day-old Vigna, suggesting the removal of excess ROS associated with flooding stress (Sairam et al, 2011). Furthermore, the ROS-scavenger enzymes glutathione-S-transferase (GST), cytosolic APX and a putative GST were inhibited in rice coleoptiles under anaerobic conditions (Sadiq et al, 2011).…”

“…Anoxia has been related to protein synthesis inhibition, decrease in the ADH activity in the availability of ATP (Kato-Noguchi, 2001), the activation of pyruvate dehydrogenase, HSPs (heat shock proteins), CBl5 (calcineurin B-like-protein) and USP (universal stress protein) (Sadiq et al, 2011) and further increases in antioxidant enzyme activity during and after the stress release caused by flooding (Sairam et al, 2011). Therefore, biochemical components were analysed in the bean seeds and examined in relation to their performance under submersion.…”

Comparative proteomic analysis of gamma-aminobutyric acid responses in hypoxia-treated and untreated melon roots

“…Excess accumulation of reactive oxygen species (ROS) is a common occurrence during various stress situations, including oxidative stress and hypoxia (Sairam et al, 2011). Higher-order plants have evolved active oxygen-scavenging systems, including several antioxidants and antioxidative enzymes, which can maintain steady-state concentrations of ROS (Parida and Das, 2005).…”