Expression of AP‐2δ in the developing chick retina

Li, Xiaodong; Glubrecht, Darryl; Mita, Raja; Godbout, Roseline

doi:10.1002/dvdy.21744

Cited by 12 publications

(33 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies in mouse and chick have indicated that four of the five known AP-2 family members (AP-2α, AP-2β, AP-2γ, and AP-2δ) are expressed in the developing retina, and that three of these (AP-2α, AP-2β, and AP-2γ) are present in INL cell types while AP-2δ is confined to RGCs (Bisgrove and Godbout, 1999; Zhao et al, 2003; Bassett et al, 2007; Trimarchi et al, 2007; Li et al, 2008). Here, we clarified the expression patterns of AP-2α and AP-2β in horizontal cells of the mouse retina (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…Tcfap2c appeared to be expressed in a pattern similar to Tcfap2a and Tcfap2b , whereas Tcfap2d , the most divergent AP-2 family member, was largely confined to the GCL (Bassett et al, 2007). Likewise, in the developing chick retina, AP-2δ is expressed in a subset of ganglion cells and shows no overlap with AP-2α and AP-2β expression (Bisgrove and Godbout, 1999; Li et al, 2008). …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Overlapping expression patterns and redundant roles for AP‐2 transcription factors in the developing mammalian retina

Bassett

Korol

Deschamps

et al. 2012

Developmental Dynamics

View full text Add to dashboard Cite

Background We have previously shown that the transcription factor AP-2α (Tcfap2a) is expressed in postmitotic developing amacrine cells in the mouse retina. Although retina-specific deletion of Tcfap2a did not affect retinogenesis, two other family members, AP-2β and AP-2γ, showed expression patterns similar to AP-2α. Results Here we show that, in addition to their highly overlapping expression patterns in amacrine cells, AP-2α and AP-2β are also co-expressed in developing horizontal cells. AP-2γ expression is restricted to amacrine cells, in a subset that is partially distinct from the AP-2α/β-immunopositive population. To address possible redundant roles for AP-2α and AP-2β during retinogenesis, Tcfap2a/b-deficient retinas were examined. These double mutants showed a striking loss of horizontal cells and an altered staining pattern in amacrine cells that were not detected upon deletion of either family member alone. Conclusions These studies have uncovered critical roles for AP-2 activity in retinogenesis, delineating the overlapping expression patterns of Tcfap2a, Tcfap2b, and Tcfap2c in the neural retina, and revealing a redundant requirement for Tcfap2a and Tcfap2b in horizontal and amacrine cell development.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overlapping expression patterns and redundant roles for AP‐2 transcription factors in the developing mammalian retina

Bassett

Korol

Deschamps

et al. 2012

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…Immunohistochemistry and immunofluorescence analysis. For immunohistochemistry, chick embryos or eyes were collected at specific developmental stages, fixed in formalin, and embedded in paraffin, as previously described (47). For immunofluorescence analysis, eyes were fixed in 4% paraformaldehyde, cryoprotected in a gradient of sucrose (12%, 16%, and 18%), and embedded in OCT. Antigen retrieval was done by microwaving in 0.01 M citrate (pH 6.0) for 20 min followed by blocking in 500 mM glycine.…”

mentioning

confidence: 99%

The Early Isoform of Disabled-1 Functions Independently of Reelin-Mediated Tyrosine Phosphorylation in Chick Retina

Gao

Monckton

Glubrecht

et al. 2010

Molecular and Cellular Biology

Self Cite

View full text Add to dashboard Cite

The Reelin-Disabled-1 (Dab1) signaling pathway plays a key role in the positioning of neurons during brain development. Two alternatively spliced Dab1 isoforms have been identified in chick retina and brain: Dab1-E, expressed at early stages of development, and Dab1-L (commonly referred to as Dab1), expressed at later developmental stages. The well-studied Dab1-L serves as an adaptor protein linking Reelin signal to its downstream effectors; however, nothing is known regarding the role of Dab1-E. Here we show that Dab1-E is primarily expressed in proliferating retinal progenitor cells whereas Dab1-L is found exclusively in differentiated neuronal cells. In contrast to Dab1-L, which is tyrosine phosphorylated upon Reelin stimulation, Dab1-E is not tyrosine phosphorylated and may function independently of Reelin. Knockdown of Dab1-E in chick retina results in a significant reduction in the number of proliferating cells and promotes ganglion cell differentiation. Our results demonstrate a role for Dab1-E in the maintenance of the retinal progenitor pool and determination of cell fate.

show abstract

“…TFAP2A and TFAP2B are expressed in the amacrine cells of the developing murine and chick retina, with TFAP2B also found in the horizontal cells of chick retina (Bisgrove and Godbout, 1999;Bassett et al, 2007). TFAP2D is expressed in a subset of ganglion cells, whereas TFAP2C and TFAP2E have a poorly defined expression pattern in the retina (Bassett et al, 2007;Li et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

AP2 transcription factor induces apoptosis in retinoblastoma cells

Glubrecht

Godbout

2010

Genes Chromosomes & Cancer

Self Cite

View full text Add to dashboard Cite

The underlying cause of human retinoblastoma is complete inactivation of both copies of the RB1 gene. Other chromosome abnormalities, with the most common being extra copies of chromosome arm 6p, are also observed in retinoblastoma. The RB protein has previously been shown to interact with TFAP2 transcription factors. Here, we show that TFAP2A and TFAP2B, which map to chromosome arm 6p, are expressed in the amacrine and horizontal cells of human retina. TFAP2ARNA can readily be detected in retinoblastoma cell lines and tumors; however, the great majority of retinoblastoma cell lines and tumors are completely devoid of TFAP2A protein and TFAP2B RNA/protein. Transfection of TFAP2A and TFAP2B expression constructs into retinoblastoma cells induces apoptosis and inhibits proliferation. Our results suggest that a consequence of loss of RB1 gene function in retinoblastoma cells is inactivation of TFAP2A and TFAP2B function. We propose that inability to differentiate along the amacrine/horizontal cell lineages may underlie retinoblastoma tumor formation.

show abstract

Expression of AP‐2δ in the developing chick retina

Cited by 12 publications

References 44 publications

Overlapping expression patterns and redundant roles for AP‐2 transcription factors in the developing mammalian retina

Overlapping expression patterns and redundant roles for AP‐2 transcription factors in the developing mammalian retina

The Early Isoform of Disabled-1 Functions Independently of Reelin-Mediated Tyrosine Phosphorylation in Chick Retina

AP2 transcription factor induces apoptosis in retinoblastoma cells

Contact Info

Product

Resources

About