Expression of apelin and apelin receptor (APJ) in porcine ovarian follicles and in vitro effect of apelin on steroidogenesis and proliferation through APJ activation and different signaling pathways

Rak, Agnieszka; Drwal, Eliza; Rame, Ch.; Knapczyk-Stwora, Katarzyna; Słomczyńska, Maria; Dupont, Joëlle; Gregoraszczuk, Ewa Ł.

doi:10.1016/j.theriogenology.2017.04.014

Cited by 70 publications

(74 citation statements)

References 43 publications

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“…Immunohistochemistry revealed the positive higher staining for apelin in membranes of GC, than TC [45]. Apelin was found to increase secretion and 3βHSD and CYP19 expression in co-culture of GC and TC as well as cell proliferation, while decrease IGF1-and FSH-induced steroidogenesis [45]. As a molecular mechanism of these observation, authors showed that AMPKα was involved in the action of apelin on P4 production but MAPK/ERK, AMPKα and Akt/PI3 mediated the proliferative effect of apelin [45].…”

Section: Apelinmentioning

confidence: 92%

“…Apelin was found to increase secretion and 3βHSD and CYP19 expression in co-culture of GC and TC as well as cell proliferation, while decrease IGF1-and FSH-induced steroidogenesis [45]. As a molecular mechanism of these observation, authors showed that AMPKα was involved in the action of apelin on P4 production but MAPK/ERK, AMPKα and Akt/PI3 mediated the proliferative effect of apelin [45]. In an in vitro experiment involving CL cells, apelin also increased P4 secretion by the activity of 3βHSD [46].…”

Section: Apelinmentioning

confidence: 93%

“…In porcine, apelin concentration in the follicular fluid and its expression increases with ovarian follicular growth. Immunohistochemistry revealed the positive higher staining for apelin in membranes of GC, than TC [45]. Apelin was found to increase secretion and 3βHSD and CYP19 expression in co-culture of GC and TC as well as cell proliferation, while decrease IGF1-and FSH-induced steroidogenesis [45].…”

Section: Apelinmentioning

confidence: 94%

See 2 more Smart Citations

The Adipokines in Domestic Animal Reproduction: Expression and Role in the Regulation of Ovarian Function

Kurowska¹,

Mlyczyńska²,

Barbe³

et al. 2018

New Insights Into Theriogenology

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Currently, it is clear that female reproduction is regulated by the hypothalamic-pituitaryovary axis, which produces many hormones that control reproductive stages. It is therefore important to have knowledge of new regulators/hormones controlling reproduction in domestic animals. In female animals, energy metabolism and fertility are tightly connected, and reciprocally regulated. The adipose tissue is well known to be implicated in the secretion of several hormones, such as the adiponectin, resistin, chemerin, visfatin, vaspin and apelin, the so-called adipokines or "adipose tissue-derived hormones". Many reports indicate that adipokines regulate the ovarian follicles' development, the onset of puberty and/or ovulation. This chapter summarizes that several adipokines are expressed in the ovary and that they can regulate ovarian physiology such as the steroid hormone production, cell proliferation, apoptosis and oocyte maturation in different domestic animals like pigs, cows, goats, ewes, chickens and turkeys.

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Section: Apelinmentioning

confidence: 92%

Section: Apelinmentioning

confidence: 93%

Section: Apelinmentioning

confidence: 94%

See 1 more Smart Citation

The Adipokines in Domestic Animal Reproduction: Expression and Role in the Regulation of Ovarian Function

Kurowska¹,

Mlyczyńska²,

Barbe³

et al. 2018

New Insights Into Theriogenology

View full text Add to dashboard Cite

show abstract

“…Apelin and APJ immunoexpression have been reported in granulosa and theca cells, as well as in oocytes in human follicles, at different stages of development [70]. Furthermore, apelin promotes ovarian steroid hormone secretion, in particular, P 4 , and cell proliferation in pigs [71] and E 2 synthesis in humans [70], indicating that apelin has a direct role in folliculogenesis. A recent in vitro study showed that apelin stimulates rat granulosa cell proliferation; however, apelin inhibits granulosa cell apoptosis via PI3K/AKT signalling [72].…”

Section: Indirect Actions Of Bpa In the Ovary Through Adipokinesmentioning

confidence: 98%

The Ovary as a Target Organ for Bisphenol A Toxicity

Ptak¹,

Hoffmann²,

Rak³

2017

Bisphenol a Exposure and Health Risks

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The ovary is a hormone-sensitive organ that produces steroid hormones. Recent studies show that bisphenol A (BPA) can affect female reproduction; thus, it is important to identify the possible toxic effects of BPA on the ovary because this organ is indispensable for fertility. This chapter summarises the effects of BPA on the ovary by describing how they directly affect folliculogenesis, steroidogenesis and receptor signalling and how they indirectly affect the expression of adipokines and/or their receptors, which exert endocrine or autocrine functions within the ovary.

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“…JEG-3 or BeWo cells (4x10 3 cells/per well of 96-well plate) were cultured in dMEM/F12 with 10% FBS for 24 h. Next, media were replaced by dMEM/F12 with 1% FBS and cells were treated for 24, 48 and 72 h with apelin at doses 0.02, 0.2, 2.0, 20 and 200 ng/ml. The concentrations of apelin were chosen based on the authors' preliminary research and previous study (33). The alamarBlue stock solution or cell counting Kit-8 (ccK-8) reagent in amounts equal to 10% of the incubation volume was added, according to the manufacturers' protocol.…”

Section: Treatmentmentioning

confidence: 99%

Apelin and apelin receptor in human placenta: Expression, signalling pathway and regulation of trophoblast JEG‑3 and BeWo cells proliferation and cell cycle

et al. 2020

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Placentation requires the production of numerous growth factors, hormones and transcription factors. Many of them, like the adipose tissue-derived leptin or adiponectin, have been identified in the placenta and their role has been established in the proliferation and subsequent development of the placenta. Apelin is another adipokine known for proliferative effects in different cell types. PcR, immunoblotting and immunocytochemistry were used to study mRNA and protein expression of apelin and its receptor (APJ) in syncytiotrophoblast (BeWo) and cytotrophoblast (JEG-3) cells as well in immunohistochemistry in human normal placenta slides. The effect of apelin on cell proliferation study was investigated by alamarBlue ® and cell counting Kit-8 assays, the cell cycle by the flow cytometry method and the protein expression of cyclins and phosphorylation level of extracellular signal-regulated kinases (ERK)1/2, phosphatidylinositol 3'-kinase/protein kinase B (Akt), signal transducer and activator of transcription 3 (Stat3) and 5'-monophosphate-activated protein kinase (AMPKα) were studied by western blotting. Apelin was increased in JEG-3 compared with in BeWo cells, while APJ was the same in both placenta cell lines. Immunocytochemical analyses revealed high cytoplasmic and/or membrane apelin localisation in JEG-3, while BeWo cells exhibited markedly weaker apelin signal in the cytoplasm. Apelin increased cell proliferation as well as the percentage of cells in the G2/M phase of the cell cycle, cyclin proteins and the expression of all kinases mentioned above. In conclusion, apelin by promotion of trophoblast cell proliferation by APJ and ERK1/2, Stat3 and AMPKα signalling could be a new important adipokine in the regulation of early placental development.

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Expression of apelin and apelin receptor (APJ) in porcine ovarian follicles and in vitro effect of apelin on steroidogenesis and proliferation through APJ activation and different signaling pathways

Cited by 70 publications

References 43 publications

The Adipokines in Domestic Animal Reproduction: Expression and Role in the Regulation of Ovarian Function

The Adipokines in Domestic Animal Reproduction: Expression and Role in the Regulation of Ovarian Function

The Ovary as a Target Organ for Bisphenol A Toxicity

Apelin and apelin receptor in human placenta: Expression, signalling pathway and regulation of trophoblast JEG‑3 and BeWo cells proliferation and cell cycle

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