Expression of apoptosis-inducing factor (AIF) in the aged rat brain

Yu, Wenbin; Gubkina, Olena; Mechawar, Naguib; Elwell, Diana; Quirion, Rémi; Krantic, Slavica

doi:10.1016/j.neurobiolaging.2009.01.010

Cited by 3 publications

(6 citation statements)

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“…Therefore, the differential sensitivity of mitochondrial ATP production and increased ROS between these two regions reported herein may be associated with their respective level of expression of AIF. In agreement with this hypothesis, our previous study revealed significant differences in the level of AIF expression between brain regions of the rodent brain [33]. Furthermore, low level of AIF expression in Harlequin mice has been linked to mitochondrial respiratory chain dysfunction [24] and AIF has been implicated in ROS production [61].…”

Section: Discussionsupporting

confidence: 60%

“…These preliminary experiments (data not shown) allowed us to determine the following experimental conditions: 20 μg of proteins per assay, 40 min incubation at 37°C and then addition of 50 μM H 2 O 2, followed by 5 min incubation at room temperature. The brain region homogenates used for ROS quantification were prepared in RIPA buffer as previously reported [33]. The fluorescence was quantified at the excitation/emission wavelength of 485/530 nm (Synergy 4, Biotek, Winooski, VT, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Protein extracts were prepared by using RIPA buffer extraction as previously reported [33]. Briefly, 40 μg of total proteins, diluted in Laemli buffer (Sigma-Aldrich, Oakville, Ontario, Canada), were boiled for 5 min and then separated by SDS-PAGE, on a Tris-Glycine 4-20% gradient gel (Invitrogen, Burlington, ON, Canada), at 130 V for 2 ½ h. Proteins were transferred to nitrocellulose membranes at 80 V for 45 min at 4°C, under agitation.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, although AIF is ubiquitously expressed in the rodent brain, we observed a brain region-specific gradient of distribution in the normal rat brain [33]. Such contrasting levels of AIF expression indicated that AIF-induced PCD may be region specific.…”

Section: Introductionmentioning

confidence: 94%

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The expression of apoptosis inducing factor (AIF) is associated with aging-related cell death in the cortex but not in the hippocampus in the TgCRND8 mouse model of Alzheimer’s disease

Bonnet

Farso

et al. 2014

BMC Neurosci

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BackgroundRecent evidence has suggested that Alzheimer’s disease (AD)-associated neuronal loss may occur via the caspase-independent route of programmed cell death (PCD) in addition to caspase-dependent mechanisms. However, the brain region specificity of caspase-independent PCD in AD-associated neurodegeneration is unknown. We therefore used the transgenic CRND8 (TgCRND8) AD mouse model to explore whether the apoptosis inducing factor (AIF), a key mediator of caspase-independent PCD, contributes to cell loss in selected brain regions in the course of aging.ResultsIncreased expression of truncated AIF (tAIF), which is directly responsible for cell death induction, was observed at both 4- and 6-months of age in the cortex. Concomitant with the up-regulation of tAIF was an increase in the nuclear translocation of this protein. Heightened tAIF expression or translocation was not observed in the hippocampus or cerebellum, which were used as AD-vulnerable and relatively AD-spared regions, respectively. The cortical alterations in tAIF levels were accompanied by increased Bax expression and mitochondrial translocation. This effect was preceded by a significant reduction in ATP content and an increase in reactive oxygen species (ROS) production, detectable at 2 months of age despite negligible amounts of amyloid-beta peptides (Aβ).ConclusionsTaken together, these data suggest that AIF is likely to play a region-specific role in AD-related caspase-independent PCD, which is consistent with aging-associated mitochondrial impairment and oxidative stress.

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Section: Discussionsupporting

confidence: 60%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

See 2 more Smart Citations

The expression of apoptosis inducing factor (AIF) is associated with aging-related cell death in the cortex but not in the hippocampus in the TgCRND8 mouse model of Alzheimer’s disease

Bonnet

Farso

et al. 2014

BMC Neurosci

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“…The main nuclear actions of calpain-activated AIF are associated with chromatin condensation and large scale DNA fragmentation, albeit AIF does not display any intrinsic endonuclease activity. All three AIF forms (precursor 67 kDa, mitochondrial membrane-embedded 62 kDa and mitochondrialreleased 57 kDa) are detected in primary cultures of rat hippocampus (our unpublished data), in rats [41,42], and postmortem human brain tissues (cortex and hippocampus) obtained from AD and control subjects [7]. It has been recently shown that AIF nuclear translocation is increased and colocalized with neurofibrillary tangles -one of the main pathological features in AD-in the hippocampus and entorhinal cortex -in the post-mortem of AD brains [7].…”

Section: The Role Of Caspases and Aif In Alzheimer's Diseasementioning

confidence: 69%

Possible Involvement of Programmed Cell Death Pathways in the Neuroprotective Action of Polyphenols

Bastianetto¹,

Krantic²,

Chabot³

et al. 2011

CAR

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One of the hallmarks of Alzheimer's disease is the accumulation of senile plaques composed of extra-cellular aggregates of beta-amyloid (Aβ) peptides. It is well established that at least in vitro, Aβ triggers apoptotic cell death via the activation of caspase-dependent and -independent cell death effectors, namely caspase-3 and apoptosis inducing factor (AIF), respectively. Epidemiological studies have reported that elderly people have a lower risk (up to 50%) of developing dementia if they regularly eat fruits and vegetables and drink tea and red wine (in moderation). Numerous studies indicate that polyphenols derived from these foods and beverages account for the observed neuroprotective effects. In particular, we have reported that polyphenols extracted from green tea (i.e. epigallocatechin gallate or EGCG) and red wine (i.e. resveratrol) block Aβ-induced hippocampal cell death, by at least partially inhibiting Aβ fibrillisation. It has been shown that polyphenols may also modulate caspase-dependent and -independent programmed cell death (PCD) pathways. Indeed, polyphenols including resveratrol, EGCG and luteolin significantly inhibit the activation of the key apoptotic executioner, caspase-3 and are able to modulate mitogen-activated protein kinases known to play an important role in neuronal apoptosis. Moreover, it has been reported that polyphenols may exert their anti-apoptotic action by inhibiting AIF release from mitochondria, thus providing new mechanism of action for polyphenols. This review aims to update the current knowledge regarding the differential effects of polyphenols on PCD pathways and discuss their putative neuroprotective action resulting from their capacity to modulate these pathways.

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Deletion of PaAif2 and PaAmid2, two genes encoding mitochondrial AIF-like oxidoreductases of Podospora anserina, leads to increased stress tolerance and lifespan extension

2010

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Wild-type strains of the ascomycete Podospora anserina are characterized by a limited lifespan. Mitochondria play a central role in this ageing process raising the question of whether apoptosis-like processes, which are also connected to mitochondrial function, are involved in the control of the final stage in the fungal life cycle. While a role of two metacaspases in apoptosis and lifespan control was recently demonstrated in P. anserina, virtually nothing is known about the function of the protein family of apoptosis-inducing factors (AIFs). Here we report data about proteins belonging to this family. We demonstrate that the cytosolic members PaAIF1 and PaAMID1 do not affect lifespan. In contrast, loss of PaAIF2 and PaAMID2, which both were localized to mitochondria, are characterized by a significantly increased ROS tolerance and a prolonged lifespan. In addition, deletion of PaAmid2 severely affects sporogenesis. These data identify components of a caspase-independent molecular pathway to be involved in developmental processes and in the induction of programmed cell death in the senescent stage of P. anserina.

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Expression of apoptosis-inducing factor (AIF) in the aged rat brain

Cited by 3 publications

References 3 publications

The expression of apoptosis inducing factor (AIF) is associated with aging-related cell death in the cortex but not in the hippocampus in the TgCRND8 mouse model of Alzheimer’s disease

The expression of apoptosis inducing factor (AIF) is associated with aging-related cell death in the cortex but not in the hippocampus in the TgCRND8 mouse model of Alzheimer’s disease

Possible Involvement of Programmed Cell Death Pathways in the Neuroprotective Action of Polyphenols

Deletion of PaAif2 and PaAmid2, two genes encoding mitochondrial AIF-like oxidoreductases of Podospora anserina, leads to increased stress tolerance and lifespan extension

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