Expression of apoptosis-related genes in the organ of Corti, modiolus and stria vascularis of newborn rats

Gross, Johann; Machulik, Astrid; Amarjargal, Nyamaa; Moller, Renate; Ungethüm, Ute; Kuban, Ralf‐Jürgen; Fuchs, Frank-Uwe; Андреева, Н. И.; Fuchs, Julia; Henke, Wolfgang; Pohl, Elena E.; Szczepek, Agnieszka J.; Haupt, H.; Mazurek, Birgit

doi:10.1016/j.brainres.2007.05.061

Cited by 21 publications

(18 citation statements)

References 75 publications

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“…Details of the preparation of the fragments, the culture conditions and the testing of the viability of the explants were reported previously (Gross et al, 2007). Briefly, the fragments were incubated in four-well tissue culture dishes in Dulbecco`s Modified Eagle Medium/F12Nutrient (1:1) Mixtures (Gibco, Karlsruhe, Germany) supplemented with 10 % fetal bovine serum.…”

Section: Explant Culturesmentioning

confidence: 99%

“…Altogether, 16 RNA preparations arising from three independent series were analyzed within one year: four samples from freshly prepared tissue (OC1, OC2, MOD, SV) and 12 experimental samples from cultures of OC, MOD and SV under normoxic (n = 2) and hypoxic conditions (n = 2). Further details of the RNA isolation and quantification and the cDNA microarray analysis were previously reported (Gross et al, 2007).…”

Section: Cdna Microarray Analysismentioning

confidence: 99%

“…Organotypic cultures of the SV, the OC and the MOD were used to experimentally study the differential gene expression of these regions to injury stress and hypoxia (Gross et al, 2007). In freshly prepared tissue, about 2-10 % of all nuclei were found to be stained by propidium iodide, indicating cell damage during preparation of the cochlear tissue.…”

Section: Introductionmentioning

confidence: 99%

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Gene Expression Patterns in Functionally Different Cochlear Compartments of the Newborn Rat

Gross¹,

Mazurek²

2015

JMBR

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In an experimental model of organotypic cultures of the stria vascularis (SV), the organ of Corti (OC) and the modiolus (MOD), we compared the expression levels and injury/hypoxia induced response of 36 genes associated with the cells´ energy-producing and energy-consuming processes, using the microarray technique. A decrease of expression was observed for most of the voltage-dependent K + -and Ca ++ -channels as an effective mechanism to lower energetic demands. We identified two gene networks of transcripts that are differentially expressed across the three regions. One cluster is associated with the transcription factor hypoxia-inducing factor (Hif-1a) and the second one with the caspase and calpain cell death genes Casp3, Capn1, Capn2 and Capns1. The Hif-1a gene subset consists of genes belonging to the glucose metabolism (glucose transporter Slc2a1, glycolytic enzymes Gapdh, Hk1 and Eno2), the Na + /K + homeostasis (ATPase Atp1a1) and the glutamate pathway (NMDA receptor associated protein 1 Grina, glutamate transporter Slc1a1, Slc1a3). The Slc2a1, Gapdh, Hk1, Slc1a3, Grina and Atp1a1 transcripts are also members of the cell death subset indicating a role they have to play in the differential regional cell death rates. The newly identified genes Grina and calnexin (Canx) may play specific and yet unknown roles in regulating cell death induced by injury and hypoxia in the inner ear. We assume that the differential regional response occurs on the basis of endogenous gene regulatory mechanisms and may be important to maintaining the cochlea's function following damage from trauma and hypoxia.

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Section: Explant Culturesmentioning

confidence: 99%

Section: Cdna Microarray Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gene Expression Patterns in Functionally Different Cochlear Compartments of the Newborn Rat

Gross¹,

Mazurek²

2015

JMBR

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“…Previously, we had introduced organotypic cultures of the SV, the OC and the MOD of newborn rats as an experimental model to study the differential response of neurobiological genes to injury stress and hypoxia [3] . Analysis of gene expression showed that two basic pathogenetic mechanisms are involved in this experimental model: mechanically induced inflammation and hypoxia [4] .…”

Section: Research Articlementioning

confidence: 99%

“…Details of the preparation and culturing were described previously [3] . Briefly, the membranous cochleae from 3-5 day-old Wistar rats were dissected into the OC, MOD and SV regions and exposed to normoxic and hypoxic conditions for 24 h. For culture, the cochlear parts were incubated in 4-well tissue culture dishes.…”

Section: Explant Culturesmentioning

confidence: 99%

Expression of nestin-associated genes in the inner ear of newborn rats following injury and hypoxia

Gross

Mazurek

2015

Inflamm Cell Signal

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We used organotypic cultures of the stria vascularis (SV), the organ of Corti (OC) and the modiolus (MOD) of newborn rats to analyze the differential expression levels and responses of cytoskeletal, myelin-and neural growth factor-associated genes following preparatory injury and hypoxia. The transcript mRNA levels of the neurofilaments Nefl, Nefm, the microtubule-associated proteins Mapt, Map1a, Map2, and of the myelin basic protein Mbp decrease during 24 h in a coordinated way across the MOD and OC regions. The increased cell death rate in the MOD region is associated with an increased expression of Nes, the transcript encoding the intermediate filament nestin, and of the neural growth factor receptor Ngfr, the growth-associated protein 43 Gap43 and the Purkinje cell protein Pcp4. The correlation analysis revealed close associations between Nes expression and genes involved in apoptotic and necrotic cell death (caspase Casp3, calpains Capn1, Capn2, Capns1), the glutamate pathway (glutamate receptor NMDA associated protein 1 Grina, glial high affinity glutamate transporter Slc1a3), cytoskeletal genes (Nefm, Map2, Map1a, Mbp), transcription factors (hypoxia inducible factor Hif-1a, jun proto-oncogene Jun, brain expressed myelocytomatosis B-myc, HES family bHLH transcription factor 1 Hes-1, forkhead-box D3 Foxd3) and neural growth factors (Ngfr, Gap43 and Pcp4). The unique response and the composition of the Nes cluster made us conclude that the expression of cell-death-associated genes and the regeneration-associated genes takes place in a coordinated way, and differs between the MOD and the OC/SV regions.

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Ohr und Hören

Koch

2016

HNO Fragen Und Antworten

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Expression of apoptosis-related genes in the organ of Corti, modiolus and stria vascularis of newborn rats

Cited by 21 publications

References 75 publications

Gene Expression Patterns in Functionally Different Cochlear Compartments of the Newborn Rat

Gene Expression Patterns in Functionally Different Cochlear Compartments of the Newborn Rat

Expression of nestin-associated genes in the inner ear of newborn rats following injury and hypoxia

Ohr und Hören

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