Expression of aquaporin 1 in the pig peri-ovarian vascular complex during the estrous cycle and early pregnancy

Skowroński, Mariusz T.; Frąckowiak, Leszek; Skowrońska, Agnieszka

doi:10.1016/s1642-431x(12)60067-9

Cited by 7 publications

(10 citation statements)

References 38 publications

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“…In sheep, as in other mammals FSH appears necessary for transition of the small antral to larger follicles (McNatty et al 2007). Cyclical variations in the levels of progesterone, estrogens and FSH presumably are associated with the observed changes in follicular development (Stabenfeldt et al 1969, Tillson et al 1970, Henricks et al 1972. The progression of the follicle from the preovulatory to the peri-ovulatory state is initiated by LH in response to the estrogens secreted by the grooving follicle.…”

Section: Discussionmentioning

confidence: 99%

“…Thoroddsen et al (2011) showed the presence of AQP1-4 in isolated human granulosa and theca cells. Our laboratory revealed the expression of AQPs in the reproductive system of gilts (Skowronski 2010, Skowronski et al 2009, 2011a. However, there are no studies concerning localization and quantitative expression of AQPs in the ovary of cycling and early pregnant pigs.…”

Section: Introductionmentioning

confidence: 99%

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Expression of Aquaporin 1, 5 and 9 in the Ovarian Follicles of Cycling and Early Pregnant Pigs

Skowrońska¹,

Młotkowska²,

Eliszewski³

et al. 2015

Physiol Res

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Aquaporins (AQPs) are water channel proteins responsible for water homeostasis and important for proper functioning of all body systems, including reproductive structures. This study was designed to determine their localization and quantitative changes in the pig ovary during different stages of the estrous cycle and early pregnancy. The expression of AQP 1, 5 and 9 proteins was determined by immunocytochemistry and Western blot analyses. AQP1 was found in the plasma membranes of capillary endothelium, AQP5 – in the plasma membranes of granulosa cells of developing follicles and flattened follicle cells of the primordial follicles, and AQP9 – in granulosa cells of the developing follicles. In the cyclic pigs, the expression of AQP1 and 5 proteins was the highest on Days 18-20, but did not change significantly between Days 2-4, 10-12 and 14-16 of the cycle. In pregnant pigs (Days 14-16 and 30-32), the expression of AQP1 and 5 did not change and was similar to that observed during Days 10-12 and 14-16. In turn, AQP9 expression did not change between all studied periods. In conclusion, studied AQP are localized in different cells populations, the endothelial and granulosa cells, and AQP1 and 5 seem to be crucial for follicular development in pigs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of Aquaporin 1, 5 and 9 in the Ovarian Follicles of Cycling and Early Pregnant Pigs

Skowrońska¹,

Młotkowska²,

Eliszewski³

et al. 2015

Physiol Res

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“…For example, in cyclic gilts, the abundance of AQP 1, 5, and 9 proteins in the uterus do not appear to differ between Days 10-12 and Days 14-16 of the estrous cycle, but may increase on Days 2-4 and 18-20 of the estrous cycle (68). During the estrous cycle and early pregnancy, AQP1 is expressed in endothelial cells of the pig peri-ovarian vascular complex (70). Additionally, our results indicate that at least 9 AQP (AQP 1, 3, 4, 5, 6, 7, 8, 9, and 11) are expressed in the placentae of gilts on Day 25 of gestation ( Figure 1A).…”

Section: Pigsmentioning

confidence: 98%

“…There are reports of expression of AQP 1, 5, and 9 in the uterus (68) and oviduct (69) There is evidence that, in pigs, AQP1 is localized within blood vessels of the uterus and oviduct, AQP5 in both smooth muscle cells and epithelial cells of the uterus and oviduct, and AQP9 in epithelial cells of the uterus and oviduct (68,69). However, AQP 1, 5, and 9 exhibit distinct patterns of expression during different phases of the estrous cycle and early pregnancy (68, 69), suggesting possible cell-specific regulation of their gene expression by reproductive hormones (70). For example, in cyclic gilts, the abundance of AQP 1, 5, and 9 proteins in the uterus do not appear to differ between Days 10-12 and Days 14-16 of the estrous cycle, but may increase on Days 2-4 and 18-20 of the estrous cycle (68).…”

Section: Pigsmentioning

confidence: 99%

Aquaporins in the female reproductive system of mammals

et al. 2015

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Water and ion accumulation is the driving force for rapid expansion of the amnion and allantois of mammalian placentae during early gestation, and, therefore, essential for embryonic/fetal growth and survival. Aquaporins (AQP) are a family of small integral plasma membrane proteins that primarily transport water across the plasma membrane. To date, thirteen AQP isoforms (AQP 0-12) have been identified in mammals. AQP 1, 2, 3, 4, 5, 6, 7, 8, 9, and 11 are expressed in the female reproductive tract. Based on their structural and functional properties, AQPs are divided into three subgroups: classical aquaporins (AQP 0, 1, 2, 4, 5, 6, and 8), aquaglyceroporins (AQP 3, 7, 9, and 10), and superaquaporins (AQP 11 and 12). Expression of AQPs in the uterus and placenta is regulated by hormones and nutrients to maintain fluid homeostasis in the conceptus. The underlying mechanisms may involve signal transduction pathways mediated by cAMP, MAPK, PKC, and PI3K/Akt/mTOR. Such new knowledge will advance basic understanding of mammalian reproductive biology to enhance embryonic/fetal survival, growth and development in women and livestock.

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“…We previously found an association between accumulation of uterine cavity fluid and impaired IVF outcome (He et al 2010), but how this rapid luminal fluid transport takes place remains unknown. Discovery of aquaporins (AQPs) and their specific expression and tissue distribution in the mammalian uterus brings new clues in understanding stromal oedema and uterine luminal fluid movement during the process of embryo implantation (Skowronski et al 2011). In humans, both AQP1 and AQP2 are expressed in endometria (He et al 2005;Feng et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Decreased expression of aquaporin 2 is associated with impaired endometrial receptivity in controlled ovarian stimulation

Zhang

et al. 2016

Reprod. Fertil. Dev.

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Recently, there has been evidence of decreased implantation rates with in vitro fertilisation and embryo transfer due to controlled ovarian stimulation (COS). The aim of this study was to investigate the effect of COS on embryo implantation and the role of aquaporin 2 (AQP2). We recruited eight patients who underwent COS and 40 matched controls. Endometrial samples were collected on Day 4~8 after injection of human chorionic gonadotrophin in the COS group and in the mid-secretory phase in the control group. Human endometrial morphological changes after COS were examined and expression of AQP2, leukaemia inhibitory factor (LIF) and integrin B3 (ITGB3) were determined by quantitative polymerase chain reaction, western blotting and immunohistochemistry in human endometrium and Ishikawa cells. Attachment rates were obtained using the embryo attachment test. The results showed that endometrial epithelial cells from the COS group were disrupted and lacked pinopodes. Messenger RNA and protein levels of AQP2, LIF and ITGB3 decreased in endometrial samples from the COS group. Knockdown of AQP2 resulted in reduced expression of LIF and ITGB3 and reduced embryo attachment rates. In conclusion, impaired endometrial receptivity in patients who underwent COS is correlated with a decreased expression of AQP2.

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Expression of aquaporin 1 in the pig peri-ovarian vascular complex during the estrous cycle and early pregnancy

Cited by 7 publications

References 38 publications

Expression of Aquaporin 1, 5 and 9 in the Ovarian Follicles of Cycling and Early Pregnant Pigs

Expression of Aquaporin 1, 5 and 9 in the Ovarian Follicles of Cycling and Early Pregnant Pigs

Aquaporins in the female reproductive system of mammals

Decreased expression of aquaporin 2 is associated with impaired endometrial receptivity in controlled ovarian stimulation

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