Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis.

Szatraj, Katarzyna; Szczepankowska, Agnieszka K.; Sa̧czyńska, Violetta; Florys, Katarzyna; Gromadzka, Beata; Łepek, Krzysztof; Płucienniczak, Grażyna; Szewczyk, Bogusław; Zagórski-Ostoja, Włodzimierz; Bardowski, Jacek

doi:10.18388/abp.2014_1884

Cited by 19 publications

(12 citation statements)

References 26 publications

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“…A positive effect (induction of the specific antibodies and reduction of the level of colonization of mice by H. felis ) was only seen after vaccination with a Lactobacillus strain (Lee et al 2001 ; Corthesy et al 2005 ). It is postulated that some L. lactis strains that reveal high affinity to mucus are promising candidates for development of a vaccine against bird flu (Szatraj et al 2014 ; Radziwill-Bienkowska et al 2014 ).…”

Section: Lab As Carriers Of Heterologous Bacterial Parasitic and VImentioning

confidence: 99%

“…Coproduction induced significantly higher levels of specific IgG antibodies than when L. lactis produced only TTFC (Steidler et al 1995 , 1998a ). The use of L. lactis expressing chicken interleukin 2 (chIL-2) together with avian influenza hemagglutinin (H5) also revealed adjuvant properties of chIL-2, and the study suggests that L. lactis could be a promising candidate as antigen carrier in vaccines against avian flu (Szatraj et al 2014 ). In recent years, the influence of IL-12 on the immune system has been extensively studied.…”

Section: Modulation Of the Activity Of The Immune Systemmentioning

confidence: 99%

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Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination

Wyszyńska

Kobierecka

Bardowski

et al. 2015

Appl Microbiol Biotechnol

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121

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Lactic acid bacteria (LAB) are a diverse group of Gram-positive, nonsporulating, low G + C content bacteria. Many of them have been given generally regarded as safe status. Over the past two decades, intensive genetic and molecular research carried out on LAB, mainly Lactococcus lactis and some species of the Lactobacillus genus, has revealed new, potential biomedical LAB applications, including the use of LAB as adjuvants, immunostimulators, or therapeutic drug delivery systems, or as factories to produce therapeutic molecules. LAB enable immunization via the mucosal route, which increases effectiveness against pathogens that use the mucosa as the major route of entry into the human body. In this review, we concentrate on the encouraging application of Lactococcus and Lactobacillus genera for the development of live mucosal vaccines. First, we present the progress that has recently been made in the field of developing tools for LAB genetic manipulations, which has resulted in the successful expression of many bacterial, parasitic, and viral antigens in LAB strains. Next, we discuss the factors influencing the efficacy of the constructed vaccine prototypes that have been tested in various animal models. Apart from the research focused on an application of live LABs as carriers of foreign antigens, a lot of work has been recently done on the potential usage of nonliving, nonrecombinant L. lactis designated as Gram-positive enhancer matrix (GEM), as a delivery system for mucosal vaccination. The advantages and disadvantages of both strategies are also presented.

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Section: Lab As Carriers Of Heterologous Bacterial Parasitic and VImentioning

confidence: 99%

Section: Modulation Of the Activity Of The Immune Systemmentioning

confidence: 99%

Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination

Wyszyńska

Kobierecka

Bardowski

et al. 2015

Appl Microbiol Biotechnol

Self Cite

121

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“…The mucosal delivery systems include live bacterial vectors and among them those that use commensal bacteria, such as lactobacilli or certain streptococci and staphylococci. We have shown that using Lactococcus as a producer of an influenza virus antigenic protein, haemagglutinin, is a promising approach (Szatraj et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Lactococcus lactis IBB477 presenting adhesive and muco-adhesive properties as a candidate carrier strain for oral vaccination against influenza virus.

et al. 2014

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In the gastrointestinal tract (GIT), adhesion is a prerequisite for bacterial colonization. Lactococci can be used in functional food (probiotics) and health-related applications (mucosal vaccines, therapeutic drug delivery), both potentially involving adhesive properties. A candidate lactic acid bacterium for influenza antigen delivery through the GIT should display the ability to adhere. The present work probes the interactions between Lactococcus lactis and mucins using pig gastric mucin (PGM) as a model. Two strains were used for the optimization of the screening method for adhesion: L. lactis subsp. cremoris IBB477 persistent in the GIT of germ-free rats, and the low-adhering control strain MG1820. High adhesion to bare and mucin-coated polystyrene of IBB477 in comparison with MG1820 was observed. We searched for genetic determinants potentially involved in the adhesion/ muco-adhesion of IBB477, identifying two such genes: prtP and a gene coding for a protein with MUB and MucBP domains. Based on its persistence in the GIT and adhesive properties, L. lactis IBB477 is a candidate carrier strain for expression of influenza haemagglutinin (HA) protein for induction of mucosal immune response.

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“…In our previous work, we have shown that it is possible to engineer L. lactis cells to produce the H5 protein of the avian influenza virus H5N1 [33]. In this study, we aimed at further improvement of the approach.…”

Section: Discussionmentioning

confidence: 99%

RecombinantLactococcus lactisExpressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials

Szczepankowska

Szatraj

Sałański

et al. 2017

BioMed Research International

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Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. Heterologous proteins of different origin have been successfully expressed in various LAB species, including Lactococcus lactis. Recombinant L. lactis strains have been shown to induce specific local and systemic immune responses against various antigens. Our study aimed at constructing a L. lactis strain expressing haemagglutinin of a Polish avian H5H1 influenza isolate and examining its effect on animals. Expression of the cloned H5 gene was achieved using the nisin-controlled gene expression system. Detection of the intracellular H5 antigen produced in L. lactis was performed by Western blot analysis and confirmed using mass spectrometry. The potential of L. lactis recombinant cells to induce an immune response was examined by setting up preliminary immunization trials on chickens and mice. Obtained sera were tested for specific antibodies by ELISA assays. The results of these studies are a promising step toward developing a vaccine against the bird flu using Lactococcus lactis cells as bioreactors for efficient antigen production and delivery to the mucosal surface.

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Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis.

Cited by 19 publications

References 26 publications

Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination

Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination

Lactococcus lactis IBB477 presenting adhesive and muco-adhesive properties as a candidate carrier strain for oral vaccination against influenza virus.

RecombinantLactococcus lactisExpressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials

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