Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm

Lee, Mei-Phing; Yeun, Li-Huey; Abdullah, Rohana

doi:10.2225/vol9-issue2-fulltext-3

Cited by 19 publications

(8 citation statements)

References 28 publications

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“…Furthermore, knowledge of insecticidal proteins elucidates genes that can be harnessed in producing insect‐resistant genetically‐modified crops. A well‐established model is the Bt toxin‐producing crops applied across a wide range of crop types (Lee, Yeun, & Abdullah, ; ISAAA, n.d.). However, the effectiveness of proteomics in providing insight of EPMs’ biology has a caveat.…”

Section: Promises and Caveat Of Proteomics For Bioinsecticide Researchmentioning

confidence: 99%

Proteomics as a tool for tapping potential of entomopathogens as microbial insecticides

Harith-Fadzilah

Abdul-Ghani

Hassan

2018

Arch Insect Biochem Physiol

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Biopesticides are collective pest control harnessing the knowledge of the target pest and its natural enemies that minimize the risks of synthetic pesticides. A subset of biopesticides; bioinsecticides, are specifically used in controlling insect pests. Entomopathogens (EPMs) are micro-organisms sought after as subject for bioinsecticide development.However, lack of understanding of EPM mechanism of toxicity and pathogenicity slowed the progress of bioinsecticide development. Proteomics is a useful tool in elucidating the interaction of entomopathogenic fungi, entomopathogenic bacteria, and entomopathogenic virus with their target host.Collectively, proteomics shed light onto insect host response to EPM infection, mechanism of action of EPM's toxic proteins and secondary metabolites besides characterizing secreted and membrane-bound proteins of EPM that more precisely describe relevant proteins for host recognition and mediating pathogenesis. However, proteomics requires optimized protein extraction methods to maximize the number of proteins for analysis and availability of organism's genome for a more precise protein identification.

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Section: Promises and Caveat Of Proteomics For Bioinsecticide Researchmentioning

confidence: 99%

Proteomics as a tool for tapping potential of entomopathogens as microbial insecticides

Harith-Fadzilah

Abdul-Ghani

Hassan

2018

Arch Insect Biochem Physiol

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“…Regarding to biotic factors, [71] introduced a synthetic cryIA(b) gene into oil palm while biobalistic. When cryIA(b) gene was expressed, it produced proteins which upon crystallization are highly toxic to Lepidoptera among which, Metisa plana is a major insect pest for oil palm.…”

Section: Advances In Seed Biology 304mentioning

confidence: 99%

Genetic Improvement of Oilseed Crops Using Modern Biotechnology

Villanueva-Mejía¹,

Álvarez²

2017

Advances in Seed Biology

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In 2009, big challenges facing the agricultural sector in the twenty-first century were presented to the world. Human population growth, increased life expectancy, loss of biodiversity, climate change and accelerated land degradation are the main factors contributing to rethink agriculture system production. In that scenery, modern biotechnology has set a stage for the advancement of agricultural practices and it is clearly an important ally to apply a broad array of technologies and innovative systems where they are most needed, such as enhancing crop productivity, increasing yields, and ultimately ensuring food security. One of the biggest challenges is related to technify production systems, but with no doubt, developing genetic improvement toward getting an efficient and sustainable agriculture, generating new seed qualities (new traits), such as, among others, to upset fatty acids content in oilseed crops have been growing up significantly due to industry interest. In this study, a review about the main advances in genetic improvement of some oilseed crops, starting with omics to understand metabolic routes and to find out key genes in seed oil production, and also, getting in use of modern biotechnology to alter the production of fatty acids, and to face biotic challenges in oilseed crops is presented.

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“…In mature open flowers, staining was observed in the green bract surrounding the flower, in the green ovary wall and in the stigma, and in pollen, but not in ovules. Lee et al (2006) reported the integration and expression of the cry1A(b) insecticidal gene under rbcs promoter in transformed oil palm. A biolistic method was used to transform immature embryos (IEs) of oil palm.…”

Section: Rubisco Small Subunit Promoter As a Green Tissue-specific Prmentioning

confidence: 99%

Endeavours of RuBisCO small subunit promoter as a tool of green tissue specific expression

Bakhsh¹,

Husnain²

2012

CZECH J GENET PLANT

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Transcriptomics has the potential to rapidly increase our knowledge of spatial and temporal gene expression and contributes to the characterization of new promoters for research and development. The successful application of transgenic technology has been further strengthened by the availability of a broad spectrum of promoters having the ability to regulate the temporal and spatial expression patterns of the transgene. A variety of promoters is necessary at all levels of genetic engineering in plants, from basic research discoveries, to development of economically viable crops and plant commodities, to addressing legitimate concerns raised about the safety and containment of transgenic plants in the environment. Compared with the temporalor spatial-specific expression of the toxin, constitutive expression of foreign proteins in transgenic plants may cause adverse effects. Constitutive overexpression of transgenes that interfere with normal processes in a plant underscores the need for refinement of transgene expression. The development of tissue-specific promoters to drive transgene expression has helped us to fulfil that need. Therefore, in certain circumstances, it is desirable to use expression-specific promoters which express only the foreign gene in specific plant tissues or organs. This review highlights the uses and benefits reaped by researchers by using a green tissue specific promoter, RuBisCO small subunit promoter, in different crops and systems and thus establishing a broad range of tissue specific promoters. Such plant promoters that are activated precisely when and where they are needed would be ideal for genetic engineering strategies.

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Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm

Cited by 19 publications

References 28 publications

Proteomics as a tool for tapping potential of entomopathogens as microbial insecticides

Proteomics as a tool for tapping potential of entomopathogens as microbial insecticides

Genetic Improvement of Oilseed Crops Using Modern Biotechnology

Endeavours of RuBisCO small subunit promoter as a tool of green tissue specific expression

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