Expression of Bacillus thuringiensis Toxins in Insect Cells

Ribeiro, Bergmann Morais; Martins, E. S.; Aguiar, Raimundo Wagner de Souza; Corrêa, Roberto Franco Teixeira

doi:10.1007/978-3-319-56678-8_8

Cited by 2 publications

(1 citation statement)

References 55 publications

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“…Molecular mass analysis using SDS-PAGE showed the major bands of five effective isolate at approximately 130 and 65 kDa proteins, which were consistent with the 130 and 65 kDa proteins of the bipyramidal crystals of Cry1 proteins and the cuboidal crystals of Cry2 proteins, respectively (Azizoglu et al 2015;Patel et al 2012;Zorzetti et al 2017). Even Cry2 toxins which form cuboidal inclusion have been reported to exhibit dual toxicity against both lepidopteran and dipteran insects (Ribeiro et al 2017). In this work, very low toxicity against A. aegypti larvae was observed, this may be due to the five effective B. thuringiensis isolates contain Cry2 toxin which do not show larvicidal activity to A. aegypti larvae.…”

Section: Discussionsupporting

confidence: 60%

Molecular characterization of lepidopteran-specific toxin genes in Bacillus thuringiensis strains from Thailand

Boonmee

Thammasittirong

2019

3 Biotech

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A total of 511 local isolates of Bacillus thuringiensis from different geographical regions of Thailand were analyzed for the presence of the cry1A, cry1B, cry2A, cry9, and vip3A genes encoding for lepidopteran-specific toxins. PCR results revealed that 94.32% (482/511) of B. thuringiensis isolates harbored at least one of the detected genes, of which the cry1A, cry1B, cry2A, cry9, and vip3A genes were detected at frequencies of 90.61%, 89.63%, 76.32%, 40.70%, and 48.18%, respectively. Nineteen gene-combination profiles were discovered among 482 B. thuringiensis isolates, of which the most frequently detected profile contained the cry1A, cry1B, cry2A, and vip3A genes. Sixty-one isolates (12.66%), which harbored all of the detected insecticidal toxin genes, were further detected for the exochitinase (chi36) gene and chitinase activity. The results revealed that all 61 isolates contained the chi36 gene and exhibited chitinase activity. Insect bioassays showed that five isolates were highly toxic (more than 80% mortality) against second instar larvae of Spodoptera litura, of which the highest insect mortality (93%) was obtained from the B. thuringiensis isolates 225-15 and 417-1. Scanning electron microscopy revealed that the crystal morphologies of the five effective isolates were bipyramidal and cuboidal shapes. SDS-PAGE analysis of the spore-crystal mixture showed major bands of approximately 65 and 130 kDa. These five effective strains are alternative candidates for use as a microbial insecticide for the control of the S. litura pest.

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Section: Discussionsupporting

confidence: 60%

Molecular characterization of lepidopteran-specific toxin genes in Bacillus thuringiensis strains from Thailand

Boonmee

Thammasittirong

2019

3 Biotech

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Enhancing Crop Resilience in the Face of a Changing Climate

Azeem,

Ul-Allah,

Khan

et al. 2023

Water-Soil-Plant-Animal Nexus in the Era of Climate Change

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Crops can adapt to shifting environmental conditions due to the diversity of traits and characteristics that genetic diversity gives. The capacity to adapt is a fundamental component of crop resilience. Breeding and natural selection are necessary for developing hardy crop types. Breeders can create more resilient crops by subjecting them to various stresses and identifying individuals with favorable features. Agroecological approaches and sustainable farming can improve crop resilience. Crop rotation, intercropping, and agroforestry increase agroecosystem resilience and promote biodiversity. Developing crop varieties with improved resilience to extreme weather events, such as droughts, floods, and heat waves, is essential to addressing the difficulties posed by climate change. Crop resilience can be improved by utilizing agroecological methods, genetic variety, and adaptive ability. The productivity and adaptability of our agricultural systems in the face of shifting environmental conditions can be increased by creating resilient crop types.

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Expression of Bacillus thuringiensis Toxins in Insect Cells

Cited by 2 publications

References 55 publications

Molecular characterization of lepidopteran-specific toxin genes in Bacillus thuringiensis strains from Thailand

Molecular characterization of lepidopteran-specific toxin genes in Bacillus thuringiensis strains from Thailand

Enhancing Crop Resilience in the Face of a Changing Climate

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