1988
DOI: 10.1007/bf00330861
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Expression of bacterial chitinase protein in tobacco leaves using two photosynthetic gene promoters

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Cited by 58 publications
(14 citation statements)
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“…If this AUG codon occurs in a favorable context for initiation, the 40S ribosomal subunits are halted, the 60S subunits associate with them, and translation is started; if the context around the first AUG codon is less favorable, some or most of the 40S subunits proceed to the next AUG. The favorable contexts for initiation, as established by nucleotide sequence analysis and site-directed mutagenesis, are AACAAIUGGC for plant mRNAs (23,25,36,45) and (GCC)GCC'CCAUG for vertebrate mRNAs (29-31). The scanning model is in accordance with the finding that nucleus-encoded genes in eucaryotes are normally organized in monocistronic transcriptional units.…”
mentioning
confidence: 99%
“…If this AUG codon occurs in a favorable context for initiation, the 40S ribosomal subunits are halted, the 60S subunits associate with them, and translation is started; if the context around the first AUG codon is less favorable, some or most of the 40S subunits proceed to the next AUG. The favorable contexts for initiation, as established by nucleotide sequence analysis and site-directed mutagenesis, are AACAAIUGGC for plant mRNAs (23,25,36,45) and (GCC)GCC'CCAUG for vertebrate mRNAs (29-31). The scanning model is in accordance with the finding that nucleus-encoded genes in eucaryotes are normally organized in monocistronic transcriptional units.…”
mentioning
confidence: 99%
“…Construct pJJ2104 contains the bacterial chiA gene under the control of the 35S promoter from cauliflower mosaic virus, with the 3' polyadenylation sequence being derived from the nos gene (11). The chiA gene in construct 1781 is expressed from the promoter of the most highly expressed ribulose bisphosphate carboxylase small subunit (rbcS) gene from petunia, and has the polyadenylation site from the same gene ( 14). All (19) containing in addition naphthalene acetic acid and 6-benzylaminopurine (both at 0.1 Ag/mL).…”
Section: Plasmid Constructionsmentioning
confidence: 99%
“…We describe here a system which uses this approach to study protein secretion in plant cells. Our reporter protein is a bacterial chitinase (ChiA) from Serratia marcescens, which has been cloned and expressed in plant cells under a variety of promoters (11,13,14,27). We show here that a proportion of this protein is modified, probably by N-glycosylation, and transported out of plant cells in which it is expressed, showing that a bacterial signal sequence can function in plant cells.…”
mentioning
confidence: 91%
“…The ballistic device is also overcomes some of the problems associated with the use of Agrobacterium in blackgram. The Bacterial chitinase (ChiB) gene has been expressed in the leaves of tobacco using two photosynthetic gene promoters (Jonathan et al 1986) [5]. This gene has also been expressed in litchi (Das and Rahman 2010) [6].…”
Section: Methodsmentioning
confidence: 99%