Expression of Bax and Bcl-2 Proteins in Left-Ventricular Cardiomyocytes in Wistar-Kyoto and SHR Rats with Insulin-Dependent Diabetes Mellitus

Sklifasovskaya, A. P.; Благонравов, М. Л.; Ryabinina, A. Yu.; Азова, М. М.; Goryachev, V. A.

doi:10.1007/s10517-021-05272-7

Cited by 5 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This fact indicates that the activation of these proteins plays a maladaptive role in the activity of CMCs, which can serve as an important marker for assessing the progression of vascular damage in this type of pathology. A decrease in the expression of the HSP60/HSP10 complex relative to Bax was also found in all the pathological models studied, while this difference was less pronounced in the combined hypertension with DM group (43,98,143). Thus, a decrease in the production of HSP60 can be considered as one of the pathophysiological mechanisms of LV myocardial damage caused by hypertension and/or DM.…”

Section: The Role Of Hsps In Regulated Cell Deathmentioning

confidence: 75%

“…The level of Bcl-2 was also significantly increased and the Bcl-2/Bax ratio tended to increase, but remained below the control. This indicates a decrease of the apoptotic cascade in the comorbid pathology compared to isolated hypertension and DM ( 142 , 143 ).…”

Section: The Role Of Hsps In Regulated Cell Deathmentioning

confidence: 92%

“…In the mentioned study, the expression of HSP27 in LV myocardium was also evaluated. In the group with longer duration of hypertension (SHR rats aged 57 weeks), the ratio of Bax and Bcl-2 was the most pronounced, while the level of HSP27 was also the highest in all groups, which may indicate the activation of protein defense mechanisms directed to Bax binding ( 43 , 98 , 143 ).…”

Section: The Role Of Hsps In Regulated Cell Deathmentioning

confidence: 98%

See 2 more Smart Citations

The role of heat shock proteins in the pathogenesis of heart failure (Review)

Sklifasovskaya,

Blagonravov,

Ryabinina

et al. 2023

Int J Mol Med

View full text Add to dashboard Cite

The influence of heat shock proteins (HSPs) on protein quality control systems in cardiomyocytes is currently under investigation. The effect of HSPs on the regulated cell death of cardiomyocytes (CMCs) is of great importance, since they play a major role in the implementation of compensatory and adaptive mechanisms in the event of cardiac damage. HSPs mediate a number of mechanisms that activate the apoptotic cascade, playing both pro- and anti-apoptotic roles depending on their location in the cell. Another type of cell death, autophagy, can in some cases lead to cell death, while in other situations it acts as a cell survival mechanism. The present review considered the characteristics of the expression of HSPs of different molecular weights in CMCs in myocardial damage caused by heart failure, as well as their role in the realization of certain types of regulated cell death.

show abstract

Section: The Role Of Hsps In Regulated Cell Deathmentioning

confidence: 75%

Section: The Role Of Hsps In Regulated Cell Deathmentioning

confidence: 92%

Section: The Role Of Hsps In Regulated Cell Deathmentioning

confidence: 98%

See 1 more Smart Citation

The role of heat shock proteins in the pathogenesis of heart failure (Review)

Sklifasovskaya,

Blagonravov,

Ryabinina

et al. 2023

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…Interestingly, these functional alterations appear before the occurrence of morphological changes in the structure of the myocardium and microvasculature [ 112 ]. Subsequently, the activation of pro-apoptotic pathways determines the depletion of contractile myocardium which is replaced by fibrotic tissue [ 114 ], resulting in LV systo-diastolic dysfunction [ 116 ]. Moreover, chronic hyperglycemia triggers secondary pulmonary hypertension which contributes to RV hypertrophy and late systolic dysfunction (12 weeks from disease induction) [ 115 ].…”

Section: Drug Toxicity Modelsmentioning

confidence: 99%

Rodent Models of Dilated Cardiomyopathy and Heart Failure for Translational Investigations and Therapeutic Discovery

Ponzoni

Coles

Maynes

2023

IJMS

View full text Add to dashboard Cite

Even with modern therapy, patients with heart failure only have a 50% five-year survival rate. To improve the development of new therapeutic strategies, preclinical models of disease are needed to properly emulate the human condition. Determining the most appropriate model represents the first key step for reliable and translatable experimental research. Rodent models of heart failure provide a strategic compromise between human in vivo similarity and the ability to perform a larger number of experiments and explore many therapeutic candidates. We herein review the currently available rodent models of heart failure, summarizing their physiopathological basis, the timeline of the development of ventricular failure, and their specific clinical features. In order to facilitate the future planning of investigations in the field of heart failure, a detailed overview of the advantages and possible drawbacks of each model is provided.

show abstract

Alpha-lipoic acid enhances ischemic postconditioning-mediated improvement of myocardial infarction and apoptosis in diabetic rats with ischemia/reperfusion injury

Gholami,

Badalzadeh,

Alihemmati

2023

Can. J. Physiol. Pharmacol.

View full text Add to dashboard Cite

This work evaluated the combined effects of alpha-lipoic acid (ALA) and ischemic-postconditioning (Post) on myocardial infarction and cell death in rats with chronic type-2 diabetes following ischemia/reperfusion injury. The rats received a high-fat diet and were given one intraperitoneal injection of 35 mg/kg streptozotocin to induce chronic diabetes. They were then pretreated with ALA (100 mg/kg/day, orally) for five weeks before undergoing I/R insult. The hearts experienced 35-minute regional ischemia through ligating the left anterior descending coronary artery, followed by 60-minute reperfusion. The Post protocol involved six cycles of a 10/10 seconds algorithm, applied during the early stage of reperfusion. The use of Post alone did not significantly alter LDH and infarct size (IS) levels, while ALA showed positive effects. Similar findings were observed for apoptotic changes with single treatments. However, the concurrent administration of ALA and Post significantly reduced the protein expression of Bax, Bax/Bcl2, and cleaved caspase-3, while increasing Bcl2 expression. Additionally, the histopathological findings of the combined therapy were superior to those of single treatments. The concomitant use of ALA and Post effectively inhibited apoptosis, leading to cardiac recovery after I/R injury in diabetic conditions. This strategy could improve outcomes for preserving diabetic hearts following I/R insults.

show abstract

Expression of Bax and Bcl-2 Proteins in Left-Ventricular Cardiomyocytes in Wistar-Kyoto and SHR Rats with Insulin-Dependent Diabetes Mellitus

Cited by 5 publications

References 14 publications

The role of heat shock proteins in the pathogenesis of heart failure (Review)

The role of heat shock proteins in the pathogenesis of heart failure (Review)

Rodent Models of Dilated Cardiomyopathy and Heart Failure for Translational Investigations and Therapeutic Discovery

Alpha-lipoic acid enhances ischemic postconditioning-mediated improvement of myocardial infarction and apoptosis in diabetic rats with ischemia/reperfusion injury

Contact Info

Product

Resources

About