Expression of Bcl-2 and Bax proteins in relation to quality of bovine oocytes and embryos produced in vitro

Yang, Ming; Rajamahendran, R.

doi:10.1016/s0378-4320(01)00186-5

Cited by 225 publications

(180 citation statements)

References 28 publications

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“…Data from the current study indicate that BAX expression was greater (P \ 0.05) in embryos produced from undefined media-containing serum than those produced in its absence, suggesting that over expression of the BAX gene, in the cellular apoptotic pathway takes place (Yang and Rajamahendran 2002;Opiela et al 2008). This non-regulation of BAX mRNA expression could cause low quality of embryos in undefined media.…”

Section: Discussionmentioning

confidence: 60%

“…Another significant finding from this study was that the level of BCL-2 mRNA in defined medium increased (P \ 0.05) at 2-cell, 8-to 16-cell, morula and hatched blastocyst stages than for embryos from undefined media. Yang and Rajamahendran (2002) demonstrated that good quality embryos have a greater concentration of BCL-2 protein than BAX protein, whereas there is more BAX than BCL-2 in lower quality embryos. Correspondingly, in this study the apoptotic incidence of embryos derived from defined medium at 2-cell, morula and hatched blastocyst stages were significantly lower than undefined media embryos.…”

Section: Discussionmentioning

confidence: 99%

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Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented with 10 % FBS and defined PVA-myo-inositol-phosphate-EGF medium. No (P [ 0.05) differences at 2-cell, 4-cell and 8-cell to 16-cell stages were observed among the three cultured media used, however, increased (P \ 0.05) blastocyst yield, cell number and hatching rate were found in defined medium compared to undefined media. The expression patterns of genes implicated in embryo metabolism (GLUT-1), anti-apoptosis (BCL-2), imprinting (IGF-2R), DNA methylation (DNMT-3A) and maternal recognition of pregnancy (IFNT) were increased (P \ 0.05) in hatched blastocysts derived from defined medium compared to undefined media. In conclusion, serum-free, defined medium improved developmental competence of in vitro cultured buffalo embryos. Whether these differences in morphological development and gene expression have long-term effects on buffalo calves born after embryo transfer remains unknown. However, it is possible that early adaptations of the preimplantation embryo to its environment persist during fetal and post-natal development.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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“…However, this condition can be alleviated by using vitrification, but it must be considered that different factors in vitrification such as high cryoprotectant concentrations, cooling, and osmotic stress may contribute to the initiation of apoptosis that threaten later viability and development [16]. However, in the absence of cryopreservation, the process of in vitro maturation also induces degeneration via an apoptotic pathway [13][14][15]. This pathway is also activated by such physiological stimuli as: temperature, toxicants, and oxidative stress, to name a few [16].…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Ebrahimi

Valojerdi

Eftekhari‐Yazdi

et al. 2010

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities. Methods Freshly isolated (control group) and vitrifiedwarmed COCs (cryotop group) were matured in vitro. The expression of pro-and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. Results The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25±3.01% and 51.94±2.7% respectively. The expression rates of proand anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p<0.05). Conclusion Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.

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“…The data from the current study indicate that, BAX expression showed a steady increase through the developmental stages and the levels were significantly (P \ 0.05) higher in NT embryos from AFs than in embryos from AFSCs and IVF, suggesting that overexpression of the BAX gene accelerates cellular apoptotic pathways (Yang and Rajamahendran 2002;Opiela et al 2008). This non-regulation of BAX mRNA production could be a cause for the low quality of the cloned embryos.…”

Section: Discussionmentioning

confidence: 65%

“…Transcript abundance of cell death regulatory genes may be altered in embryos undergoing fragmentation, so that expression of the gene involved in cell death (BAX) is elevated, and expression of the gene involved in cell survival (BCL2) may be reduced. Yang and Rajamahendran (2002) demonstrated that good quality embryos have a greater concentration of BCL2 protein than that of BAX protein, while there is more BAX than BCL2 in lower quality embryos. The higher apoptotic incidence of SCNT embryos than those of the IVF group may be attributed to the in vitro micromanipulation (McElroy et al 2008).…”

Section: Discussionmentioning

confidence: 99%

A comparative study on expression profile of developmentally important genes during pre-implantation stages in buffalo hand-made cloned embryos derived from adult fibroblasts and amniotic fluid derived stem cells

Shah

Kataria

et al. 2015

Cytotechnology

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Abnormal gene expression in somatic cell nuclear transfer embryos due to aberrant epigenetic modifications of the donor nucleus may account for much of the observed diminished viability and developmental abnormalities. The present study compared the developmentally important gene expression pattern at 4-cell, 8-to 16-cell, morula, and blastocyst stages of buffalo nuclear transfer (NT) embryos from adult fibroblasts (AFs) and amniotic fluid stem cells (AFSCs). In vitro fertilized embryos were used as control embryos. Alterations in the expression pattern of genes implicated in transcription and pluripotency (OCT4, STAT3, NANOG), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), growth factor signaling, and imprinting (IGF2, IGF2R), apoptosis (BAX, BCL2), oxidative stress (MnSOD), metabolism (GLUT1) regulation were observed in cloned embryos. The expression of transcripts in AFSC-NT embryos more closely followed that of the in vitro fertilized embryos compared with AF-NT embryos. It is concluded that AFSCs with a relatively undifferentiated genome may serve as suitable donors which could be reprogrammed more efficiently to reactivate expression of early embryonic genes in buffalo NT.

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Expression of Bcl-2 and Bax proteins in relation to quality of bovine oocytes and embryos produced in vitro

Cited by 225 publications

References 28 publications

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

A comparative study on expression profile of developmentally important genes during pre-implantation stages in buffalo hand-made cloned embryos derived from adult fibroblasts and amniotic fluid derived stem cells

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