Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants

Biswas, G. C. Ghosh; Ransom, Callista; Sticklen, Mariam B.

doi:10.1016/j.plantsci.2006.06.004

Cited by 93 publications

(63 citation statements)

References 32 publications

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“…Another identified protein whose expression decreased in triticale roots under moderate osmotic stress was endoglucanase 10, which is capable of degradation of cell wall components and thus involved in cell elongation (Biswas et al 2006;Geilfus et al 2011). Studies performed on maize showed that the decrease of cellulases in the leaves may be associated with a lower tolerance to salt stress and inhibition of plant growth (Geilfus et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

2013

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Osmotic stress causes many adverse symptoms in plants, which include, for example, growth limitation and decrease or even absence of yield. Proteomic analyses of plant responses to stressors could lead to the introduction of crops with high resistance to osmotic stress. Such plants would be characterized by high yield even under unfavorable environmental conditions. In this article we describe changes in the protein profiles occurring in response to mild and moderate osmotic stress in triticale roots. Analysis of the protein profiles of these roots showed an increased abundance of 14 and a decreased abundance of 11 proteins under mild osmotic stress conditions while a moderate osmotic stress caused an increased abundance of 18 and a decreased abundance of 33 proteins. Twenty-five proteins, whose quantity altered under stress were identified using MALDI-TOF mass spectrometry. The identified proteins were classified into the categories of proteins associated with: defense mechanisms, metabolism, transcription, cell structure, protein synthesis, transport and signal transduction. The functions of identified proteins were discussed in relation to osmotic stress. Some of the identified proteins may be responsible for the adaptation of plants to adverse conditions.

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Section: Discussionmentioning

confidence: 99%

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

2013

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“…Thermophilic cell wall-degrading enzymes with optimal temperatures over 50°C can be expressed in plants without disrupting cell wall integrity because they tend to perform poorly in normal plant growth conditions [76]. Hydrolases from thermophilic microorganisms such as Acidothermus cellulolyticus and Thermomonospora fusca, which are able to hydrolyze cellulose at high temperatures (81 and 75°C respectively), have been expressed in various plant species with no harmful effects [37,56,[77][78][79], while still having the desired effect of improving the ease of breakdown of plant material after harvest. Moreover, thermophilic enzymes are activated during post-harvest processing providing more advantages and applications in cellulosic ethanol production including simplifying processing and reducing exogenous enzyme loading [38].…”

Section: Strategies To Prevent Harmful Effects Of Glycosyl Hydrolase mentioning

confidence: 99%

“…Feedstocks with established genetic modification technology such as maize, rice, sugarcane, and poplar have been successfully transformed to express cell wall-degrading enzymes [55,77,79,80]. Several examples of expression of cell walldegrading enzymes in lignocellulosic biomass feedstock are provided in Table 1.…”

Section: Expression Of Glycosyl Hydrolases In Lignocellulosic Feedstockmentioning

confidence: 99%

Expression of Glycosyl Hydrolases in Lignocellulosic Feedstock: An Alternative for Affordable Cellulosic Ethanol Production

2016

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Ethanol produced from lignocellulosic feedstock is a promising alternative to fossil fuels and corn-sourced ethanol. However, it creates unique challenges in terms of requirements for breakdown to fermentable sugars, including the need for pretreatment and enzymatic hydrolysis of structural carbohydrates. Hydrolases from microorganisms are currently utilized for biomass hydrolysis in the production of lignocellulosic ethanol; however, expressing these hydrolases in lignocellulosic feedstock is a favorable alternative, due to the large availability of biomass and the potential for the feedstock to play a dual role as both biomass substrate and enzyme provider. This review summarizes recent achievements in hydrolytic enzyme expression in a variety of model plants and potential feedstocks, including strategies to improve enzyme yield and to prevent deleterious effects on plants hosts. We propose possible scenarios for utilizing enzyme-expressing transgenic feedstock and illustrate the potential benefits of using these crops for ethanol production. Furthermore, challenges are highlighted and potential solutions proposed to move the field forward to cost-comparable lignocellulosic ethanol.

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“…These new promising strategies include (1) increasing cellulose composition (Coleman et al, 2009), (2) reducing plant cell wall recalcitrance and cellulose crystallinity (Fry et al, 2008), (3) producing cellulases and other protein modules which are necessary for disruption of plant cell wall substrates (Vaaje-Kolstad et al, 2005;Biswas et al, 2006;Cosgrove et al, 2007;Mei et al, 2009;Park et al, 2011), and (4) reducing lignin content in plants by down-regulation of lignin biosynthesis (Ralph et al, 2006) or diverting lignin biosynthesis towards cellulose synthesis (Chen and Dixon, 2007;Haghighi Mood et al, 2013).…”

Section: New Pretreatment Technologiesmentioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants

Cited by 93 publications

References 32 publications

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

Expression of Glycosyl Hydrolases in Lignocellulosic Feedstock: An Alternative for Affordable Cellulosic Ethanol Production

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

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