Expression of biotic stress response genes to Phytophthora infestans inoculation in White Lady, a potato cultivar with race-specific resistance to late blight

Hajianfar, Ramin; Kolics, Balázs; Cernák, István; Wolf, I.; Polgár, Zsolt; Taller, János

doi:10.1016/j.pmpp.2015.12.001

Cited by 9 publications

(4 citation statements)

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“…As it has been mentioned before two conventionally bred varieties (Toluca and Bionica) possess the Rpi-blb2 gene introgressed from S. bulbocastanum to common potato for a 46year period (Haverkort et al, 2009). Additionally, the Rpi-blb2 homolog has been detected in Hungarian cultivar White Lady (Hajianfar et al, 2016). The present paper represents a first report of finding the RB/Rpi-blb1 = Rpi-sto1-like sequences in conventional bred varieties.…”

Section: Discussionsupporting

confidence: 64%

Finding RB/Rpi-blb1/Rpi-sto1-like sequences in conventionally bred potato varieties

et al. 2018

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The main objectives in potato breeding are increasing yield abilities and improving resistance to numerous pathogens and pests. Among them, the late blight caused by the Phytophthora infestans oomycete is one of the most destructive potato diseases both in Russia and worldwide. Wild relatives of cultivated potato are traditionally used in breeding as the source of valuable R genes conferring resistance to pathogens. Of particular interest are Mexican wild species because Mexico is the centre of origin and diversity of P. infestans and at the same time, it is the centre of potato species diversity. Mexican wild potato species S. bulbocastanum and S. stoloniferum are an important source of the R genes conferring broad-spec trum resistance against various isolates of P. infestans (Rpi-blb1, Rpi-blb2, Rpi-sto1). Recently these genes have been transferred into cultivated potato gene pool using the cisgene approach. At the same time there is a high probability of finding geno types with the Rpi-sto1 gene (functional homologues of Rpi-blb1) among conventionally bred varieties because for about 40 years S. stoloniferum has been used in breeding as a source of the Rysto and Ry-fsto genes of the extreme resistance to the most important viral pathogen PVY. In this study 188 potato varieties bred in Russia and in near-abroad countries were screened for the presence of six gene-specific markers of the RB/Rpi-blb1 = Rpi-sto1 and Rpi-blb2 genes conferring broad-spectrum resistance against P. infestans, and for the markers linked to the Rysto and Ry-fsto genes conferring extreme resistance to PVY. In addition, a marker for detecting male sterile mitochondrial DNA type gamma derived from S. stoloniferum was used. The genotypes selected through the molecular markers were divided into four groups: (A) 13 PVY resistant varieties carrying diagnostic markers of the Rysto, Ry-fsto genes and having sterile mt-type gamma; (B) four varieties possessing mt-type gamma and not having the markers of the R genes introgressed from S. stoloniferum; (C) eight genotypes carrying five gene-specific markers for the RB/Rpi-blb1/= Rpi-sto1; (D) the rest 166 (86.9 %) varieties not possessing any of the diagnostic markers associated with the S. stoloniferum genetic material. The sequences of the Rpi-sto1- and BLB1 F/R-amplicons were identical in all the genotypes of group ‘C’ and showed respective 99 % and 100 % similarity to the corresponding fragments of the Rpi-sto1 and Rpi-blb1 genes from the GenBank database. Among the genotypes of group ‘C’ various mt-types were detected, and some of them were male fertile.

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Section: Discussionsupporting

confidence: 64%

Finding RB/Rpi-blb1/Rpi-sto1-like sequences in conventionally bred potato varieties

et al. 2018

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“…and also for some viruses [ 12 , 13 ]. Late blight, is one of the most serious potato diseases worldwide [ 14 ]. However, it was shown that bittersweet has a minimal role in late blight infections since most plants are resistant and the inocula of the pathogen do not overwinter [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

The chloroplast genome sequence of bittersweet (Solanum dulcamara): Plastid genome structure evolution in Solanaceae

2018

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Bittersweet (Solanum dulcamara) is a native Old World member of the nightshade family. This European diploid species can be found from marshlands to high mountainous regions and it is a common weed that serves as an alternative host and source of resistance genes against plant pathogens such as late blight (Phytophthora infestans). We sequenced the complete chloroplast genome of bittersweet, which is 155,580 bp in length and it is characterized by a typical quadripartite structure composed of a large (85,901 bp) and small (18,449 bp) single-copy region interspersed by two identical inverted repeats (25,615 bp). It consists of 112 unique genes from which 81 are protein-coding, 27 tRNA and four rRNA genes. All bittersweet plastid genes including non-functional ones and even intergenic spacer regions are transcribed in primary plastid transcripts covering 95.22% of the genome. These are later substantially edited in a post-transcriptional phase to activate gene functions. By comparing the bittersweet plastid genome with all available Solanaceae sequences we found that gene content and synteny are highly conserved across the family. During genome comparison we have identified several annotation errors, which we have corrected in a manual curation process then we have identified the major plastid genome structural changes in Solanaceae. Interpreted in a phylogenetic context they seem to provide additional support for larger clades. The plastid genome sequence of bittersweet could help to benchmark Solanaceae plastid genome annotations and could be used as a reference for further studies. Such reliable annotations are important for gene diversity calculations, synteny map constructions and assigning partitions for phylogenetic analysis with de novo sequenced plastomes of Solanaceae.

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“…Doke 1985; Király et al 2008;Dubiella et al 2013;Kadota et al 2015) are supported by studies showing that absence of expression of NADPH oxidase genes may confer enhanced susceptibility to hemibiotrophic pathogens, e.g. Phytophthora infestans (Yoshioka et al 2003;Hajianfar et al 2016) and biotrophic pathogens (i.e. fully preferring live host tissues) like powdery mildews.…”

Section: Discussionmentioning

confidence: 99%

Graft-transmissible resistance of cherry pepper (Capsicum annuum var. cerasiforme) to powdery mildew (Leveillula taurica) is associated with elevated superoxide accumulation, NADPH oxidase activity and pathogenesis-related gene expression

et al. 2017

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We found that resistance to pepper powdery mildew (PM) (Leveillula taurica) develops in a sweet pepper (Capsicum annuum) cultivar ('Totál') when grafted on a resistant cherry pepper (C. annuum var. cerasiforme) rootstock (cv. Szentesi). Resistance is manifested both towards PM symptoms and pathogen accumulation. In healthy, uninfected plants PM-resistance can be predicted by enhanced accumulation of the reactive oxygen species (ROS) superoxide (O 2 .2) and activity of NADPH oxidase, the enzyme mainly responsible for pathogenesis-related superoxide generation. In L. taurica-inoculated PM-resistant 'Szentesi' high levels of superoxide and NADPH oxidase activity are sustained even 45 days after inoculation, as opposed to PM-susceptible 'Totál'. This is also true for 'Totál' grafted on resistant 'Szentesi' rootstocks, where PM resistance, enhanced superoxide production and NADPH oxidase activity is likely due to an unknown, graft-transmitted signal. To further elucidate the mechanisms of graft-transmissible PM-resistance we monitored expression of pathogenesis-related (PR) genes in healthy and infected plants. In healthy plants, expression of CaPR-1 is several times higher in leaves of PM-resistant pepper than in sensitive plants, while high expression of CaPR-2 (glucanase) does not entirely correlate with PM-resistance, being detectable only in PM-resistant 'Szentesi'. However, during advanced stages of PM-pathogenesis (45 DAI) expression of CaPR-1 and CaPR-2 is by far the highest in PM-susceptible 'Totál'. Our results suggest that the direct biochemical cause of graft-transmissible PM-resistance in pepper is the enhanced accumulation of NADPH oxidasegenerated superoxide. To our knowledge, this is the first report on the role of ROS (superoxide) in graft-transmissible, pathogen-specific disease resistance. Keywords Capsicum annuum var. cerasiforme Á Grafttransmissible resistance Á Leveillula taurica Á Superoxide Á NADPH oxidase Á Pathogenesis-related genes Communicated by E Kuzniak-Gebarowska.

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Expression of biotic stress response genes to Phytophthora infestans inoculation in White Lady, a potato cultivar with race-specific resistance to late blight

Cited by 9 publications

References 42 publications

Finding RB/Rpi-blb1/Rpi-sto1-like sequences in conventionally bred potato varieties

Finding RB/Rpi-blb1/Rpi-sto1-like sequences in conventionally bred potato varieties

The chloroplast genome sequence of bittersweet (Solanum dulcamara): Plastid genome structure evolution in Solanaceae

Graft-transmissible resistance of cherry pepper (Capsicum annuum var. cerasiforme) to powdery mildew (Leveillula taurica) is associated with elevated superoxide accumulation, NADPH oxidase activity and pathogenesis-related gene expression

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