Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods

Abstract

Objective: Yersinia enterocolitica which is an important foodborne pathogen causing illness in humans is an extremely heterogeneous species consisting of different subtypes. It has the intrinsic resistance to β-lactam antibiotics because of the production of β-lactamases, BlaA and BlaB. Y. enterocolitica exhibits variable susceptibilities to β-lactams. Materials and Methods:The expression of the blaA and blaB genes by polymerase chain reaction, and the susceptibility to some β-lactams including penicillins and cephalosporins using the broth microdilution and disk diffusion methods were determined. A total of 18 Y. enterocolitica isolates were examined.Results: Overall, 33.3% of these isolates carried the blaA and blaB genes, all of which were recovered from chicken meat. The wide range of MIC for ampicillin (≤2-128 µg/mL) and ceftazidime (≤0.0625-2 µg/mL) was also observed. Of the Y. enterocolitica isolates, 55.6% were resistant to ampicillin (≥32 µg/mL) while the remaining isolates (44.4%) were susceptible to ampicillin (≤ 8 µg/mL). All isolates were susceptible to ceftazidime at the concentration tested. According to the disk diffusion test, 55.6% and 33.3% of the isolates were resistant to ticarcillin and cefoxitin, respectively. No resistance to piperacillin and ceftriaxone was found. Conclusion:The results showed that the presence of the blaA and blaB genes and intrinsic resistance against penicillins and cephalosporins were variable among Y. enterocolitica food isolates. Furthermore, the blaA and blaB genes were expressed in most of the resistant isolates to β-lactams, which may indicate the contribution of the genes to the drug resistance.

Detection, characterization, and antimicrobial susceptibility of Yersinia enterocolitica in different types of wastewater in the Czech Republic

“…EUCAST has recognized Y. enterocolitica as intrinsically resistant to ampicillin, amoxicillin, amoxicillin-clavulanic acid, ampicillin-sulbactam, ticarcillin, cefazolin, cephalothin, cefalexin, cefadroxil, and cefoxitin (EUCAST, 2022). However, due to the considerable variability in the expression and activity of these two βlactamases as well as the extreme heterogeneity of Y. enterocolitica, differences in the level and spectrum of resistance to these antibiotics are observed between strains (Fàbrega & Vila, 2012;Özdemir et al, 2020). This phenomenon was also found in our study.…”

“…However, due to the considerable variability in the expression and activity of these two β‐lactamases as well as the extreme heterogeneity of Y . enterocolitica , differences in the level and spectrum of resistance to these antibiotics are observed between strains (Fàbrega & Vila, 2012; Özdemir et al, 2020). This phenomenon was also found in our study.…”

“…In contrast, resistance to βlactam antibiotics in Y. enterocolitica is well described. The most common mechanism of resistance is mediated by the chromosomal genes blaA and blaB, which encode constitutively produced βlactamases (Fàbrega & Vila, 2012;Özdemir et al, 2020). EUCAST has recognized Y. enterocolitica as intrinsically resistant to ampicillin, amoxicillin, amoxicillin-clavulanic acid, ampicillin-sulbactam, ticarcillin, cefazolin, cephalothin, cefalexin, cefadroxil, and cefoxitin (EUCAST, 2022).…”

“…Most antibiotics are active against Y . enterocolitica , except for selected penicillins and cephalosporins due to natural resistance (Fois et al, 2018; Özdemir et al, 2020; Ye et al, 2015).…”

Clonalidade e perfis de resistência a antibióticos em Yersinia enterocolitica de suínos e humanos no Brasil

“…The expression of this gene varies among Yersinia strains, depending mainly on the subtype and geographical origin of the isolates. Y. enterocolitica isolates obtained from swine and poultry usually present resistance to ampicillin; similarly, isolates from humans also present a high rate of resistance to ampicillin (ÖZDEMIR et al, 2020). All isolates harbored y56-beta-lactamase, a heterogeneous blaA gene among Y. enterocolitica from biotype 1A, and presenting low homology to other Enterobacteriaceae β-lactamase-related genes (SHARMA et al, 2006).…”