Expression of Bordetella pertussis fimbrial (fim) genes in Bordetella bronchiseptica: fimX is expressed at a low level and vir-regulated

Riboli, B.; Pedroni, Paola Maria; Cuzzoni, A.; Grandi, Guido; Ferra, Francesca de

doi:10.1016/0882-4010(91)90084-n

Cited by 19 publications

(17 citation statements)

References 21 publications

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“…The fimX gene is homologous to fim2 and fim3, but its product has not been identified yet, and the expression of this gene has so far only been detected in B. bronchiseptica, where it has been identified as a vag (Riboli et al 1991).…”

Section: Discussionmentioning

confidence: 95%

Differential modulation of Bordetella pertussis virulence genes as evidenced by DNA microarray analysis

et al. 2003

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The production of most factors involved in Bordetella pertussis virulence is controlled by a two-component regulatory system termed BvgA/S. In the Bvg+ phase virulence-activated genes (vags) are expressed, and virulence-repressed genes (vrgs) are down-regulated. The expression of these genes can also be modulated by MgSO(4) or nicotinic acid. In this study we used microarrays to analyse the influence of BvgA/S or modulation on the expression of nearly 200 selected genes. With the exception of one vrg, all previously known vags and vrgs were correctly assigned as such, and the microarray analyses identified several new vags and vrgs, including genes coding for putative autotransporters, two-component systems, extracellular sigma factors, the adenylate cyclase accessory genes cyaBDE, and two genes coding for components of a type III secretion system. For most of the new vrgs and vags the results of the microarray analyses were confirmed by RT-PCR analysis and/or lacZfusions. The degree of regulation and modulation varied between genes, and showed a continuum from strongly BvgA/S-activated genes to strongly BvgA/S-repressed genes. The microarray analyses also led to the identification of a subset of vags and vrgs that are differentially regulated and modulated by MgSO(4) or nicotinic acid, indicating that these genes may be targets for multiple regulatory circuits. For example, the expression of bilA, a gene predicted to encode an intimin-like protein, was found to be activated by BvgA/S and up-modulated by nicotinic acid. Furthermore, surprisingly, in the strain analysed here, which produces only type 2 fimbriae, the fim3 gene was identified as a vrg, while fim2 was confirmed to be a vag.

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Section: Discussionmentioning

confidence: 95%

Differential modulation of Bordetella pertussis virulence genes as evidenced by DNA microarray analysis

et al. 2003

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“…The major fimbrial subunits that form the two predominant Bordetella fimbrial serotypes, Fim2 and Fim3 (AGG2 and AGG3), are encoded by unlinked chromosomal loci fim2 and fim3, respectively (470,558). A third unlinked locus, fimX, is expressed only at very low levels if at all (660), and recently a fourth fimbrial locus, fimN, was identified in B. bronchiseptica (401 (638).…”

Section: Virulence Determinantsmentioning

confidence: 99%

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

2005

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Bordetella respiratory infections are common in people (B. pertussis) and in animals (B. bronchiseptica). During the last two decades, much has been learned about the virulence determinants, pathogenesis, and immunity of Bordetella. Clinically, the full spectrum of disease due to B. pertussis infection is now understood, and infections in adolescents and adults are recognized as the reservoir for cyclic outbreaks of disease. DTaP vaccines, which are less reactogenic than DTP vaccines, are now in general use in many developed countries, and it is expected that the expansion of their use to adolescents and adults will have a significant impact on reducing pertussis and perhaps decrease the circulation of B. pertussis. Future studies should seek to determine the cause of the unique cough which is associated with Bordetella respiratory infections. It is also hoped that data gathered from molecular Bordetella research will lead to a new generation of DTaP vaccines which provide greater efficacy than is provided by today's vaccines

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“…The molecular mechanism of this phenotypic switch is expansion and contraction of an HPT in bvgS [24], which encodes a key regulator of virulence gene expression (reviewed in [25]). In addition, phase-variable expression of two fimbrial major subunit genes, fim2 and fim3 , has been attributed to variations in HPTs located within the promoter regions of these genes that lead to altered transcript abundance [26,27]. In this study, we sought to identify additional B. pertussis candidate phase-variable genes, particularly those with potential roles in pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

Phase variation and microevolution at homopolymeric tracts in Bordetella pertussis

et al. 2007

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Background: Bordetella pertussis, the causative agent of whooping cough, is a highly clonal pathogen of the respiratory tract. Its lack of genetic diversity, relative to many bacterial pathogens, could limit its ability to adapt to a hostile and changing host environment. This limitation might be overcome by phase variation, as observed for other mucosal pathogens. One of the most common mechanisms of phase variation is reversible expansion or contraction of homopolymeric tracts (HPTs).

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Expression of Bordetella pertussis fimbrial (fim) genes in Bordetella bronchiseptica: fimX is expressed at a low level and vir-regulated

Cited by 19 publications

References 21 publications

Differential modulation of Bordetella pertussis virulence genes as evidenced by DNA microarray analysis

Differential modulation of Bordetella pertussis virulence genes as evidenced by DNA microarray analysis

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

Phase variation and microevolution at homopolymeric tracts in Bordetella pertussis

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