Expression of brain specific chondroitin sulfate proteoglycans, neurocan and phosphacan, in the developing and adult hippocampus of Ihara’s epileptic rats

Kurazono, Sekiko; Okamoto, Masahiro; Sakiyama, Junko; Mori, Shuji; Nakata, Yasunari; Fukuoka, Junya; Amano, Shigeru; Oohira, Atsuhiko; Matsui, Hideki

doi:10.1016/s0006-8993(01)02128-x

Cited by 34 publications

(21 citation statements)

References 67 publications

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“…This is significant because high levels of CSPG in SR could act as an inhibitory boundary (arrow) that prevents MFs from migrating outside of the SL. This pattern is also very similar to that previously reported using the IG2 antibodies to identify neurocan (Kurazono et al, 2001). Extracellular phosphacan immunostaining was performed using the 3F8 (Fig.…”

Section: Distribution Of Proteoglycans In Hippocampussupporting

confidence: 91%

“…This inhibitory CSPG boundary surrounding the MFs in SL was also observed by Kurazono et al (2001), who suggested that CSPGs play an important role in preventing MFs from sprouting outside of SL after seizure activity. However, presently there is no direct functional evidence for the involvement of CSPGs in directing MF outgrowth during either early or late MF development.…”

Section: Introductionsupporting

confidence: 72%

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Keratan Sulfate Proteoglycan Phosphacan Regulates Mossy Fiber Outgrowth and Regeneration

Butler

Schnetz

et al. 2004

J. Neurosci.

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We have examined the role of chondroitin sulfate proteoglycans (CSPGs) and keratan sulfate proteoglycans (KSPGs) in directing mossy fiber (MF) outgrowth and regeneration in rat hippocampal slice cultures. MFs normally exhibit a very specific innervation pattern that is restricted to the stratum lucidum (SL). In addition, MFs in hippocampal slice cultures will regenerate this specific innervation pattern after transection. CSPGs are one of the best characterized inhibitory axon guidance molecules in the CNS and are widely expressed in all areas of the hippocampus except SL. KSPGs are also widely expressed in the hippocampus, but their role in axon outgrowth has not been extensively studied in the CNS where phosphacan is the only protein that appears to contain KS-GAGs. Cultured hippocampal slices were treated with either chondroitin ABC lyase or keratanases to reduce the inhibitory axon guidance properties of CS and KS proteoglycans, respectively. The ability of transected MFs to regenerate their normal innervation pattern after digestion of CS and KS-GAGS sugars with these enzymes was examined. Only keratanase treatment resulted in misrouting of MFs. Identifying the mechanism by which keratanase produced MF misrouting is complicated by the presence of splice variants of the phosphacan gene that include the extracellular form of phosphacan and the transmembrane receptor protein tyrosine phosphatase ␤/ (RPTP␤/). Both forms of phosphacan are made by astrocytes, suggesting that keratanase alters MF outgrowth by modifying astrocyte function.

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Section: Distribution Of Proteoglycans In Hippocampussupporting

confidence: 91%

Section: Introductionsupporting

confidence: 72%

Keratan Sulfate Proteoglycan Phosphacan Regulates Mossy Fiber Outgrowth and Regeneration

Butler

Schnetz

et al. 2004

J. Neurosci.

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“…Immunostaining for neurocan reveals a sharp border of expression with high levels in CA3 stratum radiatum and low levels within stratum lucidum (Kurazono et al, 2001;Butler et al, 2004). Neurocan stimulation of retinal ganglion cells disassociates N-cadherin from actin, presumably by binding a GPI-linked cell surface glycosyltransferase that can form a complex with N-cadherin (Li et al, 2000).…”

Section: Role Of Cadherin-8 In Mossy Fiber Targetingmentioning

confidence: 99%

Cadherin‐8 and N‐cadherin differentially regulate pre‐ and postsynaptic development of the hippocampal mossy fiber pathway

et al. 2007

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Cells sort into regions and groups in part by their selective surface expression of particular classic cadherins during development. In the nervous system, cadherin-based sorting can define axon tracts, restrict axonal and dendritic arbors to particular regions or layers, and may encode certain aspects of synapse specificity. The underlying model has been that afferents and their targets hold in common the expression of a particular cadherin, thereby providing a recognition code of homophilic cadherin binding. However, most neurons express multiple cadherins, and it is not clear whether multiple cadherins all act similarly in shaping neural circuitry. Here we asked how two such cadherins, cadherin-8 and N-cadherin, influence the guidance and differentiation of hippocampal mossy fibers. Using organotypic hippocampal cultures, we find that cadherin-8 regulates mossy fiber fasciculation and targeting, but has little effect on CA3 dendrites. In contrast, N-cadherin regulates mossy fiber fasciculation, but has little impact on axonal growth and targeting. However, N-cadherin is essential for CA3 dendrite arborization. Both cadherins are required for formation of proper numbers of presynaptic terminals. Mechanistically, such differential actions of these two cadherins could, in theory, reflect coupling to distinct intracellular binding partners. However, we find that both cadherins bind b-catenin in dentate gyrus (DG). This suggests that cadherins may engage different intracellular signaling cascades downstream of b-catenin, coopt different extracellular binding partners, or target distinct subcellular domains. Together our findings demonstrate that cadherin-8 and N-cadherin are critical for generating the mossy fiber pathway, but that each contributes differentially to afferent and target differentiation, thereby complementing one another in the assembly of a synaptic circuit.

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“…Certain neuron populations are ensheathed by perineuronal nets, which consist of chondroitin sulfate proteoglycans, complexed with hyaluronan and colocalized with tenascins (20 -22). The perineuronal nets develop postnatally and contribute to cell migration, neurite outgrowth, synaptogenesis, and maintenance of synaptic structure and function in the developing and adult brain (21,23).…”

Section: Grading Of Prc Signal Intensity On Turbo Flair Mr Images Accmentioning

confidence: 99%

Perirolandic Cortex of the Normal Brain: Low Signal Intensity on Turbo FLAIR MR Images

Karaarslan¹,

Arslan²

2003

Radiology

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Expression of brain specific chondroitin sulfate proteoglycans, neurocan and phosphacan, in the developing and adult hippocampus of Ihara’s epileptic rats

Cited by 34 publications

References 67 publications

Keratan Sulfate Proteoglycan Phosphacan Regulates Mossy Fiber Outgrowth and Regeneration

Keratan Sulfate Proteoglycan Phosphacan Regulates Mossy Fiber Outgrowth and Regeneration

Cadherin‐8 and N‐cadherin differentially regulate pre‐ and postsynaptic development of the hippocampal mossy fiber pathway

Perirolandic Cortex of the Normal Brain: Low Signal Intensity on Turbo FLAIR MR Images

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