Expression of c-type lysozyme gene in sea cucumber (Apostichopus japonicus) is highly regulated and time dependent after salt stress

Tian, Yi; Liang, Xue-wang; Chang, Yaqing; Jiang, Song

doi:10.1016/j.cbpb.2014.10.004

Cited by 19 publications

(9 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The latest studies put forward that adult sea cucumbers can tolerate a broader salinity ranging from 20 to 39 psu (Yuan et al 2006, 2010; Bai et al 2015). At present, the optimal salinity for A. japonicus growth is reported 29–32 psu (Tian et al 2015). Some results indicated that sea cucumbers exhibit hypo-osmotic regulation in low salinity media and show hyper-osmotic regulation in high salinity media (Binyon 1972; Diehl 1986).…”

Section: Introductionmentioning

confidence: 99%

Effect of acute salinity stress on ion homeostasis, Na+/K+-ATPase and histological structure in sea cucumber Apostichopus japonicus

et al. 2016

Self Cite

View full text Add to dashboard Cite

BackgroundSea cucumbers (Apostichopus japonicus) are an imperiled fauna exposed to a variety of environmental condition such as salinity and studies are urgently needed to assess their effects to guide aquaculture efforts. The effects of acute salinity stress on coelomic fluid osmotic pressure, ion concentrations, the activity of Na+/K+-ATPase in respiratory trees and the histological variations were measured to evaluate the salinity tolerance of sea cucumbers.ResultsSignificant correlations in osmotic pressure were observed between coelomic fluid and ambient environmental salinity. In coelomic fluid, Na+ concentration was observed fluctuated during salinity 18 psu and the inflection point presented at the 6 h. The Na+/K+-ATPase activity in respiratory trees indicated the “U-shaped” fluctuant change and the change trend was opposite with the Na+ concentration. The ions (K+, Cl−) concentration decreased and showed the same tendency at salinity 40 psu with salinity 18 psu. The total coelomocytes counts and phagocytosis of coelomic fluid Na+/K+-ATPase activity indicated fluctuating changes under different salinity stress. Histological variation revealed a negative relation between decreasing salt concentration and tissue integrity. Tissue damages were significantly observed in intestines, muscles and tube feet under low salinity environment (18, 23 and 27 psu). The connective tissue in intestines of A. japonicus exposed to 18 and 23 psu damaged and partly separated from the mucosal epithelium. The significant variations occurred in tube feet, which presented the swelling in connective tissue and a fracture in longitudinal muscles under low salinity (18 psu). The morphological change of tube feet showed the shrinkage of connective tissue under high salinity (40 psu). The amount of infusoria in the respiratory trees decreased or even disappeared in salinity treatment groups (18 and 23 psu).ConclusionThe results inferred that osmoconformity and ionoregulation were seen in sea cucumbers, which contributed to understand the salinity regulatory mechanisms of A. japonicus under acute salinity stress.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of acute salinity stress on ion homeostasis, Na+/K+-ATPase and histological structure in sea cucumber Apostichopus japonicus

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, bacterial infectious disease has become a limiting factor that restricts the healthy development of the A. japonicus aquaculture industry (Gowda et al, 2008). There is a growing list of A. japonicus immune effectors with diverse biochemical activities including lysozyme (Tian et al, 2015), complement component 3 , myeloid differentiation factor 88 (MyD88) and tumor necrosis factor receptorassociated factor 6 (TRAF6) (Lu et al, 2013) isolated and characterized. However, knowledge on the relations between GRP78 as well as PDI and immune response with bacterial challenge was scarcely investigated in this important economic species.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of two novel molecular chaperones in bacterial-challenged Apostichopus japonicus

Wang

Shao

Zhang

et al. 2015

Gene

View full text Add to dashboard Cite

“…Among these lysozymes, C‐type lysozyme has the largest proportion in nature, and exists in vertebrates and insects. Till present, many kinds of C‐type lysozyme have been identified in different animals, such as Venerupis philippinarum (Yang et al, ), Trachinotus ovatus (Zhou et al, ), Harmonia axyridis (Beckert et al, ), Apostichopus japonicas (Tian, Liang, Chang, & Song, ), Carassius auratus (Wang et al, ) and Platichthys stellate (Kim & Nam, ), and its function has been studied. The C‐type lysozymes typically possess muramidase activities and chitinase activities, which could cleave the β‐(1,4)‐glycosidic bond of PGN and break down glycosidic bonds or N‐acetylglucosamine linkages of chitin (Fiołka, Ptaszyńska, & Czarniawski, ; Vocadlo, Davies, Laine, & Withers, ).…”

Section: Introductionmentioning

confidence: 99%

Newly identified C‐type lysozyme in Chinese soft‐shelled turtle (Pelodiscus sinensis) exhibits potent antimicrobial activity

Yuan

et al. 2019

Aquac Res

View full text Add to dashboard Cite

Lysozymes play vital roles in humoural immune response against bacterial invasion by its lytic activity. In the present study, a new C‐type lysozyme was identified and characterized from Chinese soft‐shelled turtle Pelodiscus sinensis. The full‐length cDNA of PslysC was of 923 bp, encoding a polypeptide of 148 amino acid residues. The multiple alignments and phylogenetic relationship analysis revealed the highly enzyme‐related conserved residues. The real‐time PCR analysis suggested that PslysC was constitutively expressed in a wide range of tissues with highest level in blood cells and liver. The expression of PslysC could be significantly up‐regulated under Aeromonas jandaei infection and ammonia exposure, while no significant changes were found under Poly I:C infection. The rPslysC protein was expressed in E. coli and purified by Ni‐NTA. The optimal pH and temperature for rPslysC protein lytic activities were determined at pH 7 and 30℃. rPslysC can inhibit the growth of eight kinds of Gram‐negative bacteria, and three kinds of Gram‐positive bacteria. The binding activity of rPslysC to different microbial polysaccharides and microorganism was analysed. The results showed that rPslysC could bind to selected bacteria, and exhibit a strong binding activity to lipopolysaccharide and peptidoglycan, but a weak binding activity to β‐glucan. This suggests that the binding activity might be the major mechanism of action to realize the antibacterial activity. The present study will provide helpful evidence to further understand the innate immunity of P. sinensis, and the interaction mechanisms of C‐type lysozymes with bacterial membranes.

show abstract

Expression of c-type lysozyme gene in sea cucumber (Apostichopus japonicus) is highly regulated and time dependent after salt stress

Cited by 19 publications

References 52 publications

Effect of acute salinity stress on ion homeostasis, Na+/K+-ATPase and histological structure in sea cucumber Apostichopus japonicus

Effect of acute salinity stress on ion homeostasis, Na+/K+-ATPase and histological structure in sea cucumber Apostichopus japonicus

Molecular characterization of two novel molecular chaperones in bacterial-challenged Apostichopus japonicus

Newly identified C‐type lysozyme in Chinese soft‐shelled turtle (Pelodiscus sinensis) exhibits potent antimicrobial activity

Contact Info

Product

Resources

About