Expression of cadherin10, a type II classic cadherin gene, in the nervous system of the embryonic zebrafish

Abstract

Cadherins are cell surface adhesion molecules that play important roles in development of tissues and organs. In this study we analyzed expression pattern of cadherin10, a member of the type II classic cadherin subfamily, in the embryonic zebrafish using in situ hybridization methods. cadherin10 message (cdh10) is first and transiently expressed by the notochord. In the developing nervous system, cdh10 was first detected in a subset of the cranial ganglia, then in restricted brain regions and neural retina. As development proceeds, cdh10 expression domain and/or expression levels increased in the embryonic nervous system. Our results show that cdh10 expression in the zebrafish developing nervous system is both spatially and temporally regulated.

Zebrafish Foxi1 provides a neuronal ground state during inner ear induction preceding the Dlx3b/4b-regulated sensory lineage

“…2A,E). At this stage, both neurod and cdh6 also label progenitors of the anterior lateral line ganglia (gALL) that are closely associated with the delaminated neuroblasts of the otic vesicle (Andermann et al, 2002;Liu et al, 2006). In the absence of Dlx3b/4b, neurod and cdh6 expression are slightly increased or unaffected ( Fig.…”

“…cDNA probes that detect the following genes were used: neurog1 and neurod (Andermann et al, 2002); stm (Söllner et al, 2003); cdh6 and cdh10 (Liu et al, 2006); myo7aa (Ernest et al, 2000); atoh1b (Adolf et al, 2004); tbx1 (Piotrowski et al, 2003); pax2a (Krauss et al, 1991); phox2a and phox2bb (Guo et al, 1999); tlx3b (Langenau et al, 2002); and CreER T2 (Hans et al, 2009). Antibody staining, probe synthesis and in situ hybridization were performed as previously described (Westerfield, 2000).…”

“…In zebrafish, the transcription factors Foxi1 and Dlx3b/4b play pivotal roles during otic induction, and Dlx3b/4b is essential for sensory lineage development (Nissen et Millimaki et al, 2007). To test whether Dlx3b/4b is also required for the neuronal lineage during otic development, we examined neurogenic differentiation (neurod) and cadherin 6 (cdh6) expression (Andermann et al, 2002;Liu et al, 2006). In control embryos at 24 hpf, neurod is expressed in the anterior-ventral region of the otic vesicle and in delaminated neuroblasts, whereas cdh6 is expressed in delaminated neuroblasts only ( Fig.…”

The Order and Place of Neuronal Differentiation Establish the Topography of Sensory Projections and the Entry Points within the Hindbrain

“…For fluorescent in situ hybridization, embryos were first equilibrated in 0.1 M Tris-HCl, pH 8.2, and then the DIG-labeled probe was detected with Fast Red (Roche) dissolved in 0.1 M Tris-HCl, pH 8.2. Probes were as follows: cadh6 and cadh10 (Liu et al, 2006), crestin (Berndt and Halloran, 2006), neuroD (also called neuroD1; Itoh and Chitnis, 2001), slit1a and slit1b (Hutson et al, 2003), snail2 (Thisse et al, 1995), and robo2 and robo3 (Lee et al, 2001).…”

“…The ability of the brain to build an internal representation of the external world based on sensory information, which relies on the establishment of topographic projections, is essential for the accurate transmission of environmental stimuli to processing centers in the brain (for review, see Luo and Flanagan, 2007). For instance, in the inner ear, vestibular or acoustic signals acquired by hair cells are transmitted to bipolar afferent neurons that project axons to the corresponding nuclei in the hindbrain.…”