Expression of CagL from Helicobacter pylori and Preliminary Study of its Biological Function

Wang, Hua; Huang, Shiteng; Zhao, Junlong; Han, Jun; Guan, Xiaohong; Shao, Shihe

doi:10.1007/s12088-012-0341-4

Cited by 8 publications

(8 citation statements)

References 22 publications

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“…A study on 61 isolates from patients with digestive disease in Iran, showed that 96.7% were cagL positive. This report was concordant with the results from Taiwan, where the 98.6% of the patients were cagL positive, but no remarkable association was detected between the cagL genotype and clinical outcomes (P>0.05) (Kwok et al, 2007;Yeh et al, 2011;Wang et al, 2013;Yadegar et al, 2014). These results are consistent with our results in GC group but not in PU group, while the cagL genotype is remarkably and independently associated with the risk of PU in Iran (P=0.021), but no association was found with the risk of GC (P>0.05).…”

Section: Discussionsupporting

confidence: 90%

Helicobacter pylori cag Pathogenicity Island cagL and orf17 Genotypes Predict Risk of Peptic Ulcerations but not Gastric Cancer in Iran

Raei¹,

Latifi‐Navid²,

Zahri³

2015

Asian Pacific Journal of Cancer Prevention

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Background: Gastric cancer (GC) is the third most common cancer regarding mortality in the world. The cag pathogenicity island (PAI) of Helicobacter pylori which contains genes associated with a more aggressive phenotype may involve in the pathogenesis of gastrointestinal disease. We here aimed to examine the associations of cagH, cagL, orf17, and cagG genotypes of H. pylori cag PAI with severe gastrointestinal disease. Materials and Methods: A total of 242 H. pylori strains were genotyped. Histopathological examination and classification of subjects were performed. Results: The frequencies of the cagH, cagL, cagG, and orf17 genotypes were 40/54 (74.1%), 53/54 (98.1%), 38/54 (70.4%), and 43/54 (79.6%), respectively, in patients with peptidic ulceration (PU),while in the control group, the frequencies were 87/147 (59.6%) for cagH, 121/146 (82.9%) for cagL, 109/146 (74.7%) for cagG, and 89/146 (61.0%) for orf17. The results of simple logistic regression analysis showed that the cagL and orf17 genotypes were significantly associated with an increased risk of PU not GC; the ORs (95% CI) were 10.950 (1.446-82.935), and 2.504 (1.193-5.253), respectively. No significant association was found between the cagH and cagG genotypes and the risk of both the PU and the GC in Iran (P>0.05). Finally, multiple logistic regression analysis showed that the cagL genotype was independently and significantly associated with the ageand sex-adjusted risk for PU; the OR (95% CI) was 9.557 (1.219-17.185). Conclusions: We conclude that the orf17 and especially cagL genotypes of H. pylori cag PAI could be factors for risk prediction of PU, but not GC in Iran.

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Section: Discussionsupporting

confidence: 90%

Helicobacter pylori cag Pathogenicity Island cagL and orf17 Genotypes Predict Risk of Peptic Ulcerations but not Gastric Cancer in Iran

Raei¹,

Latifi‐Navid²,

Zahri³

2015

Asian Pacific Journal of Cancer Prevention

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“…It has been demonstrated that more than 85% of the H. pylori strains isolated from patients in India, Malaysia, Taiwan, Iran, and Singapore were cagL positive . No association was found between this genotype and clinical outcomes . These results were consistent with the results of the Raei et al study for GC, but not for PU OR = 10.950) .…”

Section: Discussionsupporting

confidence: 82%

“…14,26,27 No association was found between this genotype and clinical outcomes. [27][28][29][30][31] These results were consistent with the results of the Raei et al study for GC, but not for PU OR = 10.950). 14 In the present study, 78.3% of strains had the cagL + genotype and the results of logistic regression analysis showed a significant reverse association between this genotype and the risk of CGA and NCGA, whether the controls were nontumors or those with NAG.…”

Section: T a B L Esupporting

confidence: 89%

Inverse association of Helicobacter pylori cagPAI genotypes with risk of cardia and non‐cardia gastric adenocarcinoma

et al. 2019

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Iran is a high‐risk country for cardia gastric adenocarcinoma (CGA) in Central Asia, with an incidence rate five times the average global rate, and shows a high infection rate for Helicobacter pylori (69%). The aim was to examine the associations of multiple H. pylori cag PAI genotypes (ie cagH , cagL , cagG , and orf17 ) with the risk of CGA, non‐CGA, and different histological types of GA in Iran. A large number of H. pylori strains (N = 336) were successfully cultured and genotyped. Histopathological evaluations were performed. The analysis showed an inverse association between the cagH + genotype and the risk of CGA and intestinal‐type gastric adenocarcinoma (IGA) (adjusted ORs; 0.312 and 0.283, respectively), where the controls were nontumors. The orf17 + genotype decreased the risk of non‐CGA and diffuse‐type gastric adenocarcinoma (DGA)(adjusted ORs; 0.310 and 0.356, respectively). When the controls were those with nonatrophic gastritis, the cagG + genotype was negatively associated with the risk of CGA, non‐CGA, IGA, and DGA (adjusted ORs; 0.324, 0.366, 0.306, and 0.303, respectively). We did not find such a significant association for the cagL + genotype in multiple logistic regression analysis. Combination of the vacA c2 and cag PAI genotypes further decreased the risk estimates for GAs. This study showed the reverse association of H. pylori cag PAI genotypes— cagH + and cagG + —with the risk of CGA in male patients aged ≥ 55 in Iran. Presence of the vacA c2 genotype in combination with cag PAI genotypes showed strong inverse associations with the risk of CGA and non‐CGA. These findings may reveal a coordinated relationship between the vacA c2 and cag PAI genotypes.

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“…According to the available evidence, CagL antigen binds to a5b1 integrin through its protected domain, arginine-glycine-aspartate (RGD), and translocates the CagA into the host cell, which in turn leads to several change such as focal adhesion formation, activation of epidermal growth receptor, cell spreading, and eventually induction of proinflammatory response [15,16]. In addition, it causes transient hypochlorhydria by inhibiting the binding of a5b1 integrin to ADAM17 metalloprotease [17].…”

Section: Introductionmentioning

confidence: 99%

CagL amino acid (122 and 134) polymorphisms associated with reduction of Helicobacter pylori-related diseases: a systematic review and meta-analysis

Karbalaei,

Keikha

2023

Reviews and Research in Medical Microbiology

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Background: Cytotoxic-associated gene L (CagL) protein is a structural component of the type IV secretion system in Helicobacter pylori that plays a pivotal role in the translocation and secretion of CagA protein. However, the effect of this factor in severe gastroduodenal outcomes has not yet been demonstrated. Methods: In the current study, using databases such as ISI Web of Science, PubMed and Scopus, we systematically investigated the role of cagL gene and its polymorphisms in the pathogenesis of H. pylori. Then, the logical relationship between cagL polymorphisms and frequency of gastrointestinal disorders such as gastric cancer and peptic ulcer disease (PUD) was measured. Results: In total, data from 1071 bacterial strains were evaluated. The prevalence of H. pylori cagL gene in patients with various forms of infections such as nonulcer dyspepsia, PUD, and gastric cancer was measured 81.9% [95% confidence interval (CI): 68.0–90.5], 94.0% (95% CI: 81.4–98.3), and 86.2% (95% CI: 73.8–93.3), respectively. We show that infection with bacteria harboring cagL gene increases the risk of PUD and gastric cancer by 3.7- and 1.7-fold, respectively. Moreover, CagL/CagA/(<2) EPIYA C repeats could increase the risk of severe gastrointestinal disorders by 7.1-fold. Among the all sequence variations of this gene, it was found that only mutations associated with amino acids at positions 122K/N (odds ratio: 0.1) and 134I/V (odds ratio: 0.23) significantly reduced the risk of PUD. Conclusion: Our findings suggest that the presence of the cagL gene could potentially lead to severe gastrointestinal outcomes, particularly PUD and gastric cancer. However, although most polymorphisms of cagL have no a significant relationship with H. pylori-related diseases, but variants 122K/N and 134I/V play a protective role against PUD.

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Expression of CagL from Helicobacter pylori and Preliminary Study of its Biological Function

Cited by 8 publications

References 22 publications

Helicobacter pylori cag Pathogenicity Island cagL and orf17 Genotypes Predict Risk of Peptic Ulcerations but not Gastric Cancer in Iran

Helicobacter pylori cag Pathogenicity Island cagL and orf17 Genotypes Predict Risk of Peptic Ulcerations but not Gastric Cancer in Iran

Inverse association of Helicobacter pylori cagPAI genotypes with risk of cardia and non‐cardia gastric adenocarcinoma

CagL amino acid (122 and 134) polymorphisms associated with reduction of Helicobacter pylori-related diseases: a systematic review and meta-analysis

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