Expression of calbindin-D9k in the early pregnant rat uterus: Effects of RU 486 and correlation to estrogen receptor mRNA

Krisinger, John; Setoyama, Takuya; Leung, Peter C.K.

doi:10.1016/0303-7207(94)90092-2

Cited by 42 publications

(29 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed increase in calbindin-D 9k mRNA expression in early pregnancy followed by a decrease in the mouse uterus is in agreement with the previous reports in the rat [40] and mouse [19], in which a similar pattern was seen. However, the cellular origin of the increase immediately after pregnancy and the following decrease in calbindin-D 9k mRNA in the uterus was not clear.…”

Section: Discussionsupporting

confidence: 94%

“…In pregnant rats, expression of calbindin-D 9k mRNA is relatively high on Day 1 of pregnancy (P1); on P2, P3, and P5 the mRNA decreases to the detection limit of Northern analysis [40]; on P10, the transcripts begin to reappear at levels of about 30% of P1 [40]. In nonpregnant rats, calbindin-D 9k was immunolocalized in the myometrium and endometrial stromal cells, but not in the luminal epithelial cells [41,42], whereas in pregnant rats, the expression was detected predominantly in luminal epithelial cells [42,43].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Complex Regulation of Calcium-Binding Protein D9k (Calbindin-D9k) in the Mouse Uterus During Early Pregnancy and at the Site of Embryo Implantation1

Nie

Wang

et al. 2000

Biology of Reproduction

View full text Add to dashboard Cite

Establishment of receptive endometrium is essential for implantation. Our aim was to identify and characterize genes uniquely regulated at the sites of implantation in mouse uterus by RNA differential display polymerase chain reaction (DDPCR). One of the gene fragments identified was 86% homologous to rat calcium-binding protein D9k (calbindin-D(9k)); the mouse counterpart had not then been cloned, but subsequently an mRNA sequence of mouse calbindin-D(9k) became available in GenBank (accession number: AF028071). This sequence is 99% homologous to the DDPCR-derived gene tag but has a shorter 3' end. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using the sequence of 3' end of the DDPCR product and the 5' end of AF028071, and a full cDNA was obtained. This gene was primarily up-regulated by progesterone, but not by estrogen. It was further increased by the combination of the two steroids. Expression of calbindin-D(9k) was overall increased in the uterus during early pregnancy, but the level was significantly lower in implantation compared to interimplantation sites on Days 4.5 and 5.5 of pregnancy, becoming barely detectable in both sites after Day 6.5. In situ hybridization localized this mRNA predominantly in the luminal epithelium of the pregnant uterus. The complex regulation of calbindin-D(9k) in mouse uterus suggests an important role for this protein during pregnancy.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Complex Regulation of Calcium-Binding Protein D9k (Calbindin-D9k) in the Mouse Uterus During Early Pregnancy and at the Site of Embryo Implantation1

Nie

Wang

et al. 2000

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…However, after attachment (d14), the levels decrease dramatically (25). In rats as in mice, endometrial expression on d1, d2, d3, and d5 of pregnancy is high, and this expression is significantly decreased immediately after initial embryo attachment (12). Thus, in all species examined, CaBP-d9k is elevated before implantation.…”

Section: Discussionmentioning

confidence: 91%

“…CaBP-d28k was originally purified from chicken intestine (8) but is also expressed in kidney and throughout the nervous system (9,10) in species ranging from invertebrates to mammals (11). In the rat, CaBP-d9k has been demonstrated in the uterine stroma and myometrium, with uterine expression decreasing around the time of embryo implantation on day 5 (d5) of pregnancy (12).…”

mentioning

confidence: 99%

Endometrial calbindins are critical for embryo implantation: Evidence fromin vivouse of morpholino antisense oligonucleotides

Luu

Nie

Salamonsen

2004

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The endometrium is receptive to embryo implantation only for a short period in each reproductive cycle: development of receptivity requires alterations in endometrial gene expression. Calbindin (CaBP)-d9k and CaBP-d28k are related proteins containing EF hand motifs that have a high affinity for Ca 2؉ . We previously demonstrated that endometrial expression of CaBP-d9k mRNA is highly regulated during implantation in the mouse. This project aimed to determine the temporal and spatial expression of both CaBP proteins during early pregnancy and to establish whether they are necessary for blastocyst implantation. CaBP-d28k protein, like CaBP-d9k, was up-regulated in the endometrial epithelium just before implantation but disappeared at implantation sites after attachment. By the judicious intrauterine injection of morpholino oligonucleotides (MO) against CaBP-d9k into WT and CaBP-d28k null mice just before implantation, we selectively eliminated one or both CaBPs from the uterine epithelium. Implantation was blocked only when both CaBP-d9k and CaBP-d28k were absent: treated WT mice and untreated CaBP-d28k null mice were fertile. Furthermore, the effect on implantation was highly dependent on the timing of injection of MO. This report examining the function of implantation-related genes in the uterus using MO demonstrates that this technique is a highly effective means to specifically target uterine proteins in vivo. This study provides evidence for an absolute requirement for CaBPs during the early phase of embryo implantation, and thus that regulation of Ca 2؉ availability in the uterine environment of the implanting embryo is critical for successful implantation.

show abstract

“…It has been postulated that uterine CaBP-9k may be involved in controlling myometrial activity related to intracellular calcium levels [18]. The hormonal mechanism controlling the uterine CaBP9k gene is relatively well understood in the rat; for instance, estrogen is known to up-regulate, and progesterone to down-regulate the expression of the CaBP-9k gene in the rat uterus during the estrous cycle and early pregnancy [19][20][21][22]. The C a B P -9 k m R N A e x p r e ss i o n o f e st r o g e n i c compounds in a dose-and time-dependent manner has been assessed by Dot and Northern blot analyses.…”

Section: Rat Modelsmentioning

confidence: 99%

The Biomarker and Endocrine Disruptors in Mammals

Choi

Jeung²

2003

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. The compounds that bind steroid hormone receptors including estrogen receptors (ERs), progesterone receptor (PR) or androgen receptor (AR), and induce or modulate a steroid hormone receptor-mediated response could be defined as endocrine disruptors (EDs). Currently, there are no standard methods to determine whether a chemical is an endocrine disruptor or not. Most results of in vitro and in vivo data are derived from assays that measure estrogenic activity, thus fewer data are available from assays that measure androgenic and progestogenic activities. In this review, we introduce a novel in vivo model to detect EDs using immature rats in the induction of Calbindin-D9k (CaBP-9k) mRNA and protein by estrogenic compounds. In addition, we summarize other biomarkers and screening methods for EDs in mammals to describe the usefulness of indicated biomarkers, although mammalian models are very few based on experimental findings.

show abstract

Expression of calbindin-D9k in the early pregnant rat uterus: Effects of RU 486 and correlation to estrogen receptor mRNA

Cited by 42 publications

References 23 publications

Complex Regulation of Calcium-Binding Protein D9k (Calbindin-D9k) in the Mouse Uterus During Early Pregnancy and at the Site of Embryo Implantation1

Complex Regulation of Calcium-Binding Protein D9k (Calbindin-D9k) in the Mouse Uterus During Early Pregnancy and at the Site of Embryo Implantation1

Endometrial calbindins are critical for embryo implantation: Evidence fromin vivouse of morpholino antisense oligonucleotides

The Biomarker and Endocrine Disruptors in Mammals

Contact Info

Product

Resources

About