Expression of CD133 in SW620 colorectal cancer cells is modulated by the microenvironment

Yang, Zhili; Wang, Zhigang; Fan, Youben; Zheng, Qi

doi:10.3892/ol.2012.694

Cited by 13 publications

(9 citation statements)

References 32 publications

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“…They found that the frequency of tumor cells positive for stem cell-like cell markers and markers of more differentiated cells varied according to the tumor subtype and histologic stage [61]. Furthermore, a recent study found that CD133 expression in colorectal cancer cells is modulated by the microenvironment [62]. In addition to colorectal cancer, there are other cancers in which the expression of CD133 is regulated, such as gliomas and prostate cancer.…”

Section: Surface Markersmentioning

confidence: 99%

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Duan

Qiu

et al. 2013

Stem Cells and Development

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Cancer stem cells (CSCs) constitute a subpopulation of cancer cells that have the potential for self-renewal, multipotent differentiation, and tumorigenicity. Studies on CSC biology and CSC-targeted therapies depend on CSC isolation and/or enrichment methodologies. Scientists have conducted extensive research in this field since John Dick's group successfully isolated CSCs based on the expression of the CD34 and CD38 surface markers. Progress in CSC research has been greatly facilitated by the enrichment and isolation of these cells. In this review, we summarize the current strategies used in our and other laboratories for CSC isolation and enrichment, including methods based on stem cell surface markers, intracellular enzyme activity, the concentration of reactive oxygen species, the mitochondrial membrane potential, promoter-driven fluorescent protein expression, autofluorescence, suspension/adherent culture, cell division, the identification of side population cells, resistance to cytotoxic compounds or hypoxia, invasiveness/adhesion, immunoselection, and physical property. Although many challenges remain to be overcome, it is reasonable to believe that more reliable, efficient, and convenient methods will be developed in the near future.

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Section: Surface Markersmentioning

confidence: 99%

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Duan

Qiu

et al. 2013

Stem Cells and Development

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“…In addition, hypoxia-induced CD133 expression has been reported in human lung cancer cell lines (12). It has been reported that in SW620 colorectal cancer cells, CD133 expression changes depending on the type of the microenvironment (25). Under the low-nutrient culture condition, CD133-negative SW620 colorectal cancer cells re-expressed CD133.…”

Section: Introductionmentioning

confidence: 99%

Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids

Ohnishi

Tani

Bando

et al. 2014

International Journal of Oncology

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Recent studies showed that the stemness of cancer stem cells is maintained under a hypoxic microenvironment. However, the relationship of the hypoxic microenvironment in a three-dimensional cell mass and the induction of cancer stem cell-like phenotype is not well known. We examined the relationship between CD133 expression and the hypoxic microenvironment using glioblastoma spheroids formed with the T98G cell line. CD133(AC133)- and HIF-1α-positive cells were observed in the marginal region of the central hypoxic area positive for HIF-1α 10 days after plating T98G cells. CD133(AC133)-positive cells were positive for nestin. Quantitative PCR analysis showed that the CD133 expression level is not different in spheroids during the tested period after spheroid formation, indicating that post-translational regulation of the CD133 protein mediates positivity to CD133(AC133). When spheroids were trypsinized and the dissociated cells were cultured under the adherent monolayer conditions, the CD133(AC133)-positive cells gradually disappeared. These results show that CD133(AC133)-positive cells, which may incline toward undifferentiated cells because of nestin positivity, are plastically induced under the different culture conditions, spheroid and monolayer. In this plasticity, HIF-1α is involved in the induction and maintenance of CD133(AC133)-positive cells. Spheroids as an in vitro tumor model are useful to study the dynamic changes in the tumor cell phenotype in the different cell microenvironments.

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“…Ma et al first identified the presence of 1.3% to 13.6% of CD133 + cells in 35 individual HCC specimens by flow cytometry that generated tumors in SCID/Beige mice in serial transplantations [7]. CD133-positive population is generally in a relative constant percentage in cell lines and tissues but increased in malignant transformation, which suggest that the transmembrane pentaspan protein may play an essential role in cell metabolism and survival [8]–[10]. Characterizing CD133 functions in tumor and incorporating these findings into cancer drug discovery might lead to better therapeutic approaches [11].…”

Section: Introductionmentioning

confidence: 99%

CD133/Prominin-1-Mediated Autophagy and Glucose Uptake Beneficial for Hepatoma Cell Survival

et al. 2013

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CD133/Prominin-1 is a pentaspan transmembrane protein that has been frequently used as a biomarker for cancer stem cells, although its biological function is unclear. The aim of our study was to explore the intrinsic functions of CD133 membrane protein in hepatoma cells during autophagy, apoptosis, tumorigenesis and cell survival through expression or downregulation of CD133. In this study, CD133 was found to be dynamically released from plasma membrane into cytoplasm in both of complete medium(CM) and low glucose medium (LGM), and LGM promoted this translocation. Expression of CD133 enhanced autophagic activity in LGM, while silencing CD133 attenuated this activity in HCC LM3 and Huh-7 cells, suggesting that CD133 is associated with autophagy. Immunofluorescence and time-lapsed confocal techniques confirmed that CD133 was associated with autophagy marker, microtubule-associated protein light chain3 (LC3) and lysosome marker during the glucose starvation. We further found that Huh-7 cells with stable expression of shCD133 (Huh-7sh133) impaired the ability of cell proliferation and formation of xenograft tumors in the NOD/SCID mice. Although loss of CD133 did not affect the rates of glucose uptake in Huh-7con and Huh-7sh133 cells under the CM, Huh-7sh133 cells obviously died fast than Huh-7con cells in the LGM and decreased the rate of glucose uptake and ATP production. Furthermore, targeting CD133 by CD133mAb resulted in cell death in HepG2 cells, especially in the LGM, via inhibition of autophagic activity and increase of apoptosis. The results demonstrated that CD133 is involved in cell survival through regulation of autophagy and glucose uptake, which may be necessary for cancer stem cells to survive in tumor microenvironment.

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Expression of CD133 in SW620 colorectal cancer cells is modulated by the microenvironment

Cited by 13 publications

References 32 publications

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids

CD133/Prominin-1-Mediated Autophagy and Glucose Uptake Beneficial for Hepatoma Cell Survival

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