Expression of CD203c on basophils as a marker of immunoglobulin E-mediated<scp>l</scp>-asparaginase allergy

Hino, Moeko; Shimojo, Naoki; Ochiai, Hirotaka; Inoue, Yuzaburo; Ando, Kiyohiro; Chikaraishi, Koji; Ota, Setsuo; Okimoto, Yuri; Sunami, Shosuke; Nakamura, Ryosuke; Teshima, Reiko; Sato, Yasunori; Kohno, Yoichi

doi:10.3109/10428194.2013.794944

Cited by 9 publications

(10 citation statements)

References 18 publications

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“…( 9 ) In addition, it has been reported that CD203c is a useful marker for allergies induced by amoxicillin and (L)-asparaginase. ( 10 , 11 ) We recently confirmed that patients with a history of CBDCA-induced severe HR showed an increase in CD203c-positive basophils compared with patients without HR. ( 12 ) In addition, all five patients with grade 2 or higher HR showed increased CD203c on basophils during the months following the onset of HR, whereas no such increase was not observed in the previous courses of CBDCA.…”

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confidence: 68%

Carboplatin‐induced severe hypersensitivity reaction: Role of IgE‐dependent basophil activation and FcεRI

et al. 2014

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Basophil activation was observed in patients with a history of carboplatin-induced severe hypersensitivity reaction (HR). However, the precise mechanism by which carboplatin induces basophil activation and the associated surrogate markers remains to be elucidated. To investigate whether IgE-dependent mechanisms, including the overexpression of FcεRI, participate in carboplatin-induced basophil activation, 13 ovarian cancer patients were enrolled: 5 with a history of carboplatin-induced severe hypersensitivity reaction within the past 2 years, and 8 with no such history. The expression levels of FcεRI, IgE, and CD203c on basophils were measured using a flow cytometer. Immunoglobulin E-dependent basophil activation was evaluated by testing for IgE passive sensitization using lactic acid, and by testing for phosphatidylinositol 3-kinase inhibition, using wortmannin. In three patients positive for carboplatin hypersensitivity, pretreatment with wortmannin almost completely inhibited carboplatin-induced basophil activation (P < 0.05). In a healthy control subject, whose own IgE showed no response to carboplatin, acquired reactivity to carboplatin when exposed to plasma from patients positive for carboplatin hypersensitivity. This did not occur when the same experiment was carried out using plasma from the patients negative for carboplatin hypersensitivity. Moreover, pretreatment with omalizumab, a monoclonal anti-IgE antibody, almost completely blocked carboplatin-induced basophil activation in the plasma of patients positive for carboplatin hypersensitivity. On further investigation, the HR-positive group had significantly higher levels of FcεRI compared with the negative group (P < 0.05). In conclusion, an IgE-dependent mechanism incorporating FcεRI overexpression participates in carboplatin-induced severe HR. These results establish the relevance of monitoring the pharmacodynamic changes of basophils to prevent carboplatin-induced severe HR.

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confidence: 68%

Carboplatin‐induced severe hypersensitivity reaction: Role of IgE‐dependent basophil activation and FcεRI

et al. 2014

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“…Recently, it has been shown that BAT is a useful marker for identifying l -asparaginase allergy because of its high sensitivity and specificity, and combining the BAT with an l -asparaginase-specific IgG assay is the most accurate method of identifying l -asparaginase allergy. 30 …”

Section: Discussionmentioning

confidence: 99%

Do immunoglobulin G and immunoglobulin E anti- l -asparaginase antibodies have distinct implications in children with acute lymphoblastic leukemia? A cross-sectional study

Galindo-Rodríguez

Jaime‐Pérez

Salinas-Carmona

et al. 2017

Revista Brasileira de Hematologia e Hemoterapia

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Backgroundl-Asparaginase is essential in the treatment of childhood acute lymphoblastic leukemia. If immunoglobulin G anti-l-asparaginase antibodies develop, they can lead to faster plasma clearance and reduced efficiency as well as to hypersensitivity reactions, in which immunoglobulin E can also participate. This study investigated the presence of immunoglobulin G and immunoglobulin E anti-l-asparaginase antibodies and their clinical associations.MethodsUnder 16-year-old patients at diagnosis of B-cell acute lymphoblastic leukemia confirmed by flow cytometry and treated with a uniform l-asparaginase and chemotherapy protocol were studied. Immunoglobulin G anti-l-asparaginase antibodies were measured using an enzyme-linked immunosorbent assay. Intradermal and prick skin testing was performed to establish the presence of specific immunoglobulin E anti-l-asparaginase antibodies in vivo. Statistical analysis was used to investigate associations of these antibodies with relevant clinical events and outcomes.ResultsFifty-one children were studied with 42 (82.35%) having anti-l-asparaginase antibodies. In this group immunoglobulin G antibodies alone were documented in 10 (23.8%) compared to immunoglobulin E alone in 18 (42.8%) patients. Immunoglobulin G together with immunoglobulin E were simultaneously present in 14 patients. Children who produced exclusively immunoglobulin G or no antibodies had a lower event-free survival (p-value = 0.024). Eighteen children (35.3%) relapsed with five of nine of this group who had negative skin tests suffering additional relapses (range: 2–4), compared to none of the nine children who relapsed who had positive skin tests (p-value < 0.001).ConclusionChildren with acute lymphoblastic leukemia and isolated immunoglobulin G anti-l-asparaginase antibodies had a higher relapse rate, whereas no additional relapses developed in children with immunoglobulin E anti-l-asparaginase antibodies after the first relapse.

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“…Upon basophil activation, these two markers are commonly upregulated with similar kinetics, but they have distinct characteristics from each other. CD63 has been better validated for drug allergies; however, CD203c is increasingly utilized in recent studies [ 15 , 23 , 34 , 35 , 48 , 51 , 60 ]. Upon anaphylactic stimulation, there is degranulation that causes CD63 to appear at the cell surface during the process of fusion of main granules with plasma membranes [ 2 ].…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies [ 60 - 64 ] examined the outcome of BAT in patients with hypersensitivities to antineoplastic, biologic agents, or other drugs ( Table 6 ). L -Asparaginase allergies were assessed using CD203c expression and were found to have high sensitivity (75%) and negative predictive value (96%) [ 60 ]. One case study also reported the potential utility of BAT in cisplatin hypersensitivity [ 65 ].…”

Section: Clinical Applicationsmentioning

confidence: 99%

Recent applications of basophil activation tests in the diagnosis of drug hypersensitivity

Song

Chang

2013

Asia Pacific Allergy

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Immediate-type drug hypersensitivity is an increasingly significant clinical issue; however, the diagnosis is frequently hindered due to lack of safe and precise diagnostic tests. Flow cytometry-assisted basophil activation test is a safe in vitro diagnostic tool for assessing basophil activation upon allergen stimulation. In this review, we have summarized current literature on the diagnostic utilities, new indications, and methodological aspects of the basophil activation test for the diagnosis of drug hypersensitivity.

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Expression of CD203c on basophils as a marker of immunoglobulin E-mediatedl-asparaginase allergy

Cited by 9 publications

References 18 publications

Carboplatin‐induced severe hypersensitivity reaction: Role of IgE‐dependent basophil activation and FcεRI

Carboplatin‐induced severe hypersensitivity reaction: Role of IgE‐dependent basophil activation and FcεRI

Do immunoglobulin G and immunoglobulin E anti- l -asparaginase antibodies have distinct implications in children with acute lymphoblastic leukemia? A cross-sectional study

Recent applications of basophil activation tests in the diagnosis of drug hypersensitivity

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