Expression of CD34 and L-Selectin on Human Corneal Keratocytes

Joseph, Annie; Hossain, Parwez; Jham, Seem A.; Jones, Rhodri E.; Tighe, Patrick J.; McIntosh, Richard S.; Dua, Harminder S.

doi:10.1167/iovs.02-0999

Cited by 70 publications

(67 citation statements)

References 16 publications

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“…However, the current minimal criteria for MSC are not ideal for assessing the phenotype of CSSC, as one criterion states that MSC should not express CD34. Nevertheless, our group has showed CD34 is a well-established marker for quiescent keratocytes in vivo [14,15], and CD34 has been linked ABCG2 is a molecular determinant of the side population phenotype that is characteristic of stem cells, and has been used previously to identify CSSC [26,44]. ABCG2 is expressed by a wide variety of stem cells including haematopoietic stem cells [45], embryonic stem cells [46], neural stem cells [47], and perhaps most relevantly limbal epithelial stem cells [48].…”

Section: Discussionmentioning

confidence: 99%

“…Markers traditionally used to identify the keratocyte phenotype include aldehyde dehydrogenase (ALDH), keratocan, CD133 and, as originally identified by our group, CD34 [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…While this may prove more easily translatable to the clinic compared to serum, the addition of recombinant growth factors, both in research and clinically, can be costly. In addition, keratocytes express CD34 in vivo [14,15], a marker commonly associated with haematopoietic stem cells, which might suggest that the use of culture media traditionally used to support cells of haematopoietic origin might be suitable.…”

Section: Introductionmentioning

confidence: 99%

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Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

et al. 2015

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(2015) Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17 (12). pp. 1706 -1722 . ISSN 1477 -2566 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/31287/1/Submission%20Manuscript.pdf Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the University of Nottingham End User licence and may be reused according to the conditions of the licence. For more details see: http://eprints.nottingham.ac.uk/end_user_agreement.pdf A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. However, the optimal culture conditions are disputed and few direct media comparisons have been performed. In this report, we evaluated several media types to identify the optimal for inducing an in vitro stem cell phenotype. Discussion: Culture medium can significantly influence CSSC phenotype. SCM produced a cell phenotype closest to that of a pluripotent stem cell, and we considerate to be the most appropriate for development as a clinical grade medium for the production of CSSC phenotypes suitable for cell therapy. Methods

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Section: Discussionmentioning

confidence: 99%

“…Markers traditionally used to identify the keratocyte phenotype include aldehyde dehydrogenase (ALDH), keratocan, CD133 and, as originally identified by our group, CD34 [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

et al. 2015

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“…CD34 + non‐hematopoietic lineage cells also represent a distinct subset of cells with enhanced progenitor activity, such as muscle satellite cells, interstitial cells, epithelial progenitors and CSKs 51. The CD34 + expressing cells could thus represent the quiescent CSKs or stromal progenitors,52 whereas CD34 − cells are the activated keratocytes not yet fully reverted to the quiescent condition 53. The possibility of fibroblast transition is excluded by the absence of Thy1 expression 37, 38.…”

Section: Discussionmentioning

confidence: 99%

Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy

Yam

Teo

Setiawan

et al. 2018

J Cellular Molecular Medi

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Corneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest. We harvested PDL cells from young adult teeth extracted because of non‐functional or orthodontic reason and differentiated them towards CSK phenotype using a two‐step protocol with spheroid formation followed by growth factor and cytokine induction in a stromal environment (human amnion stroma and porcine corneal stroma). Our results showed that the PDL‐differentiated CSK‐like cells expressed CSK markers (CD34, ALDH3A1, keratocan, lumican, CHST6, B3GNT7 and Col8A2) and had minimal expression of genes related to fibrosis and other lineages (vasculogenesis, adipogenesis, myogenesis, epitheliogenesis, neurogenesis and hematogenesis). Introduction of PDL spheroids into the stroma of porcine corneas resulted in extensive migration of cells inside the host stroma after 14‐day organ culture. Their quiescent nature and uniform cell distribution resembled to that of mature CSKs inside the native stroma. Our results demonstrated the potential translation of PDL cells for regenerative corneal cell therapy for corneal opacities.

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“…CD34 is expressed in stromal keratocytes but not in corneal epithelium, endothelium, or human corneal stromal stem cells in vitro [16][17][18]. CD34 expression is lost in keratocytes that have become myofibroblastic or fibroblastic in culture [16,19]. In addition, the cultured cells stained positively for chondroitin sulfate (Fig.…”

Section: Isolation and Transplantation Of Hfksmentioning

confidence: 98%

Human Fetal Keratocytes Have Multipotent Characteristics in the Developing Avian Embryo

Chao

Bronner‐Fraser

Lwigale

2013

Stem Cells and Development

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The human cornea contains stem cells that can be induced to express markers consistent with multipotency in cell culture; however, there have been no studies demonstrating that human corneal keratocytes are multipotent. The objective of this study is to examine the potential of human fetal keratocytes (HFKs) to differentiate into neural crest-derived tissues when challenged in an embryonic environment. HFKs were injected bilaterally into the cranial mesenchyme adjacent to the neural tube and the periocular mesenchyme in chick embryos at embryonic days 1.5 and 3, respectively. The injected keratocytes were detected by immunofluorescence using the human cell-specific marker, HuNu. HuNu-positive keratocytes injected along the neural crest pathway were localized adjacent to HNK-1-positive migratory host neural crest cells and in the cardiac cushion mesenchyme. The HuNu-positive cells transformed into neural crest derivatives such as smooth muscle in cranial blood vessels, stromal keratocytes, and corneal endothelium. However, they failed to form neurons despite their presence in the condensing trigeminal ganglion. These results show that HFKs retain the ability to differentiate into some neural crest-derived tissues. Their ability to respond to embryonic cues and generate corneal endothelium and stromal keratocytes provides a basis for understanding the feasibility of creating specialized cells for possible use in regenerative medicine.

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Expression of CD34 and L-Selectin on Human Corneal Keratocytes

Cited by 70 publications

References 16 publications

Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy

Human Fetal Keratocytes Have Multipotent Characteristics in the Developing Avian Embryo

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