Expression of CD44v6 in advanced gastric cancer and its relationship to hematogenous metastasis and long‐term prognosis

Yamaguchi, Akio; Goi, Takanori; Yu, Jiren; Hirono, Yasuo; Ishida, Makoto; Iida, Atsuo; Kimura, Toshihisa; Takeuchi, Kazuo; Katayama, Kanji; Hirose, Kazuo

doi:10.1002/jso.10082

Cited by 51 publications

(31 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Its structure and function FIGURE 2: Kaplan-Meier survival curve for the patients with gastric cardia adenocarcinoma according to whether they were positive (n = 15) or negative (n = 27) for lymph node micrometastasis (LNM); the between-group 2-year survival rate differed significantly (63.6% versus 95.6%, P = 0.011) CD44v6 has been shown to be related to disease progression, lymph node metastasis and prognosis in gastric carcinoma. 17,18 In the present study, micrometastasis indicated by the presence of CK19 with or without CD44v6 was seen in 33 lymph nodes (9.5%) from 15 patients (33.3%); this is consistent with the results of Yun et al 10 We found that lymph node micrometastasis was correlated with the depth of invasion and Lauren classification: the micrometastasis rate was much higher in diffuse than in intestinal cancers. This finding may be explained by the association of an Ecadherin gene mutation with diffuse-type gastric cancer, which results in decreased cell adhesion and easily separated and aggressive tumour cells.…”

Section: Discussionsupporting

confidence: 92%

Detection and Clinical Significance of Lymph Node Micrometastasis in Gastric Cardia Adenocarcinoma

Zhang

Chen

et al. 2012

J Int Med Res

View full text Add to dashboard Cite

OBJECTIVES: Lymph node micro metastasis was investigated in gastric cardia adenocarcinoma (GCA) patients without lymph node metastasis on routine pathological examination. The relationship between micrometastasis and clinicopathological features was also evaluated. METHODS: A total of 349 lymph nodes were obtained from 45 patients with GCA. Micrometastases were detected by immunohistochemical staining for the markers cytokeratin 19 (CK19) and CD44 variant 6 (CD44v6). RESULTS: A total of 33 lymph nodes (9.5%) from 15 patients (33.3%) were positive for CK19. Of these, 27 lymph nodes (7.7%) from 12 patients (26.7%) were also positive for CD44v6. Micrometastasis was significantly related to depth of tumour invasion and Lauren classification (intestinal or diffuse). The recurrence rate was significantly higher and 2-year survival rate significantly lower in patients with than in those without lymph node micrometastasis, showing the necessity of detecting micrometastasis in GCA patients who test negative for lymph node metastasis on routine examination. CONCLUSION: CK19 and CD44v6 were shown to be good markers for micrometastasis detection.

show abstract

Section: Discussionsupporting

confidence: 92%

Detection and Clinical Significance of Lymph Node Micrometastasis in Gastric Cardia Adenocarcinoma

Zhang

Chen

et al. 2012

J Int Med Res

View full text Add to dashboard Cite

show abstract

“…The results showed that the expression rate of CD44 in tumoral tissue reached 65% in contrast to no expression of this molecule in the adjacent normal mucosa. CD44 was more commonly expressed in the intestinal-type tumor than in diffusetype tumor, indicating that CD44 expression was related to the histological subtype of tumor, which is in agreement with previous studies [15,16] . In addition, a correlation was found between CD44 expression and tumor grade.…”

Section: Discussionsupporting

confidence: 92%

“…A study showed that the intestinaltype was more frequently CD44s-and CD44v6-positive than the diffuse-type tumor [15] , although the reactivity to these two antibodies did not correlate with histopathological and clinical prognostic factors in intestinal-type carcinoma [31] . More recently, Yamaguchi et al [16] found that the expression of CD44v6 protein was significantly higher in differentiated adenocarcinoma than in diffuse-type carcinoma. On the other hand, Saito et al [29] observed that CD44v6 appeared to play an important role in the invasion and metastasis of diffusetype gastric carcinoma, but not in that of intestinal-type gastric carcinoma, and also demonstrated a significant correlation between CD44v6 expression and poor prognosis of diffuse-type gastric carcinoma.…”

Section: Discussionmentioning

confidence: 98%

Expression of cell adhesion molecule CD44 in gastric adenocarcinoma and its prognostic importance

Ghaffarzadehgan¹,

Jafarzadeh²,

Raziee³

et al. 2008

WJG

View full text Add to dashboard Cite

used for histological evaluation, including the type (Lauren's classification) and grading of the tumor. The expression of CD44 in the gastric adenocarcinoma mucosa and the adjacent mucosa were determined by immunohistochemistry. The survival analysis was obtained using the Kaplan-Meier test. RESULTS: Of 100 patients, 74 (74%) patients were male. The tumors were categorized as intestinal type (78%) or diffuse type (22%). Sixty-five percent of patients were CD44-positive. CD44 expression was not detected in normal gastric mucosa. Rather, CD44 was more commonly expressed in the intestinal subtype (P = 0.002). A significant relation was seen between the grade of tumor and the expression of CD44 (P = 0.014). The survival analysis showed a poor prognosis of patients with CD44-positive tumors (P = 0.008); and this was more prominent in the intestinal (P = 0.001) rather than diffuse type. CONCLUSION: Cell adhesion molecule CD44 is highly expressed in gastric adenocarcinoma. CD44 expression is correlated with a poor prognosis in patients with the intestinal type of gastric adenocarcinoma. CD44 can, therefore, be utilized as a prognostic marker for this group of patients. Abstract AIM: To evaluate the relation of cluster of differentiation 44 (CD44) expression with clinicopathological features of gastric adenocarcinoma, and also its effect on prognosis with an emphasis on the differences between intestinal and diffuse types. METHODS: From 2000 to 2006, 100 patients with gastric adenocarcinoma, who had undergone total or subtotal gastrectomy without any prior treatment, were studied. Haematoxylin & eosin (HE) staining was

show abstract

“…Joo et al [26,27] obtained almost the same results as ours. But Yamaguchi et al [28] tended to consider CD44v6 protein to have an important role in hematogenous metastasis of gastric carcinomas.…”

Section: A B C D E Fmentioning

confidence: 99%

Expression of heparanase gene, CD44v6, MMP-7 andnm23protein and their relationship with the invasion and metastasis of gastric carcinomas

Chen

Zhan²,

He³

et al. 2004

WJG

View full text Add to dashboard Cite

AIM:To explore the expression of heparanase gene, CD44v6, MMP-7 and nm23 proteins in human gastric carcinoma as well as their relationship with the clinicopathological factors. METHODS:Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the expressions of heparanase mRNA in 43 human gastric carcinomas and 10 adjacent normal gastric tissues. Streptavidin-peroxidase (S-P) two step method was used to measure the immunohistochemical expression of CD44v6, MMP-7 and nm23 protein in 43 human gastric carcinomas. RESULTS:The expression of heparanase gene was positive in 29 cases of gastric cancer with a positive rate of 67.4%, which was significantly higher than those in adjacent normal gastric tissues (P<0.05). The heparanase mRNA expression was significantly related to advanced stage of disease, serosal infiltration, lymph node metastasis and size of tumors (P<0.05), but not related to tumor location, gross and histological types of the cancer, peritoneal dissemination and liver metastasis (P>0.05). The positive rate of CD44v6, nm23 and MMP-7 proteins was 76.7%, 37.2% and 60.5%, respectively. The CD44v6 protein expression was significantly related to serosal infiltration, lymph node metastasis and TNM stage of disease (P<0.05). The nm23 protein expression was significantly related to the tumor gross appearance, lymph node and peritoneal metastasis (P<0.05). The MMP-7 protein expression was significantly related to serosal infiltration and TNM stage of disease (P<0.05). In an attempt to measure the association between these agents, we found that the expression of heparanase mRNA had significantly negative correlation with the expression of CD44v6 and MMP-7 protein (P<0.05), the expression of MMP-7 protein had significantly positive correlation with the expression of CD44v6 protein (r=0.568, P<0.05), the expression of MMP-7 protein had no correlation with the expression of nm23 protein (P>0.05), and the expression of nm23 protein had no correlation with the expression of CD44v6 protein (P>0.05). CONCLUSION:Despite their different mechanisms of action, heparanase, CD44v6 and nm23 may play important roles in the invasive infiltration and lymph node metastasis in gastric carcinomas. Instead of metastatic spreading, MMP-7 may be just related to the invasion of gastric carcinomas. However, co-detection of these factors may be important to predict metastatic spreading in such patients.

show abstract

Expression of CD44v6 in advanced gastric cancer and its relationship to hematogenous metastasis and long‐term prognosis

Abstract: CD44v6 protein may have an important role in hematogenous metastasis, and may be a biologic marker of prognostic significance in differentiated type gastric cancers.

Cited by 51 publications

References 17 publications

Detection and Clinical Significance of Lymph Node Micrometastasis in Gastric Cardia Adenocarcinoma

Detection and Clinical Significance of Lymph Node Micrometastasis in Gastric Cardia Adenocarcinoma

Expression of cell adhesion molecule CD44 in gastric adenocarcinoma and its prognostic importance

Expression of heparanase gene, CD44v6, MMP-7 andnm23protein and their relationship with the invasion and metastasis of gastric carcinomas

Contact Info

Product

Resources

About