Expression of CD45 isoforms by fresh and activated human γδ T lymphocytes and natural killer cells

Braakman, Eric; Sturm, Els; Vijverberg, Kitty; Krimpen, Brigitte A. van; Gratama, Jan W.; Bolhuis, R. L. H.; Moretta, L

doi:10.1093/intimm/3.7.691

Cited by 36 publications

(29 citation statements)

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“…It has been described that the majority of Vd2 + cells express CD45RO + , whereas only a minority of Vd1 + cells are CD45RO + [34,35]. Thus, the presence of a dominant Vd1 + subset of gd + T cells in these patients might explain the lower percentage of CD45RO + gd + T cells in these groups.…”

Section: Discussionmentioning

confidence: 87%

Lymphocyte activation and subset redistribution in the peripheral blood in acute malaria illness: distinct γδ+ T cell patterns in Plasmodium falciparum and P. vivax infections

Worku

Björkman

Troye‐Blomberg

et al. 1997

Clinical and Experimental Immunology

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SUMMARYLymphocyte subset distributions and activation in the peripheral blood were studied in 39 patients with acute malaria and 16 healthy controls from Addis Ababa and Nazareth, Ethiopia. As confirmed by polymerase chain reaction (PCR), 15 patients were infected with Plasmodium falciparum (Pf ), 17 with P. vivax (Pv) and seven were double-infected (Di) with both Pf and Pv. Three-colour flow cytometry was used for phenotyping. Total leucocyte and lymphocyte counts were lower in malaria patients than in controls. The T cell count was reduced in Pf patients, while in the Pv and Di patients there was a reduction in the natural killer (NK) cell count. The CD4/CD8 ratio remained unchanged. gd + T cells were significantly elevated in Pf and Di patients, but not in Pv patients. The increase in gd + T cells was mostly due to an increase in Vd1 + cells. Analyses of cellular activation indicated by the expressions of CD25 and HLA-DR revealed significantly higher numbers of activated CD3 + cells, including gd + T cells, in all patient groups compared with controls. Our results thus indicate that in acute malaria illness there is a complex pattern of change in lymphocyte subset distribution and activation, including gd + T cells. These patterns in Pf infection seem to be distinct from those in Pv infection.

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Section: Discussionmentioning

confidence: 87%

Lymphocyte activation and subset redistribution in the peripheral blood in acute malaria illness: distinct γδ+ T cell patterns in Plasmodium falciparum and P. vivax infections

Worku

Björkman

Troye‐Blomberg

et al. 1997

Clinical and Experimental Immunology

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“…The levels of CD16 expression on circulating NK cells have been shown to correlate with a more activated phenotype (47). However, siglec-7 ϩ NK cells completely lacked CD45RO, a marker that is normally associated with recent activation (48). This observation raises the interesting possibility that siglec-7 can become down-regulated following NK cell activation.…”

Section: Discussionmentioning

confidence: 94%

Identification and Characterization of a Novel Siglec, Siglec-7, Expressed by Human Natural Killer Cells and Monocytes

Nicoll¹,

Ni²,

Liu³

et al. 1999

Journal of Biological Chemistry

236

219

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We describe the characterization of sialic acid-binding Ig-like lectin-7 (siglec-7), a novel member of the siglec subgroup of the immunoglobulin superfamily. A full-length cDNA encoding siglec-7 was isolated from a human primary dendritic cell cDNA library. Siglec-7 is predicted to contain three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs. Overall, siglec-7 exhibited a high degree of sequence similarity to genes encoding CD33 (siglec-3), siglec-5, OBBP1/siglec-6, and OBBP-like protein and mapped to the same region on chromosome 19q13.3. When siglec-7 was expressed on COS or Chinese hamster ovary cells, it was able to mediate high levels of sialic acid-dependent binding to human erythrocytes and soluble sialoglycoconjugates, suggesting that it may be involved in cell-cell interactions. Among human peripheral blood leukocytes, siglec-7 was found to be present at low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8 ؉ T cells. Immunoprecipitation experiments indicated that siglec-7 is expressed as a monomer of ϳ65 kDa.

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“…Yet, these TCRyISS cells display differential expression of CD45RO. The vast majority of V62+ cells express CD45R0, while only a minority of V61+ cells are CD45RO positive (30,31). In other words, a decrease in the proportion of circulating Val+ TCRyIS cells leads to an increase in the proportion of CD45RO-t TCRyIS cells.…”

Section: Discussionmentioning

confidence: 99%

Distinct immunologic features of finnish Sjögren's syndrome patients with HLA alleles DRB10301, DQA10501, and DQB1*0201. Alterations in circulating T cell receptor γ/δ subsets

Kerttula

Collin

Polvi

et al. 1996

Arthritis & Rheumatism

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Objective. To determine whether there were differences in the circulating T lymphocyte subsets or clinical features of patients with primary Sjogren's syndrome (SS) who were positive for different HLA alleles.Methods. Two-and three-color flow cytometry analyses were performed, using a whole blood lysing method.Results Submitted for publication Octoher 4, 1995; accepted in revised form April 16, 1996. autoimmune disorders in patients with the HLA-DR3 haplotype.

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Expression of CD45 isoforms by fresh and activated human γδ T lymphocytes and natural killer cells

Cited by 36 publications

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Lymphocyte activation and subset redistribution in the peripheral blood in acute malaria illness: distinct γδ+ T cell patterns in Plasmodium falciparum and P. vivax infections

Lymphocyte activation and subset redistribution in the peripheral blood in acute malaria illness: distinct γδ+ T cell patterns in Plasmodium falciparum and P. vivax infections

Identification and Characterization of a Novel Siglec, Siglec-7, Expressed by Human Natural Killer Cells and Monocytes

Distinct immunologic features of finnish Sjögren's syndrome patients with HLA alleles DRB10301, DQA10501, and DQB1*0201. Alterations in circulating T cell receptor γ/δ subsets

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Expression of CD45 isoforms by fresh and activated human γδ T lymphocytes and natural killer cells

Cited by 36 publications

References 0 publications

Lymphocyte activation and subset redistribution in the peripheral blood in acute malaria illness: distinct γδ+ T cell patterns in Plasmodium falciparum and P. vivax infections

Lymphocyte activation and subset redistribution in the peripheral blood in acute malaria illness: distinct γδ+ T cell patterns in Plasmodium falciparum and P. vivax infections

Identification and Characterization of a Novel Siglec, Siglec-7, Expressed by Human Natural Killer Cells and Monocytes

Distinct immunologic features of finnish Sjögren's syndrome patients with HLA alleles DRB1*0301, DQA1*0501, and DQB1*0201. Alterations in circulating T cell receptor γ/δ subsets

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Distinct immunologic features of finnish Sjögren's syndrome patients with HLA alleles DRB10301, DQA10501, and DQB1*0201. Alterations in circulating T cell receptor γ/δ subsets