Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana

Burssens, Sylvia; Himanen, Kristiina; Cotte, Brigitte van de; Beeckman, Tom; Montagu, Marc Van; Inzé, Dirk; Verbruggen, Nathalie

doi:10.1007/s004250000334

Cited by 175 publications

(129 citation statements)

References 43 publications

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“…To determine whether salt/osmotic stress inhibits root cell mitotic activity in stt3a seedlings, the expression of CycB1;1 was monitored ( Figure 4). CycB1;1 is expressed during the G2/M-phase transition and is a marker for cell division activity (Shaul et al, 1996;Burssens et al, 2000). Col stt3a-2 plants were crossed to a Col CycB1;1 -GUS (Colón-Carmóna et al, 1999) reporter line, and homozygous stt3a-2 F3 plants that exhibited salt/osmotic stress sensitivity were analyzed for ␤ -glucuronidase (GUS) expression.…”

Section: Isolation Of the Salt/osmotic Stress-sensitive Stt3a Mutantsmentioning

confidence: 99%

“…Together, these results are in agreement with the occurrence of UPR-induced cell cycle arrest and the involvement of STT3a in cell cycle progression during salt adaptation. Even in wild-type plants, severe osmotic stress can induce BiP expression (Cascardo et al, 2000) and temporal decline in cyclin expression (Burssens et al, 2000). Therefore, recovery from the UPR and the resumption of cell cycle progression appears to be an integral part of plant salt/osmotic stress adaptation, and the stt3a mutation disturbs these essential adaptation mechanisms.…”

Section: The Upr May Regulate Cell Cycle Progression During Osmotic Smentioning

confidence: 99%

“…Transport proteins that are targeted through the secretory system are essential for ion homeostasis (Shi et al, 2003). Although osmotic stress is implicated in the cell cycle arrest of plants (Burssens et al, 2000) as it is in yeast (Alexander et al, 2001), the mechanism(s) that coordinates osmosensing and plant cell cycle regulation has not been identified; consequently, its role in plant adaptation is not understood.…”

Section: Introductionmentioning

confidence: 99%

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The STT3a Subunit Isoform of the Arabidopsis Oligosaccharyltransferase Controls Adaptive Responses to Salt/Osmotic Stress

Koiwa¹

2003

THE PLANT CELL ONLINE

191

287

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Arabidopsis stt3a-1 and stt3a-2 mutations cause NaCl/osmotic sensitivity that is characterized by reduced cell division in the root meristem. Sequence comparison of the STT3a gene identified a yeast ortholog, STT3 , which encodes an essential subunit of the oligosaccharyltransferase complex that is involved in protein N -glycosylation. NaCl induces the unfolded protein response in the endoplasmic reticulum (ER) and cell cycle arrest in root tip cells of stt3a seedlings, as determined by expression profiling of ER stress-responsive chaperone ( BiP -GUS ) and cell division ( CycB1;1 -GUS ) genes, respectively. Together, these results indicate that plant salt stress adaptation involves ER stress signal regulation of cell cycle progression. Interestingly, a mutation ( stt3b-1 ) in another Arabidopsis STT3 isogene ( STT3b ) does not cause NaCl sensitivity. However, the stt3a-1 stt3b-1 double mutation is gametophytic lethal. Apparently, STT3a and STT3b have overlapping and essential functions in plant growth and developmental processes, but the pivotal and specific protein glycosylation that is a necessary for recovery from the unfolded protein response and for cell cycle progression during salt/osmotic stress recovery is associated uniquely with the function of the STT3a isoform.

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Section: Isolation Of the Salt/osmotic Stress-sensitive Stt3a Mutantsmentioning

confidence: 99%

Section: The Upr May Regulate Cell Cycle Progression During Osmotic Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The STT3a Subunit Isoform of the Arabidopsis Oligosaccharyltransferase Controls Adaptive Responses to Salt/Osmotic Stress

Koiwa¹

2003

THE PLANT CELL ONLINE

191

287

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“…Plants have shortened petioles, roots, and hypocotyls and a drastic decrease in lateral root formation. At the molecular level, salt treatment strongly reduced the expression of the cyclinA2;1 and cyclinB1;1 (Burssens et al, 2000b). Cell cycle progression can be also disturbed by oxidative stress.…”

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confidence: 99%

Differential Effect of Jasmonic Acid and Abscisic Acid on Cell Cycle Progression in Tobacco BY-2 Cells

et al. 2002

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Environmental stress affects plant growth and development. Several plant hormones, such as salicylic acid, abscisic acid (ABA), jasmonic acid (JA), and ethylene play a crucial role in altering plant morphology in response to stress. Developmental regulation often has the cell cycle machinery among its targets. We analyzed the effect of JA and ABA on cell cycle progression in synchronized tobacco (Nicotiana tabacum) BY-2 cells. Both compounds were found to prevent DNA replication, keeping the cells in the G1 stage, when applied just before the G1/S transition. However, ABA did not have any effect on subsequent phases of the cell cycle when applied at a later stage, whereas JA effectively prevented mitosis on application during DNA synthesis. This demonstrates that JA treatment can freeze synchronized BY-2 cells in both the G1 and G2 stages of the cell cycle. Jasmonate administered after the S-phase was less effective in decreasing the mitotic index, suggesting that cell sensitivity toward JA is dependent on the cell cycle phase. In cultures detained in the G2-phase, we observed a reduced histone H1 kinase activity of kinases associated with the p13 sucl protein.

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“…In Arabidopsis, the reduction in root meristem size and root growth during salt stress is correlated with the downregulation of CDC2a (cyclin-dependent kinase), CycA2;1 and CycB1;1 (mitotic cyclins) (11). The activity of cyclin-dependent kinase is diminished also by post-translational inhibition during salt stress (10).…”

Section: Effect Of Salinity On Plant Developmentmentioning

confidence: 99%

Salt Stress Signaling and Mechanisms of Plant Salt Tolerance

Chinnusamy

Zhu

Genetic Engineering: Principles and Methods

230

151

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INTRODUCTIONSoil salinity of agricultural land has led to the breakdown of ancient civilizations.Even today it threatens agricultural productivity in 77 mha of agricultural land, of which 45 mha (20% of irrigated area) is irrigated and 32 mha (2.1% of dry land) is unirrigated (1). Salinization is further spreading in irrigated land because of improper management of irrigation and drainage. Rain, cyclones and wind also add NaCl into the coastal agricultural land. Soil salinity often leads to the development of other problems in soils such as soil sodicity and alkalinity. Soil sodicity is the result of the binding of Na + to the negatively charged clay particles, which leads to clay swelling and dispersal. Hydrolysis of the Na-clay complex results in soil alkalinity. Thus, soil salinity is a major factor limiting sustainable agriculture.The USDA salinity laboratory defines saline soil as having electrical conductivity of the saturated paste extract (EC e ) of 4 dS m -1 (1 dS m -1 is approximately equal to 10 mM NaCl) or more. High concentrations of soluble salts such as chlorides of sodium, calcium and magnesium contribute to the high electrical conductivity of saline soils.NaCl contributes to most of the soluble salts in saline soil.The development of salinity-tolerant crops is the need of the hour to sustain agricultural production. Conventional breeding programs aimed at improving crop tolerance to salinity have limited success because of the complexity of the trait (2). Slow progress in breeding for salt-tolerant crops can be attributed to the poor understanding of the molecular mechanisms of salt tolerance. Understanding the molecular basis of plant salt tolerance will also help improve drought and extreme-temperature-stress tolerance, since osmotic and oxidative stresses are common to these abiotic stresses. The salt-2 tolerant mechanisms of plants can be broadly described as ion homeostasis, osmotic homeostasis, stress damage control and repair, and growth regulation (3). This chapter reviews recent progress in understanding salt-stress signaling and breeding/genetic engineering for salt -tolerant crops. EFFECT OF SALINITY ON PLANT DEVELOPMENTSalinity affects almost all aspects of plant development, including germination, vegetative growth and reproductive development. Soil salinity imposes ion toxicity, osmotic stress, nutrient (N, Ca, K, P, Fe, Zn) deficiency and oxidative stress on plants.Salinity also indirectly limits plant productivity through its adverse effects on the growth of beneficial and symbiotic microbes. High salt concentrations in soil impose osmotic stress and thus limit water uptake from soil. Sodium accumulation in cell walls can rapidly lead to osmotic stress and cell death (1). Ion toxicity is the result of replacement of K + by Na + in biochemical reactions, and Na + and Cl --induced conformational changes in proteins. For several enzymes, K + acts as cofactor and cannot be substituted by Na + .High K + concentration is also required for binding tRNA to ribosomes and thus protein synt...

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Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana

Cited by 175 publications

References 43 publications

The STT3a Subunit Isoform of the Arabidopsis Oligosaccharyltransferase Controls Adaptive Responses to Salt/Osmotic Stress

The STT3a Subunit Isoform of the Arabidopsis Oligosaccharyltransferase Controls Adaptive Responses to Salt/Osmotic Stress

Differential Effect of Jasmonic Acid and Abscisic Acid on Cell Cycle Progression in Tobacco BY-2 Cells

Salt Stress Signaling and Mechanisms of Plant Salt Tolerance

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