Expression of CILP-2 and DDR2 and ultrastructural changes in the articular cartilage of patients with knee osteoarthritis undergoing total knee arthroplasty: a pilot morphological study

Torga, Taavi; Suutre, Siim; Kisand, Kalle; Aunapuu, Marina; Arend, Andres

doi:10.1007/s00795-022-00339-4

Cited by 6 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering that other tissues in the body could contribute to the content of excreted C2C, it is important to investigate the expression and localization of this neoepitope directly in the damaged cartilage. This type of investigation is of significance not only for C2C, but also for other biomarkers to prove their pathogenic significance in the context of the disease [27,42]. It is worth emphasizing that, even though detected and measured in body fluids, the investigation and quantification of neoepitopes in articular cartilage have been the subject of limited attention within the existing literature, resulting in a restricted pool of studies available for consideration.…”

Section: Discussionmentioning

confidence: 99%

Cartilage Collagen Neoepitope C2C Expression in the Articular Cartilage and Its Relation to Joint Tissue Damage in Patients with Knee Osteoarthritis

Torga,

Suutre,

Kisand

et al. 2024

Biomedicines

Self Cite

View full text Add to dashboard Cite

Pathological cleavage of type II collagen (Col2) and generation of Col2 neoepitopes can serve as useful molecular markers of the progression of osteoarthritis (OA). One of such potential biomarkers is type II collagen neoepitope C2C. The aim of this study was to correlate the degree of articular cartilage damage in OA patients with C2C expression in histological samples of tissues removed during total knee replacement. Cartilage samples were obtained from 27 patients ranging in age from 55 to 66 years. In each patient, medial and lateral tibia plateau samples were analyzed according to the OARSI histopathology grading system. The C2C expression was evaluated on histological slides by semi-quantitative analysis using ImageJ Fiji 2.14.0 software. Spearman’s rank correlation analysis revealed a positive weak correlation (rho = 0.289, p = 0.0356) between the histological grade of tissue damage and the percentage of C2C staining. In addition, a highly significant positive correlation (rho = 0.388, p = 0.0041) was discovered between the osteoarthritis score (combining the histological grade of damage with the OA macroscopic stage) and the percentage of C2C staining in the samples. The C2C expression was detected in all the regions of the articular cartilage (i.e., the superficial zone, mid zone, deep zone and tidemark area, and the zone of calcified cartilage). Our findings imply that local expression of C2C correlates with the articular cartilage damage in OA-affected knees. This confirms that C2C can be used as a prospective marker for assessing pathological changes in the OA course and OA clinical trials.

show abstract

Section: Discussionmentioning

confidence: 99%

Cartilage Collagen Neoepitope C2C Expression in the Articular Cartilage and Its Relation to Joint Tissue Damage in Patients with Knee Osteoarthritis

Torga,

Suutre,

Kisand

et al. 2024

Biomedicines

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Cartilage Collagen Neoepitope C2C Expression in the Articular Cartilage and Its Relation with Joint Tissue Damage in Patients with Knee Osteoarthritis

Torga,

Suutre,

Aunapuu

et al. 2024

Preprint

View full text Add to dashboard Cite

Pathological cleavage of collagen type II (Col2) and generation of Col2 neoepitopes can be good molecular markers of progression of osteoarthritis (OA). One of such potential biomarkers is collagen type II neoepitope C2C. The aim of this study was to correlate the degree of articular cartilage damage in OA patients with C2C expression in histological samples of tissues removed during total knee replacement. Cartilage samples were obtained from 27 patients aged from 55 to 66. In each patient medial and lateral tibia plateau samples were analyzed according to the OARSI histopathology grading system. C2C expression was evaluated on histological slides by semi-quantitative analysis using ImageJ Fiji software. Spearman´s rank correlation analysis showed significant positive correlation (rho=0.289, P=0.0356) between the histological grade of tissue damage and the percentage of C2C staining. In addition, highly significant positive correlation (rho=0.388, P=0.0041) was found between osteoarthritis score (combining the histological grade of damage with the OA macroscopic stage) and the percentage of C2C staining in the samples. The C2C expression was found in all the regions of the articular cartilage (i.e. the superficial zone, mid zone, deep zone and tidemark area and the zone of calcified cartilage). Our study suggests that local expression of C2C correlates with the cartilage damage in the knee affected by OA. This further assures that C2C can be a perspective marker for evaluation of pathological processes in OA course and OA clinical trials.

show abstract

Osteoarthritis year in review 2023: metabolite and protein biomarkers

Liu,

Haque,

Huang

et al. 2023

Osteoarthritis and Cartilage

View full text Add to dashboard Cite

Expression of CILP-2 and DDR2 and ultrastructural changes in the articular cartilage of patients with knee osteoarthritis undergoing total knee arthroplasty: a pilot morphological study

Cited by 6 publications

References 32 publications

Cartilage Collagen Neoepitope C2C Expression in the Articular Cartilage and Its Relation to Joint Tissue Damage in Patients with Knee Osteoarthritis

Cartilage Collagen Neoepitope C2C Expression in the Articular Cartilage and Its Relation to Joint Tissue Damage in Patients with Knee Osteoarthritis

Cartilage Collagen Neoepitope C2C Expression in the Articular Cartilage and Its Relation with Joint Tissue Damage in Patients with Knee Osteoarthritis

Osteoarthritis year in review 2023: metabolite and protein biomarkers

Contact Info

Product

Resources

About