Expression of Claudin7 Is Tightly Associated with Epithelial Structures in Synovial Sarcomas and Regulated by an Ets Family Transcription Factor, ELF3

Abstract

Synovial sarcoma, a soft tissue sarcoma that develops in adults, is pathologically subclassified into monophasic spindle synovial sarcoma and biphasic synovial sarcoma with epithelial components. The molecular mechanism building the epithelial components in biphasic synovial sarcoma is totally unknown.

Transcriptional Control of Tight Junction Proteins via a Protein Kinase C Signal Pathway in Human Telomerase Reverse Transcriptase-Transfected Human Pancreatic Duct Epithelial Cells

“…52 It is reported that the expression of claudin-7 at epithelial structures in synovial sarcoma is regulated by ELF3. 53 Consistently, we demonstrated that ELF3 mRNA was increased in FBS-or TPA-treated cells and a pan-PKC inhibitor prevented up-regulation of ELF3 mRNA by TPA treatment. When knockdown of ELF3 was caused by siRNAs in hTERT-HPDE cells, up-regulation of claudin-7 by TPA was inhibited.…”

Identification of AFAP1L1 as a prognostic marker for spindle cell sarcomas

“…Immunohistochemical experiments using paraffin-embedded specimens of soft tissue sarcomas were performed as described previously (Kohno et al, 2006). The anti-AFAP1L1 antibody was used at a concentration of 1 mg/ml.…”

“…Western blot analyses were performed as described previously (Kohno et al, 2006). Membranes were incubated overnight with the anti-AFAP1L1 (1:1000), anti-Flag M2 (1:4000; SigmaAldrich) or anti-b-actin (1:4000; Sigma-Aldrich) antibody.…”

“…Reverse transcription and real-time qPCR RNA extraction and reverse transcription were performed as described previously (Kohno et al, 2006). Real-time qPCR analyses were performed with the ABI PRISM 7700 Sequence Detection System (Applied Biosystems, Carlsbad, CA, USA).…”

Expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma: association with features of differentiation

“…Hepatocyte nuclear factor 4α has been shown to induce expression of claudin 7 and promotion of its subcellular location at the tight junctional belt in F9 embryonic carcinoma cells 17. Additionally, claudin 7 expression is upregulated by ets family transcription factor ELF3 in synovial sarcoma 18. In colon epithelial cells, transcription factor tcf-4 downregulates claudin 7 through sox-9.…”