Expression of CLC-K chloride channels in the rat cochlea

Qu, Chunyan; Liang, Fang; Hu, Wei; Shen, Zhijun; Spicer, Samuel S.; Schulte, Bradley A.

doi:10.1016/j.heares.2005.12.012

Cited by 23 publications

(19 citation statements)

References 47 publications

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“…Similarly, simultaneous mutations of CLCNKA and CLCNKB also lead to Bartter's syndrome type 4, whereas mutations of CLCNKB lead to Bartter's syndrome without deafness (171,226,237). This observation is consistent with the finding that CLCNKA and CLCNKB are coexpressed in cells of the inner ear but not the kidney and with the notion that the two channels can substitute for each other in the inner ear (206) but not in the kidney (see below).…”

Section: K ϩ Cycling In the Inner Earsupporting

confidence: 82%

“…2A). Fibrocyte types II, IV, and V express Na ϩ /K ϩ -ATPase, the Na ϩ -2Cl Ϫ -K ϩ cotransporter SLC12A2 (NKCC1), and the Cl Ϫ channels CLCNKA and CLCNKB (36,154,177,206,230) (Fig. 2D).…”

Section: K ϩ Cycling In the Inner Earmentioning

confidence: 99%

“…2D). This Cl Ϫ conductance is composed of the Cl Ϫ channels CLC-NKA/BSND and CLCNKB/BSND (6,53,154,167,206,218,264,265). Cl Ϫ channels CLCNKA/BSND and CLCNKB/ BSND consist of the pore-forming CLCNKA ␣-subunits and CLCNKB and the BSND ␤-subunit (53,228).…”

Section: K ϩ Cycling In the Inner Earmentioning

confidence: 99%

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Functional significance of channels and transporters expressed in the inner ear and kidney

Läng

Vallon²,

Knipper³

et al. 2007

American Journal of Physiology-Cell Physiology

203

180

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A number of ion channels and transporters are expressed in both the inner ear and kidney. In the inner ear, K(+) cycling and endolymphatic K(+), Na(+), Ca(2+), and pH homeostasis are critical for normal organ function. Ion channels and transporters involved in K(+) cycling include K(+) channels, Na(+)-2Cl(-)-K(+) cotransporter, Na(+)/K(+)-ATPase, Cl(-) channels, connexins, and K(+)/Cl(-) cotransporters. Furthermore, endolymphatic Na(+) and Ca(2+) homeostasis depends on Ca(2+)-ATPase, Ca(2+) channels, Na(+) channels, and a purinergic receptor channel. Endolymphatic pH homeostasis involves H(+)-ATPase and Cl(-)/HCO(3)(-) exchangers including pendrin. Defective connexins (GJB2 and GJB6), pendrin (SLC26A4), K(+) channels (KCNJ10, KCNQ1, KCNE1, and KCNMA1), Na(+)-2Cl(-)-K(+) cotransporter (SLC12A2), K(+)/Cl(-) cotransporters (KCC3 and KCC4), Cl(-) channels (BSND and CLCNKA + CLCNKB), and H(+)-ATPase (ATP6V1B1 and ATPV0A4) cause hearing loss. All these channels and transporters are also expressed in the kidney and support renal tubular transport or signaling. The hearing loss may thus be paralleled by various renal phenotypes including a subtle decrease of proximal Na(+)-coupled transport (KCNE1/KCNQ1), impaired K(+) secretion (KCNMA1), limited HCO(3)(-) elimination (SLC26A4), NaCl wasting (BSND and CLCNKB), renal tubular acidosis (ATP6V1B1, ATPV0A4, and KCC4), or impaired urinary concentration (CLCNKA). Thus, defects of channels and transporters expressed in the kidney and inner ear result in simultaneous dysfunctions of these seemingly unrelated organs.

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Section: K ϩ Cycling In the Inner Earsupporting

confidence: 82%

Section: K ϩ Cycling In the Inner Earmentioning

confidence: 99%

See 1 more Smart Citation

Functional significance of channels and transporters expressed in the inner ear and kidney

Läng

Vallon²,

Knipper³

et al. 2007

American Journal of Physiology-Cell Physiology

203

180

View full text Add to dashboard Cite

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“…First, Cx26 was found to be widely expressed in the inner ear, including the spiral ligament, the stria vascularis and between supporting cells of the organ of Corti by immunohistochemistry methods [32,33]. In the proposed lateral K + recycling model, K + effluxing from the basolateral region of hair cells is returned to the endolymph via a pathway consisting of two distinct cell networks separated by the interstitial gaps [34][35][36][37][38]. The first of these networks, the epithelial cell network is composed of nonsensory cells in the organ of Corti and includes Deiters cells, Henson cells, Claudius cells, outer sulcus cells and their root processes.…”

Section: Discussionmentioning

confidence: 99%

Microarray-based mutation detection of pediatric sporadic nonsyndromic hearing loss in China

Sun

Shi

et al. 2012

International Journal of Pediatric Otorhinolaryngology

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“…That is, K + , Cl − cotransporter or Na + , K + , 2Cl − cotransporter mediate Cl − influx by cells bordering the interstitial spaces in the lateral K + recycling, while CLC-K, a voltage-dependent Cl − channel, is thought to function in the efflux of resorbed Cl − in the cells (Qu et al 2006;Hibino et al 2010). In this study, we tested whether TMEM16A/anoctamin 1 (ANO1), which was recently cloned and shown to be a Ca 2+ -activated Cl − channel (CaCC) (Caputo et al 2008;Schroeder et al 2008;Yang et al 2008), is expressed in the mouse cochlea.…”

Section: Introductionmentioning

confidence: 99%

Expression and immunohistochemical localization of TMEM16A/anoctamin 1, a calcium-activated chloride channel in the mouse cochlea

et al. 2011

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Sound transduction in the cochlea depends on the unique high concentrations of K(+) in the endolymph. The production and maintenance of high K(+) concentrations are accompanied by Cl(-) cycling. In this study, we report on an investigation of the expression and localization of TMEM16A/anoctamin 1 (ANO1), a recently cloned Ca(2+)-activated Cl(-) channel, in the mouse cochlea by Western blot and immunhistochemistry. The ANO1 protein was identified in the cochlea by Western blotting. The immunoreactivity was found in stria vascularis as a line and in the organ of Corti as three plaques. The cellular localization of ANO1 was examined by means of double-labeling experiments with anti-claudin 11, a marker for basal cells of the stria vascularis. The results demonstrated that ANO1 colocalized with claudin 11, indicating its expression in basal cells. We also examined ANO1 localization in the organ of Corti by double- and triple-labeling techniques with anti-myosin VI, a marker for hair cells, and anti-synaptophysin, a marker for olivocochlear efferent nerve endings under hair cells. The results clearly showed that ANO1 is colocalized with synaptophysin, but not with myosin VI, indicating that ANO1 is localized at medial olivocochlear efferent nerve endings under outer hair cells. These results suggest that ANO1 may be specifically involved in synaptic transmission from medial olivocochlear efferent nerve endings to outer hair cells in the organ of Corti, as well as Cl(-) cycling in basal cells of the stria vascularis.

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Expression of CLC-K chloride channels in the rat cochlea

Cited by 23 publications

References 47 publications

Functional significance of channels and transporters expressed in the inner ear and kidney

Functional significance of channels and transporters expressed in the inner ear and kidney

Microarray-based mutation detection of pediatric sporadic nonsyndromic hearing loss in China

Expression and immunohistochemical localization of TMEM16A/anoctamin 1, a calcium-activated chloride channel in the mouse cochlea

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