Expression of colony-stimulating factors and inflammatory cytokines in the uterus of CD1 mice during Days 1 to 3 of pregnancy

Sanford, Thomas; De, Mamata; Wood, Gary W.

doi:10.1530/jrf.0.0940213

Cited by 94 publications

(44 citation statements)

References 18 publications

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“…A surge of proinflammatory cytokines was observed in the stromal tissue of the endometrium within 1 h of insemination in mice (Sanford et al 1992, Robertson et al 1998 as well as in pigs (Rozeboom et al 1998, O'Leary et al 2004. The mRNA expression of IL1B, IL6, and leukemia inhibitory factor were increased 2-3-fold in the endometrium after stimulation with seminal plasma (Gutsche et al 2003).…”

Section: Sperm Survivability and Cytokines In Hen Oviductmentioning

confidence: 97%

Changes in the expression of interleukin-1β and lipopolysaccharide-induced TNF factor in the oviduct of laying hens in response to artificial insemination

Das¹,

Isobe²,

Yoshimura³

2009

Reproduction

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The aim of this study was to determine the physiological significance of interleukin-1b (IL1B) and lipopolysaccharide-induced TNF factor (LITAF) in the fate of sperm in the oviduct of laying hens after artificial insemination (AI ). Laying hens were inseminated with fresh semen, PBS or seminal plasma and tissues from different oviductal segments were collected to observe the general histology, changes in the mRNA expression of IL1B and LITAF and the localization of positive cells expressing immunoreactive IL1B (irIL1B). Semi-quantitative RT-PCR was used to observe the changes in mRNA expression of these molecules in the infundibulum, uterus, utero-vaginal junction (UVJ), and vagina after insemination. Intact sperm in the lumen and between the primary or secondary folds of the vagina were found until 6 h after insemination but were degraded at 12 h. The mRNA expression of IL1B and LITAF was significantly increased in the vagina until 6 h after AI but remained unchanged in the other oviductal segments. In the tissue of the vagina and UVJ, irIL1B was localized in the mucosal stroma. The number of irIL1B-positive cells was increased in the vagina but almost unchanged in UVJ after insemination with semen. Significant changes were not observed in the mRNA expression and irIL1B-positive cells in the vagina after PBS or seminal plasma insemination. The increase of IL1B and LITAF in the vagina may lead to sperm degradation and elimination by cilia of surface epithelium, whereas their lower levels in UVJ may permit sperm to survive in sperm storage tubules.

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Section: Sperm Survivability and Cytokines In Hen Oviductmentioning

confidence: 97%

Changes in the expression of interleukin-1β and lipopolysaccharide-induced TNF factor in the oviduct of laying hens in response to artificial insemination

Das¹,

Isobe²,

Yoshimura³

2009

Reproduction

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“…The elevated expression of TNF-a in the placenta and amniotic fluid has also been associated with pregnancy disorders such as spontaneous resorption in mice (Gendron et al 1990;Tangri and Raghupathy 1993) and spontaneous recurrent abortion (Lea et al 1997(Lea et al , 1998 and preeclampsia (Vince et al 1995;Kilpatrick 1999) in humans. Uterine TNF-a mRNA and protein is first detected at day 1 of pregnancy and its presence at a basal level appears to be necessary for successful pregnancy in the mouse (Sanford et al 1992;De et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Elevated concentration of TNF-? induces trophoblast differentiation in mouse blastocyst outgrowths

Whiteside

Boucaut

Teh

et al. 2003

Cell and Tissue Research

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Elevated expression of tumour necrosis factor-alpha (TNF-alpha) is associated with adverse pregnancy outcome. This study has examined the expression of TNF-alpha and its receptors (TNF-Rs) by mouse blastocysts and blastocyst outgrowths from day 4 to 9.5 of pregnancy and investigated the effects of elevated TNF-alpha on the inner cell mass (ICM) and trophoblast cells of blastocyst outgrowths. RT-PCR demonstrated TNF-alpha mRNA expression from day 7.5 to 9.5, TNF-R1 from day 6.5 to 9.5 and TNF-R2 from day 5.5 to 7.5 of pregnancy, and in situ hybridisation revealed the trophoblast giant cells (TGCs) of the early placenta as the site of TNF-alpha expression. Day 4 blastocysts were cultured in a physiologically high concentration of TNF-alpha (100 ng/ml) for 72 h to the outgrowth stage and then compared to blastocysts cultured in media alone. TNF-alpha-treated blastocyst outgrowths exhibited a significant reduction in ICM cells (mean +/- SD 23.90+/-10.42 vs 9.37+/-7.45, t-test, P<0.0001) with no significant change in the numbers of trophoblast cells (19.97+/-8.14 vs 21.73+/-7.79, t-test, P=0.39). Within the trophoblast cell population, the TNF-alpha-treated outgrowths exhibited a significant increase in multinucleated cells (14.10+/-5.53 vs 6.37+/-5.80, t-test, P<0.0001) and a corresponding significant decrease in mononucleated cells (5.87+/-3.60 vs 15.37+/-5.87, t-test, P<0.0001). In summary, this study describes the expression of TNF-alpha and its receptors during the peri-implantation period in the mouse. It also reports that elevated TNF-alpha restricts ICM proliferation in the blastocyst and changes the ratio of mononucleated to multinucleated trophoblast cells. These findings suggest a mechanism by which increased expression of TNF-alpha during trophoblast differentiation may be detrimental to pregnancy.

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“…Contrary to expectation, blocking ADM action at 1.5 hpc did not increase leukocyte and macrophage infiltrations in the uterus, which are signs of inflammation in response to semen [Marinoni et al 2007] or semen proteins [Sanford et al 1992;Robertson et al 1996]. ADM is known to have an early inflammatory effect by increasing lipopolysaccharide (LPS)-induced macrophage migration inhibitory factor (MIF) in macrophages [Wong et al 2005].…”

Section: Discussionmentioning

confidence: 67%

Possible functions of adrenomedullin from the seminal fluid in the female reproductive tract of the rat

Liao

Kong²,

Tang³

et al. 2012

Systems Biology in Reproductive Medicine

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Adrenomedullin (ADM) is found in male accessory sex glands and is part of the seminal secretion. It plays an important role in protecting the sperm in the female reproductive tract. In this study, we investigated the roles of ADM in inflammation and oxidative stress in the endometrium and in leukocyte and macrophage infiltration in the endometrial stroma. The expression of the ADM gene in the ventral prostate, coagulating gland, and seminal vesicle was determined by real time PCR. The peptide levels in the tissue and secretion were measured using an EIA Kit. The highest ADM mRNA and peptide levels were found in the ventral prostate. Most of the ADM in the seminal vesicle was stored in the tissue while little was secreted. The expression of the IL-1β gene and the secretion of TNFα and IL-6 in uterine tissue decreased significantly after treatment with ADM for 4 hours. Using an immunostaining method, the levels of leukocyte and macrophage infiltration were found to be lower at 24 hours post coitus than 1.5 hours post coitus. The infusion of ADM receptor antagonist reduced the infiltration of leukocyte and macrophages in the endometrial stroma at 24 hours post coitus. As to the anti-oxidative effect of ADM in the female tract, the reactive oxygen species (ROS) level in isolated endometrial epithelial cells was significantly decreased after treatment with ADM or seminal fluid. Our findings demonstrated that ADM in the seminal secretion may modify the inflammatory responses, play an anti-oxidative role, and increase leukocyte and macrophage infiltration in the uterus.

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Expression of colony-stimulating factors and inflammatory cytokines in the uterus of CD1 mice during Days 1 to 3 of pregnancy

Abstract: Further, the data demonstrate the expression of genes for CSF-1, GM-CSF, IL-1\g=a\,IL-1\g=b\, IL-6 and TNF-\g=a\ is induced in the uterus during mating.

Cited by 94 publications

References 18 publications

Changes in the expression of interleukin-1β and lipopolysaccharide-induced TNF factor in the oviduct of laying hens in response to artificial insemination

Changes in the expression of interleukin-1β and lipopolysaccharide-induced TNF factor in the oviduct of laying hens in response to artificial insemination

Elevated concentration of TNF-? induces trophoblast differentiation in mouse blastocyst outgrowths

Possible functions of adrenomedullin from the seminal fluid in the female reproductive tract of the rat

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