Expression of cornulin in oral premalignant lesions

Santosh, Neetha; McNamara, Kristin K.; Beck, F. Michael; Kalmar, John R.

doi:10.1016/j.oooo.2019.02.003

Cited by 18 publications

(14 citation statements)

References 34 publications

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“…According to our findings, CRNN expression was high (93.8%, Table 3B) in the non-malignant oral tissues but was reduced or absent in the primary TSCC tissue specimens and varied based on addiction status but was reduced in cases where cause was not recorded or unknown. This is in agreement with the previous studies in which CRNN expression has been reported to differentiate between low-grade and high-grade oral epithelial dysplasia and could be represented as a potential biomarker for the assessment of progression of oral cancers [21]. Up-regulated CRNN levels prevent lesion formation and its tumour suppressive role has been reported [22].…”

Section: Discussionsupporting

confidence: 93%

Expression of cornulin in tongue squamous cell carcinoma

Saleem¹,

Aleem²,

Atiq³

et al. 2021

ecancer

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The aim of the study is to identify cornulin (CRNN) protein expression associated with advancement of tongue squamous cell carcinoma (TSCC). A comparison of addictive (containing potential carcinogens) versus non-addiction causative agents was expected to allow detection of differences in CRNN expression associated with TSCC. Bespoke tissue microarrays (TMAs) were prepared and immunohistochemistry (IHC) performed to determine the changes in CRNN expression in epithelial cells of node-negative (pN-), node-positive (pN +) TSCC and non-cancer patients' oral tissues. TMAs were validated by performing IHC on whole diagnostic tissues. Chi-square test or Fisher's-exact tests were used to establish significant expression differences. Analogous analyses were performed for biomarkers previously associated with TSCC, namely collagen I alpha 2 (COL1A2) and decorin (DCN) to compare the significance of CRNN. Keratinisation and its level (low, extensive) were studied in relation to CRNN so that the extent of squamous differentiation could better be assessed. IHC immunoreactive score (IRS) clustered the patients based on weak/moderate (Low (IRS ≤ +3)) or strong (High (IRS ≥ +4)) expression groups. A low expression was observed in a larger number of patients in control proteins COL1A2 (77.3%), DCN (87.5%) and target protein CRNN (52.3%), respectively. Low CRNN expression was observed in TSCC where nodes were involved (pN+: mean 1.4 ± 2.1) (p = 0.248). Keratinisation (%) was low (0% ≤ 50%) in 42.2% and extensive (1% ≥ 50.0%) in 57.8% patients. In conclusion, our study suggested that Low CRNN expression was associated with grade and lymph node metastasis in TSCC. CRNN expression is independent of addiction, however potentially carcinogenic addictive substances might be aiding in the disease progression.

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Section: Discussionsupporting

confidence: 93%

Expression of cornulin in tongue squamous cell carcinoma

Saleem¹,

Aleem²,

Atiq³

et al. 2021

ecancer

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“…Cornulin is expressed during epidermal differentiation and engaged in different biological processes, such as cell communication, cell cycle, cellular processes, immune system processes, and response to stimulus (32,33). On proteomic analysis of OC tissue, dysplastic epithelial tissue, and normal epithelial tissue, cornulin was the most down-regulated protein in epithelial dysplasia and OC tissue samples (32,34). Furthermore, salivary cornulin was lower in their OC group than in the HC group, and had a high AUC (of 0.91) for differentiating between patients with OC and the HCs, although the sample size was small (32).…”

Section: Discussionmentioning

confidence: 99%

Identification of Salivary Proteomic Biomarkers for Oral Cancer Screening

Ishikawa

Ishizawa

Tanaka

et al. 2021

In Vivo

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“…It may be involved in the immune response and differentiation of the epidermis. Previous studies have reported that CRNN is downregulated in OSCC and esophageal squamous cell carcinoma [ 39 , 40 ]. Additionally, low expression of CRNN was associated with poor prognosis in OSCC [ 41 ].…”

Section: Discussion Of the Top 10 Dysregulated Genes In Osccmentioning

confidence: 99%

The integration of differentially expressed genes based on multiple microarray datasets for prediction of the prognosis in oral squamous cell carcinoma

2021

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Oral squamous cell carcinoma (OSCC) is a common human malignancy. However, its pathogenesis and prognostic information are poorly elucidated. In the present study, we aimed to probe the most significant differentially expressed genes (DEGs) and their prognostic performance in OSCC. Multiple microarray datasets from the Gene Expression Omnibus (GEO) database were aggregated to identify DEGs between OSCC tissue and control tissue. Least absolute shrinkage and selection operator (LASSO) Cox model was constructed to determine the prognostic performance of the aggregated DEGs based on The Cancer Genome Atlas (TCGA) OSCC cohort. Ten datasets with 341 OSCC samples and 283 control samples were included. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment revealed that the integrated DEGs were enriched in the IL-17 signaling pathway, viral protein interactions with cytokines and cytokine receptors, and amoebiasis, among others. Our LASSO Cox model was able to discriminate two groups with different overall survival in the training cohort and test cohort (p < 0.001). The time-dependent receiver operating characteristic (ROC) curve revealed that the area under the curve (AUC) values at one year, three years, and five years were 0.831, 0.898, and 0.887, respectively. In the testing cohort, the time-dependent ROC curve showed that the AUC values at one year, three years, and five years were 0.696, 0.693, and 0.860, respectively. Our study showed that the integrated DEGs of OSCC might be applicable in the evaluation of prognosis in OSCC. However, further research should be performed to validate our findings.

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Expression of cornulin in oral premalignant lesions

Cited by 18 publications

References 34 publications

Expression of cornulin in tongue squamous cell carcinoma

Expression of cornulin in tongue squamous cell carcinoma

Identification of Salivary Proteomic Biomarkers for Oral Cancer Screening

The integration of differentially expressed genes based on multiple microarray datasets for prediction of the prognosis in oral squamous cell carcinoma

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