Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse ?2(XI) collagen promoter

Smad7 Inhibits Chondrocyte Differentiation at Multiple Steps during Endochondral Bone Formation and Down-regulates p38 MAPK Pathways

“…These results suggest that 11Enh-Cre induces recombination from the step of round proliferative chondrocytes and that 11Prom-Cre induces recombination from the step of flat proliferative chondrocytes. The recombination pattern of our 11Enh-Cre transgenic mice was consistent with that of previously reported Cre transgenic mice containing identical promoter/enhancer sequences (30 (Fig. 3A).…”

How to build an inducible cartilage-specific transgenic mouse

“…Tsumaki and colleagues [16] showed that, depending on the deleted region of the Col11a2 promoter, it was possible to activate expression in neural tissues or even other random tissues, thereby further discriminating between a cartilage-specific element (-501 to -530) and a neural tissue-specific element. In 2005 Fujimuki and colleagues [17] reported that their Col11a2 -cre mouse did not show expression in non-cartilage tissues, including heart, brain and osteoblasts except for the notochord. They stated that the expression level of Col2a1 is higher, but that the Col11a2 -cre mouse showed higher specificity, potentially due to the lower expression levels.…”

“…Unfortunately, careful choice of elements provides no guarantees. Fujimaki and colleagues [17] showed that different founder mice using the same construct of Col11a1 -cre displayed different expression patterns, which could be due to physical or functional shortening of the promoter region during transgene integration or position effects at the site of integration. Some founders even showed no expression at all despite integration of the transgene.…”

Generation of a mouse line harboring a bi‐transgene expressing luciferase and tamoxifen‐activatable creERT2 recombinase in cartilage

“…Several gene promoters have been used to target chondrocytes. Prx1-Cre (Logan et al, 2002), Dermo1-Cre (Li et al, 1995), Gdf5-Cre (Rountree et al, 2004), and Sox9-Cre (Akiyama et al, 2005) target progenitor cells, whereas Col2a1-Cre (Ovchinnikov et al, 2000), Col10a1-Cre (Hilton et al, 2007), and Col11a2-Cre (Fujimaki et al, 2005) target differentiated chondrocytes. A drawback of some of these conditional systems has been that Cre expression occurs in nonchondrocytic cells during development (Fosang et al, 2013;Haigh et al, 2000;Kolpakova-Hart et al, 2008).…”

“…Prx1-Cre (Logan et al, 2002), Dermo1-Cre (Li et al, 1995), Gdf5-Cre (Rountree et al, 2004), and Sox9-Cre (Akiyama et al, 2005) target progenitor cells, whereas Col2a1-Cre (Ovchinnikov et al, 2000), Col10a1-Cre (Hilton et al, 2007), and Col11a2-Cre (Fujimaki et al, 2005) target differentiated chondrocytes. Prx1-Cre (Logan et al, 2002), Dermo1-Cre (Li et al, 1995), Gdf5-Cre (Rountree et al, 2004), and Sox9-Cre (Akiyama et al, 2005) target progenitor cells, whereas Col2a1-Cre (Ovchinnikov et al, 2000), Col10a1-Cre (Hilton et al, 2007), and Col11a2-Cre (Fujimaki et al, 2005) target differentiated chondrocytes.…”