Expression of cuticular protein genes, BmorCPG11 and BMWCP5 is differently regulated at the pre-pupal stage in wing discs of Bombyx mori

Ali, Md. Saheb; Wang, Huabing; Iwanaga, Masako; Kawasaki, Hideki

doi:10.1016/j.cbpb.2012.02.001

Cited by 14 publications

(32 citation statements)

References 40 publications

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“…Current studies have showed that BR-C, E74A and βFTZ-F1 regulate different cuticular protein genes [9,10,11,16,17,20]. Thus, these ecdysone-responsive transcription factors are suggested to regulate their target genes, and the series of their expression would bring about insect metamorphosis.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, cuticular protein genes, which have different developmental profiles, were induced by different types of ecdysone signal, and these things were described in a recent review [7].The determination of insect genomic sequences also brought about the comprehensive analysis of cuticular-protein gene expression and the analysis of its regulation by transcription factors [8][9][10][11][12][13][14][15][16][17]. Cuticular protein genes have different expression profiles [8][9][10][11][12][13][14][15][16][17] and regulatory systems by ecdysone-responsive transcription factors [9][10][11][12][13][14][15][16][17][18][19]. From this, insect cuticular protein genes are suggested to be a suitable material for clarifying the regulatory mechanism of ecdysone-responsive transcription factors.…”

Section: Introductionmentioning

confidence: 99%

“…From this, insect cuticular protein genes are suggested to be a suitable material for clarifying the regulatory mechanism of ecdysone-responsive transcription factors. Expression of CPR55, BMWCP2 and BMWCP5 was induced by an ecdysone pulse through a transcription factor, βFTZ-F1, which bound to their upstream binding sites and increased their promoter activity [10,16,17,20]. Recent experimental results proved that CPR95 was regulated by E74A and BmorCPG13 by BR-C Z2 through a reporter assay by using wing discs [9,11].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional Profiling Shows that BHR4 and E74A Are the Regulators of CPH33 and CPH34 Gene Expression

Ali¹,

Hossain²,

Mishra³

et al. 2018

JAMB

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Our endeavor is to identify with the basic system that regulates sequentially expressed cuticular protein genes around pupation. The present study was conducted to clarify the regulatory mechanism of a cuticular protein gene of Bombyx mori, BmorCPH33, whose expression in wing discs was atypical from others and peaked at wandering stage day 3 (W3) early to W3 late stage when ecdysteroid began to decrease before pupation. We compared the expression profiles of BmorCPH33 and BmorCPH34 to clarify their regulatory mechanism. Developmental profile of BmorCPH33 resembled BHR4, therefore we compared the expression profile of this transcription factor with that of BmorCPH33. BHR4 transcripts increased by the addition of 20E. BmorCPH33 expression was also induced by the addition of 20E, which was inhibited by cycloheximide. Both BHR4 and BmorCPH33 transcripts were induced after 6h 20E removal after exposure to 20E, but they were inhibited by the addition of cycloheximide. Thus, the results showed relatedness of BmorCPH33 and BHR4. Through this culture system it is suggested that BHR4 is a regulator of insect metamorphosis. The expression profiles of BmorCPH34 resembled those of E74A, therefore we compared the expression profiles of this transcription factor with BmorCPH34. BmorCPH34, E74A transcripts were up-regulated by the 20E pulse treatment. The results showed the relatedness of BmorCPH34 and E74A. The present findings showed different regulation of cuticular protein genes by ecdysone-responsive transcription factors at the pre-pupal stage of wing discs of B. mori.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptional Profiling Shows that BHR4 and E74A Are the Regulators of CPH33 and CPH34 Gene Expression

Ali¹,

Hossain²,

Mishra³

et al. 2018

JAMB

Self Cite

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“…Genome-wide analysis revealed the presence of 13 DnaJ homologues in silkworm (B. mori), and their phylogenetic analysis indicated that they evolved from a common ancestor but with variations, which may be due to evolution. The wing disc is a hemopoietic organ that shows dynamic morphogenesis during metamorphosis (Ali et al, 2012). The EST database revealed that Hsp40 has a higher expression in wing disc followed by brain and diapause destined egg, indicating its crucial role in the organ development during metamorphosis.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of DnaJ 5 homologs in silkworm Bombyx mori and its expression during egg diapause

et al. 2014

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A comparison of the cDNA sequences (1 056 bp) of Bombyx mori DnaJ 5 homolog with B. mori genome revealed that unlike in other Hsps, it has an intron of 234 bp. The DnaJ 5 homolog contains 351 amino acids, of which 70 contain the conserved DnaJ domain at the N-terminal end. This homolog of B. mori has all desirable functional domains similar to other insects, and the 13 different DnaJ homologs identified in B. mori genome were distributed on different chromosomes. The expressed sequence tag database analysis of Hsp40 gene expression revealed higher expression in wing disc followed by diapause-induced eggs. Microarray analysis revealed higher expression of DnaJ 5 homolog at 18th h after oviposition in diapause-induced eggs. Further validation of DnaJ 5 expression through qPCR in diapause-induced and nondiapause eggs at different time intervals revealed higher expression in diapause eggs at 18 and 24 h after oviposition, which coincided with the expression of Hsp70 as the Hsp 40 is its co-chaperone. This study thus provides an outline of the genome organization of Hsp40 gene, and its role in egg diapause induction in B. mori.

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“…Cuticles are also changed during metamorphosis 12 and cuticle proteins, like BmorCPG11 and BMWCP5 in the silkworm, are regulated by BR-C via 20E. [25][26][27][28] In Drosophila, the achaete-scute complex (AS-C) regulates bristle formation. 29 In studies where there was loss of complex function, Drosophila adults lost most of their bristles.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of lepidoptera scale cells from epidermal stem cells followed by ecdysone-regulated DNA duplication and scale secreting

et al. 2017

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Integuments are the first line to protect insects from physical damage and pathogenic infection. In lepidopteran insects, they undergo distinct morphology changes such as scale formation during metamorphosis. However, we know little about integument development and scale formation during this stage. Here, we use the silkworm, Bombyx mori, as a model and show that stem cells in the integument of each segment, but not intersegmental membrane, divide into two scale precursor cells during the spinning stage. In young pupae, the scale precursor cell divides again. One of the daughter cells becomes a mature scale-secreting cell that undergoes several rounds of DNA duplication and the other daughter cell undergoes apoptosis later on. This scale precursor cell division is crucial to the development and differentiation of scale-secreting cells because scale production can be blocked after treatment with the cell division inhibitor paclitaxel. Subsequently, the growth of scale-secreting cells is under the control of 20-hydroxyecdysone but not juvenile hormone since injection of 20-hydroxyecdysone inhibited scale formation. Further work demonstrated that 20-hydroxyecdysone injection inhibits DNA duplication in scale-secreting cells while the expression of scale-forming gene ASH1 was down-regulated by BR-C Z2. Therefore, this research demonstrates that the scale cells of the silkworm develops through stem cell division prior to pupation and then another wave of cell division differentiates these cells into scale secreting cells soon after entrance into the pupal stage. Additionally, DNA duplication and scale production in the scale-secreting cells were found to be under the regulation of 20-hydroxyecdysone.

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Expression of cuticular protein genes, BmorCPG11 and BMWCP5 is differently regulated at the pre-pupal stage in wing discs of Bombyx mori

Cited by 14 publications

References 40 publications

Transcriptional Profiling Shows that BHR4 and E74A Are the Regulators of CPH33 and CPH34 Gene Expression

Transcriptional Profiling Shows that BHR4 and E74A Are the Regulators of CPH33 and CPH34 Gene Expression

Molecular characterization of DnaJ 5 homologs in silkworm Bombyx mori and its expression during egg diapause

Differentiation of lepidoptera scale cells from epidermal stem cells followed by ecdysone-regulated DNA duplication and scale secreting

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