Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions

Köhler, I.; Ruiz‐Vera, Ursula M.; VanLoocke, Andy; Thomey, Michell L.; Clemente, Thomas E.; Long, Stephen P.; Ort, Donald R.; Bernacchi, Carl J.

doi:10.1093/jxb/erw435

Cited by 67 publications

(76 citation statements)

References 34 publications

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“…S2). The mean C i * here falls between the value of G* from tobacco (Nicotiana tabacum; Bernacchi et al, 2002) commonly used for soybean (Bernacchi et al, 2005;Rosenthal et al, 2014;Köhler et al, 2017) and a value calculated from soybean Rubisco kinetic properties (Gallé et al, 2013), and it further matches exactly the C i * estimated for soybean with similar methodology (Walker and Ort, 2015). Thus, the mean C i * value was used as a proxy for G* in all calculations.…”

Section: Parameters Used To Estimate G Mmentioning

confidence: 62%

Variable Mesophyll Conductance among Soybean Cultivars Sets a Tradeoff between Photosynthesis and Water-Use-Efficiency

Tomeo

Rosenthal

2017

Plant Physiol.

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Photosynthetic efficiency is a critical determinant of crop yield potential, although it remains below the theoretical optimum in modern crop varieties. Enhancing mesophyll conductance (i.e. the rate of carbon dioxide diffusion from substomatal cavities to the sites of carboxylation) may increase photosynthetic and water use efficiencies. To improve water use efficiency, mesophyll conductance should be increased without concomitantly increasing stomatal conductance. Here, we partition the variance in mesophyll conductance to within-and among-cultivar components across soybean (Glycine max) grown under both controlled and field conditions and examine the covariation of mesophyll conductance with photosynthetic rate, stomatal conductance, water use efficiency, and leaf mass per area. We demonstrate that mesophyll conductance varies more than 2-fold and that 38% of this variation is due to cultivar identity. As expected, mesophyll conductance is positively correlated with photosynthetic rates. However, a strong positive correlation between mesophyll and stomatal conductance among cultivars apparently impedes positive scaling between mesophyll conductance and water use efficiency in soybean. Contrary to expectations, photosynthetic rates and mesophyll conductance both increased with increasing leaf mass per area. The presence of genetic variation for mesophyll conductance suggests that there is potential to increase photosynthesis and mesophyll conductance by selecting for greater leaf mass per area. Increasing water use efficiency, though, is unlikely unless there is simultaneous stabilizing selection on stomatal conductance.

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Section: Parameters Used To Estimate G Mmentioning

confidence: 62%

Variable Mesophyll Conductance among Soybean Cultivars Sets a Tradeoff between Photosynthesis and Water-Use-Efficiency

Tomeo

Rosenthal

2017

Plant Physiol.

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“…In search of the greatest impact on yield, we used two constructs to achieve a range of H-protein overexpression. To achieve high expression levels, we used the CaMV 35S viral promoter which has been widely and successfully used in the past to drive high expression of the desired transgene and in the production of high yielding plants (Kohler et al, 2017;Lefebvre et al, 2005;Simkin et al, 2015). Additionally, the ST-LS1 promoter, previously used for the overexpression of GCS proteins in A. thaliana (Timm et al, 2012(Timm et al, , 2015, allowed us to achieve an intermediate level of overexpression in leaves.…”

Section: Discussionmentioning

confidence: 99%

Overexpressing the H‐protein of the glycine cleavage system increases biomass yield in glasshouse and field‐grown transgenic tobacco plants

López-Calcagno

Fisk

Brown

et al. 2018

Plant Biotechnology Journal

106

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SummaryPhotorespiration is essential for C3 plants, enabling oxygenic photosynthesis through the scavenging of 2-phosphoglycolate. Previous studies have demonstrated that overexpression of the L-and H-proteins of the photorespiratory glycine cleavage system results in an increase in photosynthesis and growth in Arabidopsis thaliana. Here, we present evidence that under controlled environment conditions an increase in biomass is evident in tobacco plants overexpressing the H-protein. Importantly, the work in this paper provides a clear demonstration of the potential of this manipulation in tobacco grown in field conditions, in two separate seasons. We also demonstrate the importance of targeted overexpression of the H-protein using the leaf-specific promoter ST-LS1. Although increases in the H-protein driven by this promoter have a positive impact on biomass, higher levels of overexpression of this protein driven by the constitutive CaMV 35S promoter result in a reduction in the growth of the plants. Furthermore in these constitutive overexpressor plants, carbon allocation between soluble carbohydrates and starch is altered, as is the protein lipoylation of the enzymes pyruvate dehydrogenase and alphaketoglutarate complexes. Our data provide a clear demonstration of the positive effects of overexpression of the H-protein to improve yield under field conditions.

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“…Although there was some variation in single seed weight from the samples collected at 138 different canopy positions in the present study ( Figure S1 and Köhler et al, 2016), these 139 differences were generally small. Across all treatments, comparison of single seed weight with 140 seed yield at the corresponding canopy position (i.e., bottom, middle or top position) produced a 141 positive correlation but with a very low correlation coefficient (r = 0.28) that was not statistically 142 significant.…”

Section: Plant Growth and Seed Yield 115mentioning

confidence: 66%

“…Canopy temperature measurements were 378 collected every 5 seconds to control the heater output, averaged every 10 min, and these values 379 were stored. For more information, see Köhler et al (2016) …”

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confidence: 99%

Increased temperatures may safeguard the nutritional quality of crops under future elevated CO₂ concentrations

Koehler

Bernacchi

Baxter

2018

Preprint

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2 Summary (244/250 words) 17Iron (Fe) and zinc (Zn) deficiencies are a global human health problem that may worsen 18 by growth of crops at elevated atmospheric CO2 concentration (eCO2). However, climate change 19 will also involve higher temperature, but it is unclear how the combined effect of eCO2 and 20 higher temperature will affect the nutritional quality of food crops. To begin to address this 21 question, we grew soybean (Glycine max) in a Temperature by Free-Air CO2 Enrichment (T-22 FACE) experiment in 2014 and 2015 under ambient (400 µmol mol -1 ) and elevated (600 µmol 23 mol -1 ) CO2 concentration and under ambient and elevated temperatures (+2.7 °C day and +3.4 °C 24 at night). In our study, eCO2 significantly decreased Fe concentration in soybean seeds in both 25seasons (-8.7% and -7.7%) and Zn concentration in one season (-8.9%) while higher temperature 26 (at ambient CO2 concentration) had the opposite effect. The combination of eCO2 with elevated 27 temperature generally restored seed Fe and Zn concentrations to levels obtained under ambient 28 CO2 and temperature conditions, suggesting that the potential threat to human nutrition by 29 increasing CO2 concentration may not be realized. In general, seed Fe concentration was 30 negatively correlated with yield suggesting inherent limitations to increasing seed Fe. In 31 addition, we confirm our previous report that the concentration of seed storage products and 32 several minerals varies with node position at which the seeds developed. Overall, these results 33 demonstrate the complexity of predicting climate change effects on food security when various 34 environmental parameters change in an interactive manner. 35

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Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions

Abstract: HighlightExpression of cyanobacterial FBP/SBPase in soybean prevents yield depression in a free air CO2 enrichment experiment under simulated future climate conditions with combination of elevated CO2 and elevated temperature.

Cited by 67 publications

References 34 publications

Variable Mesophyll Conductance among Soybean Cultivars Sets a Tradeoff between Photosynthesis and Water-Use-Efficiency

Variable Mesophyll Conductance among Soybean Cultivars Sets a Tradeoff between Photosynthesis and Water-Use-Efficiency

Overexpressing the H‐protein of the glycine cleavage system increases biomass yield in glasshouse and field‐grown transgenic tobacco plants

Increased temperatures may safeguard the nutritional quality of crops under future elevated CO₂ concentrations

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Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions

Abstract: HighlightExpression of cyanobacterial FBP/SBPase in soybean prevents yield depression in a free air CO2 enrichment experiment under simulated future climate conditions with combination of elevated CO2 and elevated temperature.

Cited by 67 publications

References 34 publications

Variable Mesophyll Conductance among Soybean Cultivars Sets a Tradeoff between Photosynthesis and Water-Use-Efficiency

Variable Mesophyll Conductance among Soybean Cultivars Sets a Tradeoff between Photosynthesis and Water-Use-Efficiency

Overexpressing the H‐protein of the glycine cleavage system increases biomass yield in glasshouse and field‐grown transgenic tobacco plants

Increased temperatures may safeguard the nutritional quality of crops under future elevated CO2 concentrations

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Increased temperatures may safeguard the nutritional quality of crops under future elevated CO₂ concentrations