Expression of cyclooxygenase 1 and 2 in the canine corpus luteum during diestrus

Kowalewski, Mariusz P.; Schuler, Gerhard; Taubert, Anja; Engel, Eva; Hoffmann, Bernd

doi:10.1016/j.theriogenology.2006.01.039

Cited by 101 publications

(151 citation statements)

References 22 publications

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“…Accordingly, total RNA from luteal tissue samples was isolated using TRIzol reagent following the manufacturer's protocol (Invitrogen) and as described in Kowalewski et al (2013). Semi-quantitative real-time (TaqMan) PCR was carried out in an automated fluorometer ABI PRISM 7500 Sequence Detection System (Applied Biosystems by Thermo Fisher) according to a previously described protocol (Kowalewski et al 2006).…”

Section: Expression Analysismentioning

confidence: 99%

“…The assays were set up to ensure approximately 100% efficiency of the PCRs. Relative gene expression was calculated using the comparative CT method (DDCT method) according to the manufacturer's protocols for the ABI PRISM 7500 Sequence Detection System (Applied Biosystems) and as described previously by Kowalewski et al (2006Kowalewski et al ( , 2011.…”

Section: Expression Analysismentioning

confidence: 99%

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LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

Lüttgenau¹,

Herzog²,

Strüve³

et al. 2016

Reproduction

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When given intravenously (iv), lipopolysaccharide (LPS) transiently suppresses the structure and function of the bovine corpus luteum (CL). This is associated with increased release of prostaglandin (PG) F 2a metabolite. The underlying regulatory mechanisms of this process remain, however, obscure. Therefore, the aims of this study were: i) to investigate the expression of the LPS receptor toll-like receptor 4 (TLR4) and 2 (TLR2) in the bovine CL during early, mid-and late luteal phases; and ii) to further dissect the mechanisms of LPS-mediated suppression of luteal function. As revealed by semi-quantitative qPCR and immunohistochemistry, both receptors were detectable throughout the luteal lifespan. Their mRNA levels increased from the early toward the mid-luteal phase; no further changes were observed thereafter. The TLR4 protein seemed more highly represented than TLR2. The cellular localization of TLRs was in blood vessels; weaker signals were observed in luteal cells. Additionally, cows were treated either with LPS (iv, 0.5 mg/kg BW) or with saline on Day 10 after ovulation. Samples were collected 1200 h after treatment and on Day 10 of the respective subsequent (untreated) cycle. The mRNA expression of several possible regulatory factors was investigated, revealing the suppression of PGF 2a receptor (PTGFR), STAR protein and 3b-hydroxysteroid dehydrogenase, compared with controls and subsequent cycles. The expression of TLR2 and TLR4, interleukin 1a (IL1A) and 1b (IL1B) and of PGF 2a and PGE 2 synthases (HSD20A and mPTGES respectively) was increased. The results demonstrate the presence of TLR2 and TLR4 in the bovine CL, and implicate their possible involvement in the deleterious effects of LPS on its function.

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Section: Expression Analysismentioning

confidence: 99%

Section: Expression Analysismentioning

confidence: 99%

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

Lüttgenau¹,

Herzog²,

Strüve³

et al. 2016

Reproduction

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“…For each sample, 100-200 ng DNase-treated total RNA were used in the RT, and cDNA was synthesized using RT reagents purchased from Applied Biosystems, with random hexamers used as primers according to our previously published protocol (Kowalewski et al 2006a(Kowalewski et al , 2011a. All reactions were carried out in an Eppendorf Mastercycler (Vaudaux-Eppendorf AG, Basel, CH, Switzerland).…”

Section: Rna Isolation and Reverse Transcription (Rt)mentioning

confidence: 99%

“…Thus, to provide required data on the mRNA level, molecular cloning and sequencing were carried out. Using an online available predicted sequence, canine-specific IGF1R primers were designed and ordered from Microsynth The GeneAmp Gold RNA PCR Kit from Applied Biosystems was used in a hot-start PCR according to our previously described protocol (Kowalewski et al 2006a(Kowalewski et al , 2011a. The annealing temperature was 58 8C.…”

Section: Homology Cloning Of Canine-specific Igf1rmentioning

confidence: 99%

“…In contrast to livestock, at least in nonpregnant bitches, there is no uterine luteolysin that could be required for normal ovarian cyclicity, because normal ovarian function is observed following hysterectomy (Olson et al 1984, Hoffmann et al 1992. Furthermore, a luteolytic role of intraluteally produced prostaglandins (PGs) can be ruled out (Kowalewski et al 2006a(Kowalewski et al , 2009). Thus, the absence of an acute luteolytic mechanism in the nonpregnant bitch (Concannon et al 1989, Hoffmann et al 1992) results in a physiological pseudopregnancy and a luteal life span similar to, or even longer than, that observed in pregnant bitches.…”

Section: Introductionmentioning

confidence: 99%

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Expression of genes involved in the embryo–maternal interaction in the early-pregnant canine uterus

et al. 2014

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Although there is no acute luteolytic mechanism in the absence of pregnancy in the bitch, a precise and well-timed embryo-maternal interaction seems to be required for the initiation and maintenance of gestation. As only limited information is available about these processes in dogs, in this study, the uterine expression of possible decidualization markers was investigated during the pre-implantation stage (days 10-12) of pregnancy and in the corresponding nonpregnant controls. In addition, the expression of selected genes associated with blastocyst development and/or implantation was investigated in embryos flushed from the uteri of bitches used for this study (unhatched and hatched blastocysts). There was an upregulated expression of prolactin receptor (PRLR) and IGF2 observed pre-implantation. The expression of PRL and of IGF1 was unaffected, and neither was the expression of progesterone-or estrogen receptor b (ESR2). In contrast, (ESR1) levels were elevated during early pregnancy. Prostaglandin (PG)-system revealed upregulated expression of PGE2-synthase and its receptors, PTGER2 and PTGER4, and of the PG-transporter. Elevated levels of AKR1C3 mRNA, but not the protein itself, were noted. Expression of prostaglandin-endoperoxide synthase 2 (PTGS2) remained unaffected. Most of the transcripts were predominantly localized to the uterine epithelial cells, myometrium and, to a lesser extent, to the uterine stroma. PGES (PTGES) mRNA was abundantly expressed in both groups of embryos and appeared higher in the hatched ones. The expression level of IGF2 mRNA appeared higher than that of IGF1 mRNA in hatched embryos. In unhatched embryos IGF1, IGF2, and PTGS2 mRNA levels were below the detection limit.

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The Dog: Nonconformist, Not Only in Maternal Recognition Signaling

Kowalewski

Gram

Kautz

et al. 2015

Regulation of Implantation and Establishment of Pregnancy in Mammals

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Although similar at the molecular and cellular levels, endocrine mechanisms governing reproductive function in the domestic dog (Canis familiaris) differ markedly at the regulatory level from those known in other domestic animal species. Some of the events, e.g., the lack of luteolysis in the absence of pregnancy, resulting in similar luteal function and, therefore, hormonal profiles in early pregnant and nonpregnant animals, are species-specific. Consequently, no early gestation marker has so far been identified for the dog. Following implantation, relaxin of fetal placental origin can be detected and used for pregnancy diagnosis. Characterized by the lack of an active luteolytic principle from intra- or extra-luteal sources, the canine reproductive cycle appears to represent a "basic" form of mammalian reproductive function with apparently reduced opportunities for facilitating fecundity and hastening reproduction. Nevertheless, in the dog some kind of mechanism for synchronization between blastocyst development and uterine preparation for pregnancy must have evolved in order to support gestation. Driven by this assumption, studies including our recent investigations have been initiated aimed at characterizing some of the embryo-mediated effects of the preimplantation embryo on the canine uterus. Moreover, the lack of a uterine luteolysin and consequently the absence of a need to develop an antiluteolytic strategy make the dog an interesting model for investigating early evolutionary mechanisms involved in the preparation for implantation and ensuring embryo survival. These mechanisms result in an inverse relationship between the duration of pregnancy and of the nonpregnant cycle in the dog, compared with all other domestic animal species.

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Expression of cyclooxygenase 1 and 2 in the canine corpus luteum during diestrus

Cited by 101 publications

References 22 publications

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

Expression of genes involved in the embryo–maternal interaction in the early-pregnant canine uterus

The Dog: Nonconformist, Not Only in Maternal Recognition Signaling

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