Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible involvement in ovulation

Fujimori, Chika; Ogiwara, Katsueki; Hagiwara, Akane; Rajapakse, Senaka; Kimura, Atsushi; Takahashi, Takayuki

doi:10.1016/j.mce.2010.09.015

Cited by 67 publications

(52 citation statements)

References 57 publications

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“…They include trout ovulatory protein-2 mRNA [64], [65]; amago salmon 20β-hydroxysteroid dehydrogenase activity [66]; medaka star and cyp17a1 mRNAs [6]; rainbow trout serine protease 23, adam22 , cscl14 , fgf2 , and ace2 mRNAs [67]; medaka prostaglandin E2 receptor mRNA [68]; and channel catfish cyp11a , cyp17a1 , cyp19 , and hsd3b mRNAs [44]. Among the genes and proteins described above, the channel catfish cyp11a , cyp17a1 , cyp19 , and hsd3b mRNAs are up-regulated by Lh [44].…”

Section: Discussionmentioning

confidence: 99%

“…Real-time PCR was performed using an Applied Biosystems 7300 Real-Time PCR System (Life technologies Inc., Rockville, MD). The PCR mixture preparation, PCR, and data analysis were carried out according to the previously reported protocol [68], [74]. To normalize the transcript levels of gonadotropin subunits (pituitary), gonadotropin receptors, metalloproteinases and their inhibitor (ovarian follicles), we tested the housekeeping genes cytoplasmic actin ( actb ), 18S rRNA ( rn18s1 ) and ribosomal protein L7 ( rpl7 ).…”

Section: Methodsmentioning

confidence: 99%

“…After hybridization, the membrane was washed twice in 2× saline sodium citrate (SSC) (0.15 M NaCl, 15 mM sodium citrate) containing 0.1% SDS at 65°C for 30 min and then washed twice in 0.1× SSC containing 0.1% SDS at 65°C for 30 min. As a control, medaka cytoplasmic actin ( actb ) mRNA was detected with a [α- 32 P] dCTP-labeled 312-bp actb cDNA fragment [68], [75]. The membrane was exposed to X-ray film (Kodak, Tokyo, Japan), which was then developed.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

et al. 2013

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The molecular properties and roles of luteinizing hormone (Lh) and its receptor (Lhcgrbb) have not been studied for the medaka (Oryzias latipes), which is an excellent animal model for ovulation studies. Here, we characterized the medaka Lh/Lhcgrbb system, with attention to its involvement in the ovulatory process of this teleost fish. In the medaka ovary, follicle-stimulating hormone receptor mRNA was expressed in small and medium-sized follicles, while lhcgrbb mRNA was expressed in the follicle layers of all growing follicles. Experiments using HEK 293T cells expressing medaka Lhcgrbb in vitro revealed that gonadotropin from pregnant mare’s serum and medaka recombinant Lh (rLh) bound to the fish Lhcgrbb. The fish gonadotropin subunits Gtha, Fshb, and Lhb were essentially expressed at fairly constant levels in the pituitary of the fish during a 24-h spawning cycle. Using medaka rLh, we developed a follicle culture system that allowed us to follow the whole process of oocyte maturation and ovulation in vitro. This follicle culture method enabled us to determine that the Lh surge for the preovulatory follicle occurred in vivo between 19 and 15 h before ovulation. The present study also showed that oocyte maturation and ovulation were delayed several hours in vitro compared with in vivo. Treatment of large follicles with medaka rLh in vitro significantly increased the expression of Mmp15, which was previously demonstrated to be crucial for ovulation in the fish. These findings demonstrate that Lh/Lhcgrbb is critically involved in the induction of oocyte maturation and ovulation.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

et al. 2013

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“…31,2012In humans, dexamethasone provides its therapeutic antiinflammatory benefit by acting on specific molecular targets, including the glucocorticoid receptor annexin, prostaglandin endoperoxide synthase 1, and prostaglandin endoperoxide synthase 2 [39][40][41]. Through gene expression analyses, annexin and prostaglandin endoperoxide synthase mRNA have been identified in the ovarian tissue of various fish species, providing evidence of potential involvement of these genes in gonad differentiation and ovulation, respectively [42][43][44]. When these genes were assessed in the fathead minnow tissue, there were no significant treatment differences, with the exception of the glucocorticoid receptor, which was increased in liver tissue from the 50 mg dexamethasone/L treatment group.…”

Section: Discussionmentioning

confidence: 99%

Effects of a glucocorticoid receptor agonist, dexamethasone, on fathead minnow reproduction, growth, and development

LaLone

Villeneuve

Olmstead

et al. 2012

Enviro Toxic and Chemistry

102

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Synthetic glucocorticoids are pharmaceutical compounds prescribed in human and veterinary medicine as anti-inflammatory agents and have the potential to contaminate natural watersheds via inputs from wastewater treatment facilities and confined animal-feeding operations. Despite this, few studies have examined the effects of this class of chemicals on aquatic vertebrates. To generate data to assess potential risk to the aquatic environment, we used fathead minnow 21-d reproduction and 29-d embryo-larvae assays to determine reproductive toxicity and early-life-stage effects of dexamethasone. Exposure to 500 µg dexamethasone/L in the 21-d test caused reductions in fathead minnow fecundity and female plasma estradiol concentrations and increased the occurrence of abnormally hatched fry. Female fish exposed to 500 µg dexamethasone/L also displayed a significant increase in plasma vitellogenin protein levels, possibly because of decreased spawning. A decrease in vitellogenin messenger ribonucleic acid (mRNA) expression in liver tissue from females exposed to the high dexamethasone concentration lends support to this hypothesis. Histological results indicate that a 29-d embryo-larval exposure to 500 µg dexamethasone/L caused a significant increase in deformed gill opercula. Fry exposed to 500 µg dexamethasone/L for 29 d also exhibited a significant reduction in weight and length compared with control fry. Taken together, these results indicate that nonlethal concentrations of a model glucocorticoid receptor agonist can impair fish reproduction, growth, and development.

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“…Numerous studies have also proved that ovulation, corpus luteum development and regression are mediated by PGs (78–80). To date, eight GPCRs for PGs have been identified in mammals.…”

Section: Heterodimers Among Reproduction – Associated Gpcrsmentioning

confidence: 99%

GPCR Heterodimerization in the Reproductive System: Functional Regulation and Implication for Biodiversity

Satake¹,

Matsubara²,

Aoyama³

et al. 2013

Front. Endocrinol.

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A G protein-coupled receptor (GPCR) functions not only as a monomer or homodimer but also as a heterodimer with another GPCR. GPCR heterodimerization results in the modulation of the molecular functions of the GPCR protomer, including ligand binding affinity, signal transduction, and internalization. There has been a growing body of reports on heterodimerization of multiple GPCRs expressed in the reproductive system and the resultant functional modulation, suggesting that GPCR heterodimerization is closely associated with reproduction including the secretion of hormones and the growth and maturation of follicles and oocytes. Moreover, studies on heterodimerization among paralogs of gonadotropin-releasing hormone (GnRH) receptors of a protochordate, Ciona intestinalis, verified the species-specific regulation of the functions of GPCRs via multiple GnRH receptor pairs. These findings indicate that GPCR heterodimerization is also involved in creating biodiversity. In this review, we provide basic and current knowledge regarding GPCR heterodimers and their functional modulation, and explore the biological significance of GPCR heterodimerization.

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Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible involvement in ovulation

Cited by 67 publications

References 57 publications

Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

Effects of a glucocorticoid receptor agonist, dexamethasone, on fathead minnow reproduction, growth, and development

GPCR Heterodimerization in the Reproductive System: Functional Regulation and Implication for Biodiversity

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