Expression of cyclooxygenase-2 related to angiogenesis in uterine cervical cancers

Fujimoto, Jiro; Toyoki, Hiroshi; Sato, Eriko; Sakaguchi, Hideki; Jahan, Israt; Alam, Syed Mahfuzul; Tamaya, Teruhiko

doi:10.1007/s11373-006-9114-6

Cited by 14 publications

(15 citation statements)

References 38 publications

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“…A significant correlation between microvessel counts, VEGF levels and COX-2 levels has been found in a study performed on patients with uterine cervical cancer. This study showed also a correlation between high Cox-2 expression and poor prognosis [87].…”

Section: Inflammation Angiogenesis and Car-cinogenesissupporting

confidence: 67%

Oxidative DNA Damage and Oxidant/Anti-Oxidant Enzymatic Systems in Carcinogenesis and Cancer Progression

Cavallo¹,

Ursini²

2007

CEI

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Several xenobiotics cause oxidative DNA damage by reactive oxygen species (ROS) induction. The antioxidant defence system, including antioxidant enzymes, radical scavengers and chain breakers, limits cell injury induced by ROS.In particular the antioxidants such as reduced glutathione (GSH) and ascorbic acid (AA) and the antioxidant enzymes superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutatione reductase (GR) are implicated in antioxidant cellular response. Oxidative stress occurs when there is an imbalance between production of ROS and cellular antioxidant capacity or when there is a decrease in this capacity. Failure of antioxidant systems may lead to mutagenic oxidative DNA damage as well as deregulation of cell cycle control, resulting in carcinogenesis. In the last years oxidative DNA damage as consequence of anti-oxidant system reduced activity or inhibition has been widely studied by comet test, a rapid and sensitive technique that allows to evaluate DNA damage and its repair on single whole cell. In particular Comet assay modified with Fpg (formamidopyrimidine glycosylase) or endo III enzymes, that recognize and cut oxidized DNA bases, allows to evaluate oxidative DNA damage measuring oxidized purines and pyrimidines respectively. Comet assay also permits to study oxidative DNA repair evaluating the persistence of oxidative DNA damage in relation to repair time, performing the test on cells immediately after induction of DNA damage and after DNA recovery time. Recent studies indicate that oxidant-generating enzymes such as inducible nitric oxide synthase (iNOS) and the inducible cyclooxygenase-2 (COX-2) are associated with growth and progression of tumour malignancy acting as mediators of inflammation, angiogenesis inductors and cancer proliferation promoters. It allowed to develop new anticancer treatments based on COX-2 inhibitors also in combination with radiation or antineoplastic drugs that may reduce side effects of cancer therapy.

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Section: Inflammation Angiogenesis and Car-cinogenesissupporting

confidence: 67%

Oxidative DNA Damage and Oxidant/Anti-Oxidant Enzymatic Systems in Carcinogenesis and Cancer Progression

Cavallo¹,

Ursini²

2007

CEI

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“…It has been shown repeatedly that Cox-2 expression is responsible for the angiogenesis associated with numerous types of cancer, including colon, 37 breast, 38 gastric, 39 uterine/cervical, 40 Wilms' Tumor 9 and Hodgkin's lymphoma. 41 In the investigation presented here, the expression of Cox-2 was indirectly lowered by the selective death of cells that overexpressed Cox-2 in the bladder.…”

Section: Discussionmentioning

confidence: 99%

Expression-targeted gene therapy for the treatment of transitional cell carcinoma

2008

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Targeted gene delivery for induced apoptosis of transitional cell carcinomas was carried out in vivo in mice via utilization of the murine cyclooxygenase type 2 (Cox-2) promoter (Tis10). MB49 cells, which constitutively overexpress Cox-2 like numerous other carcinomas, selectively expressed delivered genes that utilized this transcriptional control element. The products of the delivered genes were artificially inducible forms of caspases 3 and 9, which remained inactive until a chemical inducer of dimerization was later injected intraperitoneally. The genes were delivered intravesically as plasmids complexed with poly(ethylenimine). Significant improvements, in the form of reduced bladder mass, reduced tumor volume, anti-angiogenesis and inhibition of tumor growth were seen versus untreated or unactivated controls. In some instances, tumors were seen to go into complete remission. There were no apparent bystander effects associated with the treatments. This targeted gene therapy regimen could have wide applicability to numerous cancers due to constitutive overexpression of Cox-2.

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“…VEGF associated with COX-2 might work in the advancement of angiogenesis 31 . The expression of COX-2 has been shown to be induced by proinflammatory cytokines, and suggestions have been made that overexpression of COX-2 suppresses apoptosis and is directly related to tumor growth.…”

Section: Issn: 0975-8232mentioning

confidence: 99%

Untitled

2012

IJPSR

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Expression of cyclooxygenase-2 related to angiogenesis in uterine cervical cancers

Cited by 14 publications

References 38 publications

Oxidative DNA Damage and Oxidant/Anti-Oxidant Enzymatic Systems in Carcinogenesis and Cancer Progression

Oxidative DNA Damage and Oxidant/Anti-Oxidant Enzymatic Systems in Carcinogenesis and Cancer Progression

Expression-targeted gene therapy for the treatment of transitional cell carcinoma

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