Expression of cytochrome P450s in the liver of rats administered with Socheongryong-tang, a traditional herbal formula

Abstract

Objective:The purpose of this study was to investigate the potential influences of Socheongryong-tang (SCRT) on the messenger ribonucleic acid (mRNA) and protein expression of cytochrome P450 (CYP450) in vivo.Materials and Methods:SCRT was orally administered to either male or female Sprague-Dawley rats once daily at doses of 0, 1000, 2000, or 5000 mg/kg/day for 13 weeks. The mRNA expression of CYP450s (CYP1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2, and 4A1) in liver tissues was measured by reverse transcription polymerase chain reaction. And then, the protein expression of CYP1A1 and CYP2B1/2 in liver tissues was analyzed by the Western blot.Results:We found no significant influence in the mRNA expression of hepatic CYP1A2, 2C11, 2E1, 3A1, 3A2, and 4A1 after repeated administration of SCRT for 13 weeks. By contrast, the mRNA and protein expression of hepatic CYP1A1 was increased by repeated SCRT treatment in male rats, but not in female rats. The mRNA and protein expression of hepatic CYP2B1/2 in both genders was increased by administration of SCRT.Conclusion:A caution is needed when SCRT is co-administered with substrates of CYP2B1/2 for clinical usage. In case of male, an attention is also required when SCRT and drugs metabolized by CYP1A1 are taken together. Our findings provide information regarding the safety and effectiveness of SCRT when combined with conventional drugs.SUMMARY Oral administration of Socheongryong-tang for 13 weeks did not affect the mRNA expression of hepatic CYP1A2, 2C11, 2E1, 3A1, 3A2, and 4A1In male rats, oral administration of Socheongryong-tang for 13 weeks induced the mRNA and protein expression of hepatic CYP1A1 and CYP2B1/2In female rats, oral administration of Socheongryong-tang for 13 weeks induced the mRNA and protein expression of hepatic CYP2B1/2. Abbreviations used: SCRT: Socheongryong-tang, CYP450: Cytochrome P450, HPLC: High performance liquid chromatography, RT-PCR: Reverse transcription polymerase chain reaction.

Traditional Chinese medicine as supportive care for the management of liver cancer: Past, present, and future

“…HCC is characterized by uncontrolled cell proliferation. 49 Formulas such as son-you-yin 12 and so-cheong-ryong-tang 50 as well as herbs including Danshen 51 and Huangyaozi 52 significantly inhibit cell proliferation and tumor growth in HCC. The compounds isolated from herbs Raddeanin A, 53 and Ardipusilloside-I 54 also showed anti-proliferative effects in HCC cells.…”

A decellularized matrix enriched collagen microscaffold for a 3D in vitro liver model

“…38 CYP induction by drugs is one of the major side-effects of drug administration. 39,40 The activity of CYP genes also is an indication of functionality of hepatocytes. The major gene in rats for CYP450 activity is CYP1A1.…”

Drug resistance in recurrent and metastatic Ewing sarcoma

“…Certain gene polymorphisms in the CYP450 superfamily may cause intrinsic drug resistance by affecting protein function. 9,30 In Phase II, the drug binds glutathione, glucuronic acid, or sulfate via glutathione S -transferase (GST), UDP-glucuronosyl transferase, and sulfase catalysis, respectively and drug toxicity is attenuated. 9 In Phase III, metabolites are generated by efflux pumps such as P-gp and MRP family members.…”