Expression of cytokeratin 17 in normal and diabetic submandibular salivary gland (histological and immunohistochemical study)

Elabasiry, Magdy; Hassan, Sherif Sayed; Altohamy, Safaa M

doi:10.4103/tdj.tdj_25_18

Cited by 3 publications

(2 citation statements)

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“…Also, cytokeratin intermediate filaments show great fidelity in expression and are highly antigenic. 38 Distribution of cytokeratin 17 of parotid gland correlates with intercalated, striated, and excretory duct cells, while acinar cells have weak or no cytokeratin expression in their cytoplasm. 39 Our study aimed to determine the distribution of cytokeratin within the parenchymal elements of the parotid gland of diabetic animals compared with the atrophic gland due to duct ligation to study whether the effect of diabetes mellitus leads to explicit atrophy of the gland.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Study of Cytokeratin Immunostaining of Parotid Gland Parenchyma in Normal, Diabetic, and Excretory Duct Ligation of Mongrel Dogs

Hassan

Alqahtani

2022

Eur J Dent

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Objectives The present study aimed to give a glimpse of the normal distribution of intermediate filaments within the parotid gland parenchyma of mongrel dogs and to reveal the pathological changes that may occur as a result of the effects of diabetes mellitus or atrophy of the gland caused by the ligation of the excretory duct to discover whether there is a similarity in these pathological behaviors. Materials and Methods Twelve healthy mongrel dogs were used in the experiment and were divided into three groups: group I (the control group), group II (dogs with alloxan-induced diabetes), and group III (dogs with the right-side duct-ligated parotid gland). The dogs were sacrificed 45 days after the parotid excretory duct were tied. The right parotid gland of all groups was dissected and prepared for histological and immunohistochemical expression of cytokeratin 17 assay. Results Histological findings confirmed that the parotid gland parenchyma of the diabetic group had glandular atrophy characterized by the loss of gland structure, degenerated acini, and dilatation of the duct system. Moreover, there is a predominance of the fibrous component with the presence of fat cells within the gland compartments. On the contrary, the excretory duct-ligated group undergoes severe glandular atrophy of the previous character with the presence of duct-like structure as well as extravasation and vasodilatation. Immunohistochemical expression of cytokeratin 17 in control parotid using an immunoperoxidase technique showed that cytokeratin expression varies from negative to mild in all ducts and some serous acinar cells. The gland parenchyma of the diabetic group showed mild to strong cytokeratin expression of duct cells more concentrated in the apical part with moderate to strong expression of diffuse type in some serous acini. The intensity of cytokeratin 17 in gland compartments of the excretory duct-ligated group revealed a variation in expression that ranged from negative to strong diffuse staining throughout the gland. Conclusion The severity and prevalence of cytokeratin 17 in our results are predictive of the pathological influence of both diabetes mellitus and duct ligation on the cytokeratin intracellular filaments of the salivary gland parenchyma in a different way that interferes with saliva production and/or secretion leading to xerostomia.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Study of Cytokeratin Immunostaining of Parotid Gland Parenchyma in Normal, Diabetic, and Excretory Duct Ligation of Mongrel Dogs

Hassan

Alqahtani

2022

Eur J Dent

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Antox targeting AGE/RAGE cascades to restore submandibular gland viability in rat model of type 1 diabetes

Ahmad,

Hassan,

Saadawy

et al. 2024

Sci Rep

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Diabetes mellitus (DM) is a chronic disorder of glucose metabolism that threatens several organs, including the submandibular (SMG) salivary glands. Antox (ANX) is a strong multivitamin with significant antioxidant benefits. The goal of this study was to demonstrate the beneficial roles of ANX supplementation in combination with insulin in alleviating diabetic SMG changes. For four weeks, 30 rats were divided into equal five groups (n = 6): (1) control group; (2) diabetic group (DM), with DM induced by streptozotocin (STZ) injection (50 mg/kg i.p.); (3) DM + ANX group: ANX was administrated (10 mg/kg/day/once daily/orally); (4) DM + insulin group: insulin was administrated 1U once/day/s.c.; and (5) DM + insulin + ANX group: co-administrated insulin. The addition of ANX to insulin in diabetic rats alleviated hyposalivation and histopathological alterations associated with diabetic rats. Remarkably, combined ANX and insulin exerted significant antioxidant effects, suppressing inflammatory and apoptotic pathways associated with increased salivary advanced glycation end-product (AGE) production and receptor for advanced glycation end-product expression (RAGE) activation in diabetic SMG tissues. Combined ANX and insulin administration in diabetic rats was more effective in alleviating SMG changes (functions and structures) than administration of insulin alone, exerting suppressive effects on AGE production and frustrating RAGE downstream pathways.

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Early Alterations of Cytoskeletal Proteins Induced by Radiation Therapy in the Parenchymal Cells of Rat Major Salivary Glands: A Comparative Immunohistochemical Analysis

Hassan,

Alqahtani

2024

Cureus

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Expression of cytokeratin 17 in normal and diabetic submandibular salivary gland (histological and immunohistochemical study)

Cited by 3 publications

References 1 publication

Comparative Study of Cytokeratin Immunostaining of Parotid Gland Parenchyma in Normal, Diabetic, and Excretory Duct Ligation of Mongrel Dogs

Comparative Study of Cytokeratin Immunostaining of Parotid Gland Parenchyma in Normal, Diabetic, and Excretory Duct Ligation of Mongrel Dogs

Antox targeting AGE/RAGE cascades to restore submandibular gland viability in rat model of type 1 diabetes

Early Alterations of Cytoskeletal Proteins Induced by Radiation Therapy in the Parenchymal Cells of Rat Major Salivary Glands: A Comparative Immunohistochemical Analysis

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