Expression of Cytokeration 7 , 20 , 14 in Urothelial Carcinoma and Squamous Cell Carcinoma of the Egyprian Urinary Bladder Cancer

Hammam, Olfat; Wishahi, Mohamed; Khalil, Heba

doi:10.12816/0007876

Cited by 14 publications

(7 citation statements)

References 13 publications

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“…However, there are only two case reports describing the CK7 and CK20 expression pattern in UCVGD (3,8). Usually, the CK7 expression is higher than CK20 expression in the classical urothelial carcinoma or in the urothelial component of the UCVGD (10,17). Nonetheless, the literature is scarce in urothelial carcinoma with glandular differentiation.…”

Section: Discussionmentioning

confidence: 99%

Urothelial tumors with villous morphology: Histomorphology and role of immunohistochemistry in diagnosis

Mitra

Chatterjee

Das

et al. 2018

APMIS

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Villous adenoma and urothelial carcinoma with villoglandular differentiation (UCVGD) are rare urothelial tumours showing villous morphology, the former being a preneoplastic entity and the latter being a malignant one. The detailed immunohistochemistry of these entities is previously not described in the literature. Moreover, a limited biopsy sample of UCVGD or a villous adenoma with or without adenocarcinoma may be difficult to distinguish on the basis of the histomorphology alone. An immunohistochemical panel comprising of GATA3, p63, β-catenin, CK7 and CK20 was performed on five cases of UCVGD and three cases of villous adenoma with the aim of studying the expression of the proteins thereby aiding in the diagnosis of these entities in a limited surgical pathology specimen. The mean age of UCVGD was 66.8 years and all the patients were male. All the cases of UCVGD were associated with high grade papillary urothelial carcinoma with lamina propria invasion. The immunohistochemical panel showed strong nuclear GATA3 expression in the urothelial component of UCVGD. Interestingly, the high grade and the low grade villoglandular components of UCVGD also expressed GATA3 (nuclear) with a progressive loss of expression from the high grade to the low grade component. The villous adenomas showed negativity or aberrant cytoplasmic positivity for GATA3. The β-catenin showed a gradual loss of membranous expression from villous adenoma to low grade and high grade villoglandular components of UCVGD with a patchy membranous expression in the urothelial component of the UCVGD. p63 showed strong nuclear positivity in the urothelial component and uniform negativity in the villous adenoma and villoglandular component of UCVGD irrespective of its grade, thereby distinguishing the villoglandular component from the urothelial component. The urothelial component of UCVGD showed strong membranous CK7 expression and was higher than the CK20 expression in the urothelial component. In contrast, CK20 expression was higher in villous adenoma as compared to CK7. There was no difference in the expression of CK7 and CK20 in the villoglandular components and low grade and high grade villoglandular areas. The above-mentioned immunohistochemical pattern may help to distinguish the UCVGD from the villous adenoma.

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Section: Discussionmentioning

confidence: 99%

Urothelial tumors with villous morphology: Histomorphology and role of immunohistochemistry in diagnosis

Mitra

Chatterjee

Das

et al. 2018

APMIS

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“…Contrary to increased hepatic K8 and K18 levels in human liver disease [ 23 ], colonic K8, K18 and K19 levels have recently been reported to decrease in human colon during inflammatory stress, as observed in ulcerative colitis [ 35 ]. Furthermore, K7, K8 and K20 are increased in human colitis-associated dysplasia and colorectal cancer compared to healthy controls [ 36 , 37 , 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

Keratins Are Altered in Intestinal Disease-Related Stress Responses

Helenius

Antman

Asghar

et al. 2016

Cells

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Keratin (K) intermediate filaments can be divided into type I/type II proteins, which form obligate heteropolymers. Epithelial cells express type I-type II keratin pairs, and K7, K8 (type II) and K18, K19 and K20 (type I) are the primary keratins found in the single-layered intestinal epithelium. Keratins are upregulated during stress in liver, pancreas, lung, kidney and skin, however, little is known about their dynamics in the intestinal stress response. Here, keratin mRNA, protein and phosphorylation levels were studied in response to murine colonic stresses modeling human conditions, and in colorectal cancer HT29 cells. Dextran sulphate sodium (DSS)-colitis was used as a model for intestinal inflammatory stress, which elicited a strong upregulation and widened crypt distribution of K7 and K20. K8 levels were slightly downregulated in acute DSS, while stress-responsive K8 serine-74 phosphorylation (K8 pS74) was increased. By eliminating colonic microflora using antibiotics, K8 pS74 in proliferating cells was significantly increased, together with an upregulation of K8 and K19. In the aging mouse colon, most colonic keratins were upregulated. In vitro, K8, K19 and K8 pS74 levels were increased in response to lipopolysaccharide (LPS)-induced inflammation in HT29 cells. In conclusion, intestinal keratins are differentially and dynamically upregulated and post-translationally modified during stress and recovery.

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“…Also Parker et al (2003) confirmed the use of CK20 as tumor marker for screening and diagnosing bladder cancer rather than determining patients' prognosis. On the contrary, Ghaini et al (2012) and Hammam et al (2014) found reverse relationship between CK20 and stage or grade of bladder cancer.…”

Section: Discussionmentioning

confidence: 92%

Fibroblast growth factor receptor 1 and cytokeratin 20 expressions and their relation to prognostic variables in bladder cancer

Abdul-Maksoud

Shalaby

Elsayed

et al. 2016

Gene

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Expression of Cytokeration 7 , 20 , 14 in Urothelial Carcinoma and Squamous Cell Carcinoma of the Egyprian Urinary Bladder Cancer

Cited by 14 publications

References 13 publications

Urothelial tumors with villous morphology: Histomorphology and role of immunohistochemistry in diagnosis

Urothelial tumors with villous morphology: Histomorphology and role of immunohistochemistry in diagnosis

Keratins Are Altered in Intestinal Disease-Related Stress Responses

Fibroblast growth factor receptor 1 and cytokeratin 20 expressions and their relation to prognostic variables in bladder cancer

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