Expression of cytokines and chemokines and microvasculature alterations of the tongue from patients with chronic Chagas’ disease

Pereira, Sanívia Aparecida de Lima; Severino, Viviane Oliveira; Kohl, Narayane L. M.; Rodrigues, Denise Bertulucci Rocha; Alves, Polyanna Miranda; Clemente-Napimoga, Juliana Trindade; Reis, Marlene Antônia dos; Teixeira, Vicente P. A.; Napimoga, Marcelo Henrique

doi:10.1007/s00436-009-1513-y

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…Thus, the fact that the tongue does not present significant functional alterations answers a series of questions that have arisen in the development of several studies carried out by this team (Pereira et al, 2006;Pereira et al, 2007;Pereira et al 2009). However, it is clear that the present study presents limitations, such as the relatively small number of patients, since many of the patients that attended the clinic did not present CD or did not agree to participate in the study.…”

Section: Discussionmentioning

confidence: 99%

“…The tongue plays an essential role in deglutition (Felton et al, 2007). Previous studies have shown that in chronic chagasic patients the tongue presents several microscopic morphological alterations, such as increased vascular diameter, increased vascular wall area, increased blood vessel density, increased thickening of the basal capillary membrane (Pereira et al, 2009), as well as a higher percentage of collagen and higher density of mast cells (Pereira et al, 2007). Parasitism of the skeletal muscle in the chronic phase also triggers repair mechanisms through collagen deposition resulting in alterations in muscle contractility (Novaes et al, 2016).…”

Section: Introdutionmentioning

confidence: 99%

“…Although microscopic changes in the chagasic tongue have been described (Pereira et al, 2006;Pereira et al, 2007;Pereira et al 2009) and IgG antibodies anti-T. cruzi IgG have already been detected in infected patients (Barros et al, 1999), there are no studies evaluating lingual function and total antibody levels in the saliva of chagasic patients. Thus, the purpose of the present study was to evaluate the tongue function, salivary flow rate and total immunoglobulin A (IgA), immunoglobulin M (IgM) and immunoglobulin G (IgG) levels in the saliva of chronic chagasic patients in comparison with noninfected individuals.…”

Section: Introdutionmentioning

confidence: 99%

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TONGUE FUNCTION, SALIVARY FLOW RATE AND IgA, IgM AND IgG TOTAL SALIVARY LEVELS IN CHRONIC CHAGASIC PATIENTS

Martins¹,

Beghini²,

Barbosa³

et al. 2019

Rev Patol Trop

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Although microscopic alterations have been detected in tongues and salivary glands of chagasic patients and the identification of biomarkers in saliva has proved advantageous, there are no studies evaluating tongue function and total salivary IgA, IgG and IgM levels in chronic chagasic patients. The aim of this study was to evaluate tongue function, salivary flow rate, and total salivary IgA, IgG and IgM levels comparing chronic and non-chronic chagasic individuals. 37 patients were selected: chronic cardiac chagasic patients (n=6), chronic chagasic patients with the associated form of the disease (cardiopathy and megaesophagus) (n=11), and nonchagasic individuals (n=20). The tongue function underwent a phonoaudiological evaluation.The salivary f low rate was measured by sialometry. The total salivary IgA, IgG and IgM levels were evaluated by sandwich ELISA assay. Chagasic patients with the associated form of the disease presented higher salivary flow rate and lower salivary protein levels. No significant differences were noted in the lingual function or in the total salivary immunoglobulin levels among the groups. Although patients with chagasic megaesophagus presented higher levels of salivary flow and lower salivary protein, the fact that there were no significant differencesin lingual function and total salivary immunoglobulin levels among the groups led to the conclusion that chronic chagas disease does not modify the lingual function or the total IgA, IgG and IgM salivary levels. The present study was the first to evaluate the function of the tongue and salivary total immunoglobulin levels in Chagas disease.

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Section: Discussionmentioning

confidence: 99%

Section: Introdutionmentioning

confidence: 99%

Section: Introdutionmentioning

confidence: 99%

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TONGUE FUNCTION, SALIVARY FLOW RATE AND IgA, IgM AND IgG TOTAL SALIVARY LEVELS IN CHRONIC CHAGASIC PATIENTS

Martins¹,

Beghini²,

Barbosa³

et al. 2019

Rev Patol Trop

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“…In addition to the heart muscles, other muscles can be infected in human CD, such as the gastrocnemius (Lugo de Yarbuh et al 2006) and lingual muscles (Barbosa and Andrade 1984). Recent studies by our group have shown that the tongues of individuals with CD exhibit more significant morphological changes when compared with patients without CD (de Lima Pereira et al 2006Pereira et al , 2007Pereira et al , 2009.…”

Section: Introductionmentioning

confidence: 94%

Comparison between the collagen intensity and mast cell density in the lingual muscles and myocardium of autopsied chronic chagasic and nonchagasic patients

et al. 2012

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In chronic Chagas' disease (CD), an increase in collagen intensity and mast cell density has been described individually in the myocardium and tongue muscles. The aim of this study was to compare the percentage of collagen, mast cell tryptase (MCT) density, and mast cell chymase (MCH) density in the lingual muscles and myocardium from autopsied chagasic (CP) and nonchagasic patients (NCP). The selected cases were divided into two groups: (1) CP (n = 10) and (2) NCP (n = 10). Fragments were removed from the tongue and heart. After histological processing, the slices were stained with picrosirius, and immunohistochemistry was performed for MCH and MCT. The CP group showed the highest MCH and MCT densities and the highest percentage of collagen in the lingual muscles and myocardium when compared with the NCP group (p < 0.05). A significant positive correlation was observed between the collagen intensity and MCH density in the myocardium of the CP group. Although there are no reports in the literature of MCT and MCH in CD, its higher densities as well as higher percentage of collagen were found in the lingual muscles and myocardium in the CP group, suggesting that tryptase and chymase are associated with the pathogenesis of CD in these organs. Furthermore, the positive and significant correlation between the percentage of collagen and MCH density in the myocardium of the CP group suggests that the chymase is associated with fibrosis in CD, as demonstrated in other diseases.

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“…Therefore, since the tongue is an organ of easy access and presents resistance to decomposition and carbonization because it is located in a humid closed cavity, it has become an important organ in postmortem evaluation [7,8].…”

Section: Introductionmentioning

confidence: 99%

Morphometric Analysis of Tongue in Individuals of European and African Ancestry

2017

J Forensic Investigation

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The maxillary dental arch is generally preserved due to its relatively protected location, as well as due to the strength of the teeth because of cadaveric spasm. However, when the victim is edentulous, or when there are not enough comparative data to effectively analyze the dental arches, human identification information based on forensic dentistry methods is very limited. Therefore, since the tongue is an organ of easy access and presents resistance to decomposition and carbonization because it is located in a humid closed cavity, it has become an important organ in post-mortem evaluation. The objective of study was to compare the human tongue dimensions and collagen percentage according to ancestry, gender and age groups. Forty-five tongues were removed during autopsy performed between 1 and 8 hours after death and immediately fixed in formaldehyde in order to avoid decomposition. The tongue length, width and thickness of autopsied individuals were analyzed. Collagen percentage was determined using AxioVision software on slides stained with Picrosirius. The subjects were classified according to gender, age, and ancestry. The tongues length of African ancestry individuals were significantly longer than European ancestry tongues (p = 0.02). The European descendants showed significantly higher collagen percentage than the African descendants (p = 0.01). There was no significant difference regarding collagen percentage between gender groups or age groups. There were also no significant differences in tongue thickness and width according to the ancestry, gender or age groups. This study is the first to compare the size and collagen percentage of the human tongue between different gender, ethnic and age groups.In conclusion the African ancestry individuals were significantly longer and the European ancestry tongues had a higher collagen percentage. Therefore we suggested that tongue length and collagen percentage analysis could be used as additional parameter for human identification, especially in cases of quartering or even in high-impact accidents where isolated parts of the human body can be found far from the site of the murder or accident. However, further research are needed to confirm these findings.

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Expression of cytokines and chemokines and microvasculature alterations of the tongue from patients with chronic Chagas’ disease

Cited by 6 publications

References 38 publications

TONGUE FUNCTION, SALIVARY FLOW RATE AND IgA, IgM AND IgG TOTAL SALIVARY LEVELS IN CHRONIC CHAGASIC PATIENTS

TONGUE FUNCTION, SALIVARY FLOW RATE AND IgA, IgM AND IgG TOTAL SALIVARY LEVELS IN CHRONIC CHAGASIC PATIENTS

Comparison between the collagen intensity and mast cell density in the lingual muscles and myocardium of autopsied chronic chagasic and nonchagasic patients

Morphometric Analysis of Tongue in Individuals of European and African Ancestry

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