Expression of DCC in differentiating ameloblasts from developing tooth germs in rats

Kim, H.J.; Jeon, Soo-Kyung; Kang, Ji-Hyoun; Kim, M.S.; Ko, Hyun-Mi; Jung, Ji‐Yeon; Koh, Joon; Kim, W.J.; Lee, E.J.; Lim, Hee-Suk; Kim, S.H.

doi:10.1016/j.archoralbio.2009.03.003

Cited by 5 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Briefly, the 2nd molar germ at post-natal days 3, 6, and 9 was shown to be in the bell stage, crown formation stage, and root formation stage. The third molar germ at post-natal day 9 was in the cap stage (Kim et al, 2009). From the isolated germs, we also found that BMP2, DSPP, and DMP1 expression increased gradually as development progressed from the cap stage to the root formation stage, consistent with our previous findings.…”

Section: Discussionsupporting

confidence: 92%

SHP is Involved in BMP2-induced Odontoblast Differentiation

Hwang

Yang

et al. 2012

J Dent Res

Self Cite

View full text Add to dashboard Cite

Small Heterodimer Partner (SHP) interacts with diverse transcription factors such as Runx2 and regulates many cellular events including differentiation, proliferation, and energy metabolism. SHP is reported to be a positive regulator of BMP2-induced bone formation. This study aimed to clarify the role of SHP in odontoblast differentiation and matrix mineralization. Rat tooth germs were isolated, and gene expression was determined by RT-PCR and real-time PCR. Localization of SHP protein expression was identified by immunofluorescent analysis. Primary human dental pulp cells (HDPCs) were cultured with BMP2 and/or Ad-siSHP. Matrix mineralization was evaluated by Alizarin red staining. Transient transfection experiment was performed with the SHP or Dlx5 expressional plasmids and the DSPP gene. In tooth germs from post-natal days 3 to 9, BMP-2 and SHP expression increased with DSPP and DMP1 mRNA expression. In an immunostaining study, SHP was expressed in odontoblasts and surrounding osteoblasts. When HDPCs were cultured with BMP2 in mineralization-inducing medium, SHP expression also increased with an increase in DSPP expression. Down-regulation of SHP by Ad-siSHP inhibited matrix mineralization. In transient transfection experiments, overexpression of SHP was shown to enhance DSPP promoter activity through interactions between SHP and Dlx5. These results suggest that SHP may mediate BMP2 signaling to promote mineralization of the dentin matrix.

show abstract

Section: Discussionsupporting

confidence: 92%

SHP is Involved in BMP2-induced Odontoblast Differentiation

Hwang

Yang

et al. 2012

J Dent Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…1A, B). Rat tooth germs undergo different developmental stages with postnatal (PN) days: bell stage (PN 3), crown formation stage (PN 6), and root formation stage (PN 9) of maxillary second molar germs (Kim et al 2009). As tooth germ development progressed, the expression of COUP-TFII mRNA also increased along with the expression of ALP, DMP-1, DSPP, and homeoprotein Msx2 (Fig.…”

Section: Coup-tfii Expression In Odontoblast Lineage Cells and Rat Tooth Germmentioning

confidence: 99%

COUP-TFII Stimulates Dentin Sialophosphoprotein Expression and Mineralization in Odontoblasts

Hur

Jeong

et al. 2015

J Dent Res

View full text Add to dashboard Cite

Chicken ovalbumin upstream promoter transcription factor 2 (COUP-TFII), an orphan nuclear receptor belonging to the steroid-thyroid hormone receptor superfamily, plays an important role in cell fate determination of various tissues. However, the specific role of COUP-TFII in tooth development has not yet been elucidated. In the present study, we aimed to explore the role of COUP-TFII in dentin sialophosphoprotein (DSPP) expression and matrix mineralization in odontoblast-lineage cells. In primary human dental pulp cells (HDPCs) and murine dental papilla-derived cells (MDPC-23) cultured in a mineralizing medium, the expression of COUP-TFII was induced along with the increased odontoblast-specific dentin matrix protein-1 (DMP-1) and DSPP expression. Endogenous expression of COUP-TFII in maxillary second molar germs of rats showed an increasing tendency as development of the tooth progressed. Also, COUP-TFII protein was detected in greater quantity in the odontoblastic layer of second molar germs than in that of third molar germs of rats. Overexpression of COUP-TFII using an adenoviral system upregulated the expression of odontoblast-specific genes with increased alkaline phosphatase activity and matrix mineralization in odontoblast-lineage cells. In contrast, knockdown of COUP-TFII using small interfering RNA decreased the expression of odontoblast-specific genes, which reduced matrix mineralization. Mechanistic studies revealed that COUP-TFII increased DSPP transcription by direct binding on the DSPP promoter. In addition, COUP-TFII physically interacted with the homeodomain transcription factor Msx2 and antagonistically regulated the Msx2 effect on DSPP promoter activity. Taken together, these results suggest that COUP-TFII has a stimulatory role in DSPP expression and matrix mineralization in odontoblast-lineage cells.

show abstract

“…Previously, we identified the developmental stage of the rat tooth germ based on histological analysis (Kim et al, 2009). The 2nd molar tooth germ was at the bell stage, crown formation stage, and root formation stage, at post-natal days 3, 6, and 9, respectively.…”

Section: Expression Of Atf6 During Tooth Developmentmentioning

confidence: 99%

Transcriptional Factor ATF6 is Involved in Odontoblastic Differentiation

et al. 2014

Self Cite

View full text Add to dashboard Cite

ATF6 is an endoplasmic reticulum (ER) membrane-bound transcription factor that regulates various cellular functions. The purpose of this study was to investigate the role of ATF6 in odontoblast differentiation. Rat tooth germs were isolated, changes in gene expression were evaluated over time, and localization of ATF6 was determined by immunohistochemistry. Human dental pulp cells (HDPCs) were cultured with 50 µg/mL ascorbic acid and 5 mmol/L β-glycerophosphate or 100 ng/mL bone morphogenetic protein 2 to induce differentiation. Translocation of ATF6 was observed by immunofluorescence and confocal microscopy. Overexpression of ATF6 was performed with an adenoviral vector. Matrix mineralization was evaluated by alizarin red staining. Immunoreactivity to anti-ATF6 was observed in the odontoblastic layer of the molar tooth germ, and expressions of ATF6, dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP1) increased gradually during tooth germ development. When HDPCs were cultured in differentiation media, ATF6, DSPP, and DMP1 expression increased with the expression of unfolded protein response (UPR) markers, BiP and CHOP. Immunofluorescence results showed that ATF6 protein moved from cytoplasm to nucleus when cells were exposed to differentiation media. Notably, overexpression of ATF6 increased DSPP and DMP1 expression, alkaline phosphatase (ALP) activity, and matrix mineralization in HDPC cultures. Inhibition of ATF6 decreased ALP activity and mineralization. These results suggest that ER membrane-bound transcriptional factor ATF6 may be involved in odontoblastic differentiation.

show abstract

Expression of DCC in differentiating ameloblasts from developing tooth germs in rats

Cited by 5 publications

References 31 publications

SHP is Involved in BMP2-induced Odontoblast Differentiation

SHP is Involved in BMP2-induced Odontoblast Differentiation

COUP-TFII Stimulates Dentin Sialophosphoprotein Expression and Mineralization in Odontoblasts

Transcriptional Factor ATF6 is Involved in Odontoblastic Differentiation

Contact Info

Product

Resources

About