Expression of Delta1 and Serrate1 (Jagged1) in the mouse inner ear

Morrison, Alastair; Hodgetts, Christine; Gossler, Achim; Angelis, Martin Hrabé de; Lewis, Julian

doi:10.1016/s0925-4773(99)00066-0

Cited by 197 publications

(208 citation statements)

References 11 publications

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“…It was used at dilutions of 1:100 to 1:1000. Jagged1 (C-20) was used previously in mouse inner ear (Morrison et al 1999;Chen et al 2002;Brooker et al 2006). The same labeling pattern was seen with both anti-Jagged1 antibodies in whole-mount preparations of utricular and saccular macula.…”

Section: Antibodiesmentioning

confidence: 77%

“…Jagged1 (referred to as Serrate 1 in chick and SerrateA or Jagged1b in zebrafish) is a member of the Notch signaling pathway, a pathway known to play a pivotal role in the differentiation of HCs and SCs (reviewed in Kelley 2006a, b). Jagged1 is expressed in prosensory regions of the ear before cell differentiation (Adam et al 1998;Morrison et al 1999;Kiernan et al 2006). Like Sox2, Jagged1 signaling is required for the development of inner ear sensory organs (Kiernan et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Sox2 and Jagged1 Expression in Normal and Drug-Damaged Adult Mouse Inner Ear

Oesterle

Campbell

Taylor³

et al. 2007

JARO

218

245

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Inner ear hair cells detect environmental signals associated with hearing, balance, and body orientation. In humans and other mammals, significant hair cell loss leads to irreversible hearing and balance deficits, whereas hair cell loss in nonmammalian vertebrates is repaired by the spontaneous generation of replacement hair cells. Research in mammalian hair cell regeneration is hampered by the lack of in vivo damage models for the adult mouse inner ear and the paucity of cell-type-specific markers for nonsensory cells within the sensory receptor epithelia. The present study delineates a protocol to drug damage the adult mouse auditory epithelium (organ of Corti) in situ and uses this protocol to investigate Sox2 and Jagged1 expression in damaged inner ear sensory epithelia. In other tissues, the transcription factor Sox2 and a ligand member of the Notch signaling pathway, Jagged1, are involved in regenerative processes. Both are involved in early inner ear development and are expressed in developing support cells, but little is known about their expressions in the adult. We describe a nonsurgical technique for inducing hair cell damage in adult mouse organ of Corti by a single high-dose injection of the aminoglycoside kanamycin followed by a single injection of the loop diuretic furosemide. This drug combination causes the rapid death of outer hair cells throughout the cochlea. Using immunocytochemical techniques, Sox2 is shown to be expressed specifically in support cells in normal adult mouse inner ear and is not affected by drug damage. Sox2 is absent from auditory hair cells, but is expressed in a subset of vestibular hair cells. Double-labeling experiments with Sox2 and calbindin suggest Sox2-positive hair cells are Type II. Jagged1 is also expressed in support cells in the adult ear and is not affected by drug damage. Sox2 and Jagged1 may be involved in the maintenance of support cells in adult mouse inner ear.

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Section: Antibodiesmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Sox2 and Jagged1 Expression in Normal and Drug-Damaged Adult Mouse Inner Ear

Oesterle

Campbell

Taylor³

et al. 2007

JARO

218

245

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“…These included CD44, Fgfr3 (the mouse ortholog of human FGFR3 also known as CD333), and Jag1 (the mouse ortholog of JAG1 also known as human CD339), which were 6.2-, 11.6-, and 2.7-fold enriched in the cochlea, respectively (Table 1). Fgfr3 is recognized to be expressed in the developing pillar and Deiters' cells (Peters et al 1993), while Jag1 expression is restricted to most of the supporting cells in the developing auditory and vestibular systems (Morrison et al 1999). However, while CD44 was detected by in situ hybridization in the E15 mouse inner ear, it was not studied in later stages of development or associated with specific cell types in the auditory or vestibular sensory epithelia (Yu and Toole 1997).…”

Section: Resultsmentioning

confidence: 99%

CD44 is a Marker for the Outer Pillar Cells in the Early Postnatal Mouse Inner Ear

Hertzano

Puligilla

Chan

et al. 2010

JARO

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Cluster of differentiation antigens (CD proteins) are classically used as immune cell markers. However, their expression within the inner ear is still largely undefined. In this study, we explored the possibility that specific CD proteins might be useful for defining inner ear cell populations. mRNA expression profiling of microdissected auditory and vestibular sensory epithelia revealed 107 CD genes as expressed in the early postnatal mouse inner ear. The expression of 68 CD genes was validated with real-time RT-PCR using RNA extracted from microdissected sensory epithelia of cochleae, utricles, saccules, and cristae of newborn mice. Specifically, CD44 was identified as preferentially expressed in the auditory sensory epithelium. Immunohistochemistry revealed that within the early postnatal organ of Corti, the expression of CD44 is restricted to outer pillar cells. In order to confirm and expand this finding, we characterized the expression of CD44 in two different strains of mice with loss-and gain-of-function mutations in Fgfr3 which encodes a receptor for FGF8 that is essential for pillar cell development. We found that the expression of CD44 is abolished from the immature pillar cells in homozygous Fgfr3 knockout mice. In contrast, both the outer pillar cells and the aberrant Deiters' cells in the Fgfr3 P244R/+ mice express CD44. The deafness phenotype segregating in DFNB51 families maps to a linkage interval that includes CD44. To study the potential role of CD44 in hearing, we characterized the auditory system of CD44 knockout mice and sequenced the entire open reading frame of CD44 of affected members of DFNB51 families. Our results suggest that CD44 does not underlie the deafness phenotype of the DFNB51 families. Finally, our study reveals multiple potential new cell type-specific markers in the mouse inner ear and identifies a new marker for outer pillar cells.

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“…The molecular mechanisms underlying the development of the sensory lineage and the patterning of the or-gan of Corti have been studied extensively (Adam et al, 1998;Haddon et al, 1998;Lewis et al, 1998;Lewis, 1998;Bermingham et al, 1999;Lanford et al, 1999Lanford et al, , 2000Morrison et al, 1999;Riley et al, 1999;Eddison et al, 2000;Zhang et al, 2000;Zine et al, 2000Zine et al, , 2001Tsai et al, 2001;Bryant et al, 2002;Chen et al, 2002;Fekete and Wu, 2002;Radde-Gallwitz et al, 2004;Woods et al, 2004;Daudet and Lewis, 2005;Kiernan et al, 2005a,b;Matei et al, 2005). In particular, two classes of basic helix-loop-helix (bHLH)-containing factors, namely the positively and negatively acting transcription factors, have been shown to play essential roles in the differentiation of sensory hair cells and the patterning of the sensory mosaic in the organ of Corti (Bermingham et al, 1999;Zine et al, 2000Zine et al, , 2001Chen et al, 2002;Woods et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Together, these data suggest that Hes1 and Hes5 play an anti-neurogenic role for hair cell differentiation. It is hypothesized that Hes1 and Hes5 act downstream of activated Notch pathway (Jarriault et al, 1995;Chen et al, 1997;Lewis, 1998;Ohtsuka et al, 1999;Zine et al, 2001) to prevent the cells surrounding the hair cells from differentiating into the same cell type (Adam et al, 1998;Haddon et al, 1998;Lewis et al, 1998;Lanford et al, 1999;Morrison et al, 1999;Riley et al, 1999;Eddison et al, 2000;Zine et al, 2000;Zhang et al, 2000;Kiernan et al, 2001;Tsai et al, 2001;Kiernan et al, 2005b) and to restrict the boundary of the sensory epithelium (Daudet and Lewis, 2005).…”

Section: Introductionmentioning

confidence: 99%

Basic helix–loop–helix gene Hes6 delineates the sensory hair cell lineage in the inner ear

et al. 2006

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The basic helix-loop-helix (bHLH) gene Hes6 is known to promote neural differentiation in vitro. Here, we report the expression and functional studies of Hes6 in the inner ear. The expression of Hes6 appears to be parallel to that of Math1 (also known as Atoh1), a bHLH gene necessary and sufficient for hair cell differentiation. Hes6 is expressed initially in the presumptive hair cell precursors in the cochlea. Subsequently, the expression of Hes6 is restricted to morphologically differentiated hair cells. Similarly, the expression of Hes6 in the vestibule is in the hair cell lineage. Hes6 is dispensable for hair cell differentiation, and its expression in inner ear hair cells is abolished in the Math1-null animals. Furthermore, the introduction of Hes6 into the cochlea in vitro is not sufficient to promote sensory or neuronal differentiation. Therefore, Hes6 is downstream of Math1 and its expression in the inner ear delineates the sensory lineage. However, the role of Hes6 in the inner ear remains elusive.

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Expression of Delta1 and Serrate1 (Jagged1) in the mouse inner ear

Cited by 197 publications

References 11 publications

Sox2 and Jagged1 Expression in Normal and Drug-Damaged Adult Mouse Inner Ear

Sox2 and Jagged1 Expression in Normal and Drug-Damaged Adult Mouse Inner Ear

CD44 is a Marker for the Outer Pillar Cells in the Early Postnatal Mouse Inner Ear

Basic helix–loop–helix gene Hes6 delineates the sensory hair cell lineage in the inner ear

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