Expression of dnaK and groESL operons during sporulation of Bacillus megaterium

Hlaváček, Otakar; Adamec, Jiřı́; Vomastek, Tomáš; Babková, Lenka; Sedlák, Miloš; Vohradský, Jiřı́; Váchová, Libuše; Chaloupka, J.

doi:10.1016/s0378-1097(98)00274-2

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…The findings in this work allow three major conclusions. First, as reported by two other groups (13,21,28), a heat shock at an early time in sporulation results in an increase in wet heat resistance of the resultant spores. This effect does not appear to involve the heat shock response, as there was no elevation in the level of heat shock proteins in the spores with elevated wet heat resistance, as also found in a recent study (21), and we also found that mutations in several heat shock genes did not abolish this phenomenon.…”

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confidence: 71%

Heat Shock Proteins Do Not Influence Wet Heat Resistance of Bacillus subtilis Spores

Melly

Setlow

2001

J Bacteriol

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Spores of Bacillus subtilis are significantly more resistant to wet heat than are their vegetative cell counterparts. Analysis of the effects of mutations in and the expression of fusions of a coding gene for a thermostable ␤-galactosidase to a number of heat shock genes has shown that heat shock proteins play no significant role in the wet heat resistance of B. subtilis spores.The gram-positive bacterium Bacillus subtilis undergoes the process of sporulation when nutrients become exhausted, and the resulting spores are more resistant than are the growing cells to a variety of environmental insults including heat, UV and gamma radiation, and a number of toxic chemicals (8,34). Wet heat resistance is probably the most dramatic resistance property of dormant spores, as spores are resistant to about 40°C-higher temperatures than are vegetative cells (8). Spore wet heat resistance is due to a number of factors including dehydration of the spore protoplast or core (8), mineralization of the spore core (8), saturation of spore DNA with ␣/␤-type small acid-soluble proteins (33, 34), and thermal adaptation, as spores of a single species formed at higher temperatures are more wet heat resistant than are spores formed at lower temperatures (8, 39).Although many factors contribute to spore wet heat resistance, the identity of the target for wet heat killing of spores is not known. However, two different types of studies have indicated that spore DNA is not the killing target and suggested that some spore protein might be the target (2, 7, 30). If spore killing by wet heat is indeed through protein damage, then it is possible that repair or removal of a damaged protein might be important in spore wet heat resistance. Proteins that can repair or remove denatured proteins in vivo are often members of the heat shock regulon, which is important in the survival of many different bacteria after a heat shock (9,17). Since sporulation at elevated temperatures results in spores with increased heat resistance and heat shock protein synthesis is increased at elevated temperatures (12, 38), then spores prepared at higher temperatures may also have increased levels of heat shock proteins which may in turn contribute to their increased heat resistance. In order to investigate whether proteins of the heat shock regulon play any role in wet heat resistance of B. subtilis spores, we have examined (i) the effect of mutations in known heat shock genes on spore wet heat resistance, (ii) the effect of mild heat shock at various times during sporulation on spore wet heat resistance, and (iii) the expression of heat shock genes during germination of spore populations which had been killed ϳ50% by wet heat treatment.Effects of mutations in heat shock genes on spore wet heat resistance. The heat shock genes of B. subtilis are grouped into at least three classes based on the precise mechanism for the regulation of their expression (11); we examined the effects of mutations in representatives from each of the three classes. Mutations in class I genes...

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confidence: 71%

Heat Shock Proteins Do Not Influence Wet Heat Resistance of Bacillus subtilis Spores

Melly

Setlow

2001

J Bacteriol

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“…Furthermore, our proteomics pathway analysis revealed that protein chaperones, such as GroEL/S system, and proteins encoded by the heat shock dnaK gene cluster, were increased in relative abundance during the interaction. Previous studies have shown that sporulation in Bacillus megaterium does not increase the levels of GroEL and dnaK chaperones ( Hlaváček et al., 1998 ), which indicates a stress response in P. amylolyticus potentially related to pH drift.…”

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confidence: 90%

Metabolic Profiling of Interspecies Interactions During Sessile Bacterial Cultivation Reveals Growth and Sporulation Induction in Paenibacillus amylolyticus in Response to Xanthomonas retroflexus

Herschend

Ernst

Koren

et al. 2022

Front. Cell. Infect. Microbiol.

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The toolbox available for microbiologists to study interspecies interactions is rapidly growing, and with continuously more advanced instruments, we are able to expand our knowledge on establishment and function of microbial communities. However, unravelling molecular interspecies interactions in complex biological systems remains a challenge, and interactions are therefore often studied in simplified communities. Here we perform an in-depth characterization of an observed interspecies interaction between two co-isolated bacteria, Xanthomonas retroflexus and Paenibacillus amylolyticus. Using microsensor measurements for mapping the chemical environment, we show how X. retroflexus promoted an alkalization of its local environment through degradation of amino acids and release of ammonia. When the two species were grown in proximity, the modified local environment induced a morphological change and growth of P. amylolyticus followed by sporulation. 2D spatial metabolomics enabled visualization and mapping of the degradation of oligopeptide structures by X. retroflexus and morphological changes of P. amylolyticus through e.g. the release of membrane-associated metabolites. Proteome analysis and microscopy were used to validate the shift from vegetative growth towards sporulation. In summary, we demonstrate how environmental profiling by combined application of microsensor, microscopy, metabolomics and proteomics approaches can reveal growth and sporulation promoting effects resulting from interspecies interactions.

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Expression of dnaK and groESL operons during sporulation of Bacillus megaterium

Cited by 2 publications

References 17 publications

Heat Shock Proteins Do Not Influence Wet Heat Resistance of Bacillus subtilis Spores

Heat Shock Proteins Do Not Influence Wet Heat Resistance of Bacillus subtilis Spores

Metabolic Profiling of Interspecies Interactions During Sessile Bacterial Cultivation Reveals Growth and Sporulation Induction in Paenibacillus amylolyticus in Response to Xanthomonas retroflexus

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