Expression of dnak, groES and cps genes in irradiated Klebsiella pneumoniae strains isolated from UTI Egyptian patients

Nada, Hanady G.; Hagag, Sanaa A.; El-Tablawy, S. Y.

doi:10.21608/ejrsa.2018.5656.1054

Cited by 3 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using the Clinical and Laboratory Standards Institute (CLSI) guidelines [ 9 ], an antibiogram assay was performed on the isolated K. pneumoniae colonies. The antibiotic discs contained ampicillin (30 µg), cotrimoxazole (25 µg), cefixime (5 µg), cefotaxime (30 µg), ceftriaxone (30 µg), ceftazidime (30 µg), gentamicin (10 µg), amikacin (30 µg), tetracycline (30 µg), doxycycline (30 µg), minocycline (30 µg), tigecycline (15 µg), ciprofloxacin (5 µg), levofloxacin (5 µg), ampicillin/sulbactam (100/10 µg), piperacillin/tazobactam (100/10 µg), imipenem (10 µg), meropenem (10 µg), ertapenem (10 µg), doripenem (10 µg), aztreonam (30 µg), colistin (10 µg), and fosfomycin (200 µg) (Mast Diagnostics, United Kingdom) [ 4 , 10 ].…”

Section: Methodsmentioning

confidence: 99%

“…Klebsiella pneumoniae ( K. pneumoniae ) is one of the most important causes of urinary, respiratory, sepsis, and wound infections in communities and hospitals [ 1 , 2 ]. Multidrug-resistant (MDR) and extensively drug-resistant (XDR) K. pneumoniae are a leading cause of healthcare-associated infections, which are linked to rising medical expenditures and increased morbidity and mortality [ 3 , 4 ]. The emergence and dissemination of MDR and XDR K. pneumoniae isolates pose a significant threat to infection control programs and, therefore, require immediate attention [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Multidrug-resistant (MDR) and extensively drug-resistant (XDR) K. pneumoniae are a leading cause of healthcare-associated infections, which are linked to rising medical expenditures and increased morbidity and mortality [ 3 , 4 ]. The emergence and dissemination of MDR and XDR K. pneumoniae isolates pose a significant threat to infection control programs and, therefore, require immediate attention [ 4 , 5 ]. Carbapenems are the last-line for treatment to treat serious infections with MDR K. pneumonia [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High frequency of NDM-1 and OXA-48 carbapenemase genes among Klebsiella pneumoniae isolates in central Iran

Abbasi

Ghaznavi‐Rad

2023

BMC Microbiol

View full text Add to dashboard Cite

Background The emergence and distribution of multidrug-resistant (MDR) and carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a global health threat. Therefore, this study aimed to investigate the frequency and antibiotic resistance patterns of MDR, extensively drug-resistant (XDR), and CRKP, as well as the antibiotic resistance genes of Klebsiella pneumoniae (K. pneumoniae) isolates from patients’ infectious samples from central Iran. Methods This study examined 546 clinical samples of patients to identify K. pneumoniae. The isolates were investigated for their antibiotic resistance profile, extended-spectrum β-lactamase (ESBL), AMPC β-lactamase, carbapenemase resistance, sulfonamide, tetracycline, plasmid-mediated quinolone resistance (PMQR) along with their resistance genes, integrase, and quaternary ammonium compounds (qac) by polymerase chain reaction (PCR). Results Out of 546 clinical samples, 121 (22.1%) cases of K. pneumoniae were identified using culture and PCR methods. The highest antibiotic resistance rates were found for ampicillin (119/121; 98.3%), cotrimoxazole (78/121; 64.4%), and cefixime, cefotaxime, ceftriaxone, and ceftazidime as a group (77/121; 63.6%). Tigecycline, colistin, and fosfomycin were the most effective antimicrobial agents with 98.4%, 96.7%, and 95.9% susceptibility, respectively. The amount of CRKP was 51 (42.1%). All CRKP isolates were MDR. The most abundant genes were blaTEM (77/77; 100%), blaCTX−M1 (76/77; 98.7%), blaSHV (76/77; 98.7%), blaCTX−M15 (73/77; 94.8%) for ESBL; blaCIT 28 (48.3%) and blaCMY−2 26 (44.8%) for AMPC β-lactamase; and blaOXA−48 46 (90.1%) and blaNDM 36 (70.5%) for carbapenemase. Among the PMQR determinants, qnrB (25/52; 48%), qnrS (19/52; 36.5%), and qnrA (11/52; 21.1%) were positive from the isolates. TetA and tetB were recognized in 25 (44.6%) and 17 (30.3%) isolates, respectively. Class 1 and 2 integrons were recognized in 97 (80.1%) and 53 (43.8%) isolates, respectively. Conclusions Due to the high prevalence of MDR and CRKP in central Iran, tracking and immediate intervention are necessary for control and inhibition of K. pneumoniae resistant isolates. Tigecycline, colistin, and fosfomycin are the best treatment options for treatment of patients with CRKP in this geographical area.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High frequency of NDM-1 and OXA-48 carbapenemase genes among Klebsiella pneumoniae isolates in central Iran

Abbasi

Ghaznavi‐Rad

2023

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…The 13 chosen antibiotics include the following; cefoxitin (FOX, 30 µg), cefepime (FEP, 30 µg), ceftazidime (CAZ, 30 µg), ceftriaxon (CRO, 30 µg), amoxicillin/clavulanic acid (AMC, 30/10 µg), and imipenem (IPM, 10 µg) belonging to β-lactam antibiotics, piperacillin (PRL, 10 µg), gentamicin (CN, 10 µg), amikacin (AK, 30 µg),trimethoprim-sulfamethoxazole (SXT, 1.25/23.75 µg), ciprofloxacin (CIP, 5 µg), erythromycin (E, 15 µg), and polymixin (Pb30 µg). The test was performed as illustrated by [74] and the results were interpreted as resistant (R), intermediate (I) or sensitive (S), by measuring the diameter of the inhibition zone (DIZ) in mm around each disc in accordance with the recommendation of Clinical and Laboratory Standard Institute (CLSI, 2018). The antibiogram pattern of each isolate against the thirteen tested antibiotics was obtained and the multiple antibiotic resistance (MAR) index was calculated as follows:…”

Section: Antibiotic Susceptibility For E Coli O157 Isolatesmentioning

confidence: 99%

Detection of multidrug-resistant Shiga toxin-producing Escherichia coli in some food products and cattle faeces in Al-Sharkia, Egypt: one health menace

Nada,

El-Tahan,

El-Didamony

et al. 2023

BMC Microbiol

View full text Add to dashboard Cite

Background Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen, that is transmitted from a variety of animals, especially cattle to humans via contaminated food, water, feaces or contact with infected environment or animals. The ability of STEC strains to cause gastrointestinal complications in human is due to the production of Shiga toxins (sxt). However, the transmission of multidrug-resistance STEC strains are linked with a severity of disease outcomes and horizontal spread of resistance genes in other pathogens. The result of this has emerged as a significant threat to public health, animal health, food safety, and the environment. Therefore, the purpose of this study is to investigate the antibiogram profile of enteric E. coli O157 isolated from food products and cattle faeces samples in Zagazig City, Al-Sharkia, Egypt, and to reveal the presence of Shiga toxin genes stx1 and stx2 as virulence factors in multidrug-resistant isolates. In addition to this, the partial 16S rRNA sequencing was used for the identification and genetic recoding of the obtained STEC isolates. Results There was a total of sixty-five samples collected from different geographical regions at Zagazig City, Al-Sharkia—Egypt, which were divided into: 15 chicken meat (C), 10 luncheon (L), 10 hamburgers (H), and 30 cattle faeces (CF). From the sixty-five samples, only 10 samples (one from H, and 9 from CF) were identified as suspicious E. coli O157 with colourless colonies on sorbitol MacConkey agar media with Cefixime- Telurite supplement at the last step of most probable number (MPN) technique. Eight isolates (all from CF) were identified as multidrug-resistant (MDR) as they showed resistance to three antibiotics with multiple antibiotic resistance (MAR) index ≥ 0.23, which were assessed by standard Kirby-Bauer disc diffusion method. These eight isolates demonstrated complete resistance (100%) against amoxicillin/clavulanic acid, and high frequencies of resistance (90%, 70%, 60%,60%, and 40%) against cefoxitin, polymixin, erythromycin, ceftazidime, and piperacillin, respectively. Those eight MDR E. coli O157 underwent serological assay to confirm their serotype. Only two isolates (CF8, and CF13), both from CF, were showed strong agglutination with antisera O157 and H7, as well as resistance against 8 out of 13 of the used antibiotics with the highest MAR index (0.62). The presence of virulence genes Shiga toxins (stx1 and stx2) was assessed by PCR technique. CF8 was confirmed for carrying stx2, while CF13 was carrying both genes stx1, and stx2. Both isolates were identified by partial molecular 16S rRNA sequencing and have an accession number (Acc. No.) of LC666912, and LC666913 on gene bank. Phylogenetic analysis showed that CF8, and CF13 were highly homologous (98%) to E. coli H7 strain, and (100%) to E. coli DH7, respectively. Conclusion The results of this study provides evidence for the occurrence of E. coli O157:H7 that carries Shiga toxins stx1 and/or stx2, with a high frequency of resistance to antibiotics commonly used in human and veterinary medicine, in Zagazig City, Al-Sharkia, Egypt. This has a high extent of public health risk posed by animal reservoirs and food products with respect to easy transmission causing outbreaks and transfer resistance genes to other pathogens in animal, human, and plants. Therefore, environmental, animal husbandry, and food product surveillance, as well as, clinical infection control, must be strengthened to avoid the extra spread of MDR pathogens, especially MDR STEC strains.

show abstract

Evaluation of chemical composition, antioxidant, antibiofilm and antibacterial potency of essential oil extracted from gamma irradiated clove (Eugenia caryophyllata) buds

et al. 2021

View full text Add to dashboard Cite

Expression of dnak, groES and cps genes in irradiated Klebsiella pneumoniae strains isolated from UTI Egyptian patients

Cited by 3 publications

References 27 publications

High frequency of NDM-1 and OXA-48 carbapenemase genes among Klebsiella pneumoniae isolates in central Iran

High frequency of NDM-1 and OXA-48 carbapenemase genes among Klebsiella pneumoniae isolates in central Iran

Detection of multidrug-resistant Shiga toxin-producing Escherichia coli in some food products and cattle faeces in Al-Sharkia, Egypt: one health menace

Evaluation of chemical composition, antioxidant, antibiofilm and antibacterial potency of essential oil extracted from gamma irradiated clove (Eugenia caryophyllata) buds

Contact Info

Product

Resources

About