Expression of E-Cadherin/Beta-Catenin in Epithelial Carcinomas of the Thyroid Gland

Ivanova, Koni; Ananiev, Julian; Aleksandrova, Elina; Ignatova, M.; Gulubova, Maya

doi:10.3889/oamjms.2017.043

Cited by 14 publications

(18 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results thus confirm the efficacy of resveratrol to reverse RA resistance in ATC cells and suggest the potential clinical utility of this combination in the management of ATCs that are sensitive neither to resveratrol nor to RA. This notion is further supported by the redifferentiation tendency of resveratrol-treated THJ-11T in terms of increased Tg and E-cadherin production and, especially, the appearance of membranous E-cadherin localization that, as shown in the current study, are extremely low and even absent in undifferentiated and, especially, anaplastic thyroid cancers [ 26 , 27 , 28 , 29 ]. As the next step, the potential of resveratrol to improve I 131 uptake of ATC cells should be investigated.…”

Section: Discussionsupporting

confidence: 81%

Resveratrol Suppresses the Growth and Enhances Retinoic Acid Sensitivity of Anaplastic Thyroid Cancer Cells

Tian

et al. 2018

IJMS

View full text Add to dashboard Cite

Anaplastic thyroid cancer (ATC) is a highly lethal undifferentiated malignancy without reliable therapies. Retinoic acid (RA) has been employed to promote redifferentiation of thyroid cancers by increasing their I131 uptake and radio-sensitivity, but its effect(s) on ATCs has not yet been ascertained. Likewise, resveratrol induces cancer redifferentiation but, also in this case, its effects on ATCs remain unknown. These issues have been addresses in the current study using three human ATC cell lines (THJ-11T, THJ-16T, and THJ-21T) through multiple experimental approaches. The results reveal that RA exerts a small inhibitory effect on these cell lines. In comparison with normally cultured cells, the total cell number in resveratrol-treated THJ-16T and THJ-21T cultures significantly decreased (p < 0.05), and this effect was accompanied by reduced Cyclin D1 immuno-labeling, increased apoptotic fractions, and distinct caspase-3 activation. Resveratrol failed to inhibit growth but enhanced RA sensitivity of THJ-11T cells, suppressed peroxisome proliferator-activated receptor-β/δ (PPAR-β/δ), and upregulated cellular retinoic acid-binding protein 2 (CRABP2) and retinoic acid receptor beta (RAR-β) expression. Increased thyroglobulin (Tg) and E-cadherin levels and appearance of membranous E-cadherin were evidenced in resveratrol-treated THJ-11T cells. Our results demonstrate for the first time: (1) the therapeutic value of resveratrol by itself or in combination with RA in the management of ATCs, (2) the capacity of resveratrol to overcome RA resistance in ATC cells by reprogramming CRABP2/RAR- and fatty acid-binding protein 5 (FABP5)/PPAR-β/δ-mediated RA signaling, and (3) the redifferentiating potential of resveratrol in ATC cells.

show abstract

Section: Discussionsupporting

confidence: 81%

Resveratrol Suppresses the Growth and Enhances Retinoic Acid Sensitivity of Anaplastic Thyroid Cancer Cells

Tian

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…TGF-β induces the upregulation mesenchymal markers such as vimentin and downregulation of the epithelial marker E-cadherin, which are considered critical prerequisites for metastasis in numerous human cancers [72]. Therefore, it is not surprising that the expression of E-cadherin and β-catenin in thyroid cancer is associated with better prognosis [73]. Among the set of analyzed genes, TGFB1 was regulated differently in µg and in the presence of DEX.…”

Section: Cell Detachment In Microgravity and Epithelial-mesenchymal Tmentioning

confidence: 99%

Dexamethasone Inhibits Spheroid Formation of Thyroid Cancer Cells Exposed to Simulated Microgravity

Melnik

Sahana

Corydon

et al. 2020

Cells

View full text Add to dashboard Cite

Detachment and the formation of spheroids under microgravity conditions can be observed with various types of intrinsically adherent human cells. In particular, for cancer cells this process mimics metastasis and may provide insights into cancer biology and progression that can be used to identify new drug/target combinations for future therapies. By using the synthetic glucocorticoid dexamethasone (DEX), we were able to suppress spheroid formation in a culture of follicular thyroid cancer (FTC)-133 cells that were exposed to altered gravity conditions on a random positioning machine. DEX inhibited the growth of three-dimensional cell aggregates in a dose-dependent manner. In the first approach, we analyzed the expression of several factors that are known to be involved in key processes of cancer progression such as autocrine signaling, proliferation, epithelial–mesenchymal transition, and anoikis. Wnt/β-catenin signaling and expression patterns of important genes in cancer cell growth and survival, which were further suggested to play a role in three-dimensional aggregation, such as NFKB2, VEGFA, CTGF, CAV1, BCL2(L1), or SNAI1, were clearly affected by DEX. Our data suggest the presence of a more complex regulation network of tumor spheroid formation involving additional signal pathways or individual key players that are also influenced by DEX.

show abstract

“…As an important effector in the positive regulation of the Wnt signalling pathway, E-cadherin participates in signal transduction. 29 Downregulation of the protein expression of E-cadherin and upregulation of the expression of N-cadherin are distinguishing features of EMT, and these changes can enable tumour cells to acquire migration and invasion abilities. 30 Therefore, in this study, we explored the potential mechanism by which L. fermentum ZS09 inhibits the migration of colon cancer cells by blocking the EMT pathway.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus fermentum ZS09 Mediates Epithelial–Mesenchymal Transition (EMT) by Regulating the Transcriptional Activity of the Wnt/β-Catenin Signalling Pathway to Inhibit Colon Cancer Activity

Liu¹,

Chen²,

Zhou³

et al. 2021

JIR

View full text Add to dashboard Cite

The epithelial-mesenchymal transition (EMT) pathway can mediate tumour migration, and the occurrence of EMT is closely related to the Wnt/β-catenin signalling pathway. The purpose of this paper was to study the effect of Lactobacillus fermentum ZS09 (L. fermentum ZS09) on the EMT pathway in mouse with azoxymethane/dextran sulfate sodium salt (AOM/DSS) induced colon cancer and the potential underlying mechanism. Materials and Methods: In this study, a mouse colon cancer model was established through intraperitoneal injection of 10 mg/kg azoxymethane (AOM) and three cycles of 2.5% dextran sulfate sodium salt (DSS) in the drinking water. H&E staining, enzyme-linked immunosorbent assay (ELISA), real-time fluorescent quantitative PCR (RT-qPCR) and Western blotting (WB) were used to study the antitumour mechanisms of L. fermentum ZS09 through the EMT pathway. Results:The results of this study showed that compared with the model group, the highdose L. fermentum ZS09 intervention group exhibited decreased serum levels of MMP-9, TNF-α, IL-6R, Ang-2 and VEGFR-2 and increased contents of DKK1 (P<0.05). The expression of Wnt/β-catenin signalling pathway-related genes (Dv1, cyclinD1, Vim, was significantly reduced, and the gene expression levels of APC, CDH1, and Axin were increased. The levels of related proteins (β-catenin, N-cadherin, and VEGF) were downregulated, and the levels of p-β-catenin and E-cadherin were upregulated. Conclusion:The results indicate that L. fermentum ZS09 could inhibit EMT and angiogenesis pathways by inhibiting the Wnt/β-catenin signalling pathway, which could inhibit tumour metastasis.

show abstract

Expression of E-Cadherin/Beta-Catenin in Epithelial Carcinomas of the Thyroid Gland

Cited by 14 publications

References 24 publications

Resveratrol Suppresses the Growth and Enhances Retinoic Acid Sensitivity of Anaplastic Thyroid Cancer Cells

Resveratrol Suppresses the Growth and Enhances Retinoic Acid Sensitivity of Anaplastic Thyroid Cancer Cells

Dexamethasone Inhibits Spheroid Formation of Thyroid Cancer Cells Exposed to Simulated Microgravity

Lactobacillus fermentum ZS09 Mediates Epithelial–Mesenchymal Transition (EMT) by Regulating the Transcriptional Activity of the Wnt/β-Catenin Signalling Pathway to Inhibit Colon Cancer Activity

Contact Info

Product

Resources

About